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Rabbit Multiclonal SDF1 antibody. Suitable for I-ELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CXCL12.

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Images

Indirect ELISA - Anti-SDF1 antibody [RP23040178] (AB307995), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Recombinant Fragment Protein within Human CXCL12. Database link P48061

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISA
Recombinant full length protein - Human
Tested

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1 µg/mL
Notes

-

Target data

Function

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed:29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).

Alternative names

SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF

Recommended products

Rabbit Multiclonal SDF1 antibody. Suitable for I-ELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CXCL12.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Recombinant Fragment Protein within Human CXCL12. Database link P48061
Clone number
RP23040178
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The stromal cell-derived factor 1 (SDF1) also known as C-X-C motif chemokine 12 (CXCL12) is a chemokine protein that is important in immunological responses and cellular signaling. This protein weighs approximately 8 kDa. SDF1 is largely expressed in bone marrow stroma liver and endothelium of various tissues positioning it as an integral player in cell migration and homing processes. The protein functions as a chemoattractant for lymphocytes promoting cellular trafficking and organ development.

Biological function summary

SDF1 influences the migration and survival of hematopoietic progenitor cells. It plays a pivotal role in heart development angiogenesis and neuronal protein regulation. SDF1 binds with high affinity to its receptor CXCR4 forming a critical signal transduction complex that modulates cellular movement and growth responses. This interaction is important in the regulation of cell positioning and potential pathways of pathological changes.

Pathways

SDF1 has an important role in the chemokine signaling pathway and is involved in the pathways controlling hematopoietic stem cell migration and homing. The interaction between SDF1 and CXCR4 triggers downstream signaling events engaging proteins like PI3K and MAPK which promote cell survival and proliferation. Furthermore the SDF1/CXCR4 axis is central to the vascular endothelial growth factor (VEGF) pathway facilitating angiogenesis and tissue repair mechanisms.

Associated diseases and disorders

SDF1 relates closely to cancer metastasis and HIV infection. The SDF1/CXCR4 interaction acts as a co-receptor for HIV entry into host cells implicating it in viral pathogenesis. Overexpression of SDF1 and its binding partner CXCR4 contributes to tumor growth invasion and metastasis in various cancers by promoting angiogenesis and tumor cell migration. The targeting of the SDF1/CXCR4 axis holds therapeutic potential in cancer treatment and infectious disease management.

Product promise

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1 product image

  • Indirect ELISA - Anti-SDF1 antibody [RP23040178] (ab307995), expandable thumbnail

    Indirect ELISA - Anti-SDF1 antibody [RP23040178] (ab307995)

    Indirect ELISA analysis of ab307995 was performed by coating recombinant human CXCL12 protein standard at concentrations ranging from 0.24 ng/mL to 250 ng/mL onto a plate. The coated protein was bound by ab307995 at 1 µg/mL and was detected using Goat anti-Rabbit IgG (H+L) secondary antibody, HRP-conjugate at 1/5000 dilution.

    The plate was developed using stabilized chromogen solution, TMB+H2O2 and read at 450 nm using a microplate spectrophotometer.

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Product protocols

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