Rabbit Recombinant Monoclonal SDF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide - Human, Human samples. Cited in 22 publications.
Images
![Western blot - Anti-SDF1 antibody [EPR1216] (AB155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--western-blot-img40889.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SDF1 antibody [EPR1216] (AB155090)")
![Flow Cytometry (Intracellular) - Anti-SDF1 antibody [EPR1216] (AB155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--flow-cytometry-intracellular-img138384.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SDF1 antibody [EPR1216] (AB155090)")
![Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (AB155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--immunocytochemistry-immunofluorescence-img23943.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (AB155090)")
![Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (AB155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--immunocytochemistry-immunofluorescence-img70764.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (AB155090)")
![Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (AB155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--immunocytochemistry-immunofluorescence-img402159.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (AB155090)")
Publications
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- Evidence-based complementary and alternative medicine : eCAM 2022:32190012022Curcumol Undermines SDF-1/CXCR4/NF-B Signaling Pathway to Suppress the Progression of Chronic Atrophic Gastritis (CAG) and Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuehui Ma,Lingjing Kong,Wen Zhu,Yongli Wang,Zhengbo Zhang,Yaozhou TianPubMed 36159583
- Advanced healthcare materials 11:e21027952022Combinatorial Use of Therapeutic ELP-Based Micelle Particles in Tissue Engineering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeyza Bulutoglu,Aylin Acun,Sarah L Deng,Safak Mert,Elise Lupon,Alexandre G Lellouch,Curtis L Cetrulo,Basak E Uygun,Martin L YarmushPubMed 35373501
- Biological & pharmaceutical bulletin 45:27-332022Coadministration of Curcumin and Hydromorphone Hydrochloride Alleviates Postoperative Pain in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYihan Wang,Yang Liu,Jieting Liu,Min Wang,Yingbin WangPubMed 34980778
- Aging 13:23004-230192021lncRNA MEG3 aggravated neuropathic pain and astrocyte overaction through mediating miR-130a-5p/CXCL12/CXCR4 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiacai Dong,Rui Xia,Zhonggui Zhang,Cheng XuPubMed 34609952
- Oncology letters 21:4442021Long non-coding RNA HOTAIR promotes the progression of synovial sarcoma through microRNA-126/stromal cell-derived factor-1 regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Feng,Donglai Wang,Peng Guo,Zibo Zhang,Jiangang FengPubMed 33868482
- Frontiers in cell and developmental biology 9:6339742021The Role of miR-31-5p in the Development of Intervertebral Disc Degeneration and Its Therapeutic Potential.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Zhou,Mingsi Deng,Jiqing Su,Wei Zhang,Dongbiao Liu,Zhengguang WangPubMed 33816484
- Journal of materials science. Materials in medicine 32:262021SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshang L Laiva,Fergal J O'Brien,Michael B KeoghPubMed 33677751
- Biomedicines 9:2021SDF-1α Gene-Activated Collagen Scaffold Restores Pro-Angiogenic Wound Healing Features in Human Diabetic Adipose-Derived Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshang L Laiva,Fergal J O'Brien,Michael B KeoghPubMed 33562165
- Biotechnology and bioengineering 118:725-7362020SDF-1α gene-activated collagen scaffold drives functional differentiation of human Schwann cells for wound healing applications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshang L Laiva,Fergal J O'Brien,Michael B KeoghPubMed 33064302
- Nature communications 11:50772020Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaohui Chen,Lijie Zhou,Lilong Liu,Yaxin Hou,Ming Xiong,Yu Yang,Junyi Hu,Ke ChenPubMed 33033240
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Predicted | Predicted |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide - Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/10000 - 1/50000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
1 product for Alternative Version
Target data
Function
Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed:29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).
Alternative names
SDF1, SDF1A, SDF1B, SDF1B, SDF1A, SDF1, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF
Recommended products
Rabbit Recombinant Monoclonal SDF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide - Human, Human samples. Cited in 22 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR1216
- Purification technique
Affinity purification Protein A
- Specificity
Mouse cross reactivity based on sequence analysis only. 100% sequence homology for isforms 1-6 (P48061; isoforms 1-6).
This antibody is not suitable for endogenous detection in Western blot application.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-SDF1 antibody [EPR1216] (ab155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--western-blot-img40889.jpg "Western blot - Anti-SDF1 antibody [EPR1216] (ab155090)")
Western blot - Anti-SDF1 antibody [EPR1216] (ab155090)
All lanes: Western blot - Anti-SDF1 antibody [EPR1216] (ab155090) at 1/10000 dilution
All lanes: Human CXCL12 recombinant protein at 0.01 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 10 kDa
![Flow Cytometry (Intracellular) - Anti-SDF1 antibody [EPR1216] (ab155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--flow-cytometry-intracellular-img138384.jpg "Flow Cytometry (Intracellular) - Anti-SDF1 antibody [EPR1216] (ab155090)")
Flow Cytometry (Intracellular) - Anti-SDF1 antibody [EPR1216] (ab155090)
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling SDF1 with purified ab155090 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
![Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--immunocytochemistry-immunofluorescence-img23943.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)")
Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)
Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling SDF1 with Purified ab155090 at 1/500 dilution (5 μg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
![Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--immunocytochemistry-immunofluorescence-img70764.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)")
Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)
Immunofluorescent analysis of Jurkat cells, labeling SDF1 with ab155090 at 1/100 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090), expandable thumbnail](https://content.abcam.com/products/images/sdf1-antibody-epr1216-ab155090--immunocytochemistry-immunofluorescence-img402159.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)")
Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC (Human primary peripheral blood mononuclear cell) cells labelling SDF1 with primary antibody anti-SDF1 (ab155090) at 1/100 dilution, followed by Alexa Fluor® 488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution. Anti-alpha Tubulin antibody (DM1A) - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue). Confocal image showing cytoplasmic staining in Human PBMC. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Low expression control: K562 (PMID: 23473997)
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