AB113413

Anti-SDF4 antibody

Name: Anti-SDF4 antibody (ab113413) | Abcam
Brand: Abcam Limited
SKU: AB113413

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(1 Publication)

Goat Polyclonal SDF4 antibody. Suitable for IHC-P, WB, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SDF4 aa 150-200.

View Alternative Names

CAB45, PSEC0034, SDF4, 45 kDa calcium-binding protein, Cab45, Stromal cell-derived factor 4, SDF-4

3 Images

Flow Cytometry - Anti-SDF4 antibody (AB113413)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-SDF4 antibody (AB113413)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-SDF4 antibody (AB113413)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SDF4 antibody (AB113413)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Supplier Data

Western blot - Anti-SDF4 antibody (AB113413)

Lysates in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-SDF4 antibody (ab113413) at 0.1 µg/mL

Lane 1:

Jurkat cell lysate at 35 µg

Lane 2:

HeLa cell lysate at 35 µg

Lane 3:

U251 cell lysate at 35 µg

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human SDF4 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BRK5

Specificity

ab113413 is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab11343 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDF4 also known as Stromal Cell Derived Factor 4 or Cab45 is a calcium-binding protein with a molecular mass of approximately 45 kDa. It is expressed in the Golgi apparatus of cells in various tissues including pancreas liver and brain. SDF4 plays a mechanical role in sorting and trafficking of proteins through the secretory pathway specifically during the transport of secretory cargo in the Golgi.

Biological function summary

This protein participates in the regulation of calcium-dependent vesicle transport. It does not function as part of a large protein complex but interacts with different proteins in the Golgi for sorting activities. SDF4's ability to bind calcium allows it to modulate interactions necessary for vesicle movement and cargo recognition affecting how proteins exit the Golgi and are distributed to other areas of the cell.

Pathways

Studies show SDF4 is involved in the cellular secretory pathway and calcium signaling pathway. Within these pathways SDF4 interacts with proteins like co-chaperone SIL1 to ensure proper protein folding and trafficking. SDF4 also contributes to vesicle budding processes that are essential for maintaining proper cellular function and homeostasis.

Dysfunction of SDF4 links to disorders such as diabetes and neurodegenerative diseases. Abnormal SDF4 expression impacts proteins like insulin in the pancreatic pathway contributing to diabetes through misfolding or improper secretion of insulin. In neurodegenerative conditions SDF4's disrupted association with proteins involved in calcium homeostasis affects synaptic function and neuron health implicating it in diseases like Alzheimer's.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.. Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini.

See full target information SDF4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 12:1922-1941 PubMed35658124

2022

BCL10 Mutations Define Distinct Dependencies Guiding Precision Therapy for DLBCL.

Applications

Unspecified application

Species

Unspecified reactive species

Min Xia,Liron David,Matt Teater,Johana Gutierrez,Xiang Wang,Cem Meydan,Andrew Lytle,Graham W Slack,David W Scott,Ryan D Morin,Ozlem Onder,Kojo S J Elenitoba-Johnson,Nahuel Zamponi,Leandro Cerchietti,Tianbao Lu,Ulrike Philippar,Lorena Fontan,Hao Wu,Ari M Melnick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SDF4 antibody

Flow Cytometry - Anti-SDF4 antibody (AB113413)

Immunocytochemistry/ Immunofluorescence - Anti-SDF4 antibody (AB113413)

Western blot - Anti-SDF4 antibody (AB113413)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise