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AB137756

Anti-SDHA antibody

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(2 Publications)

Rabbit Polyclonal SDHA antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SDHA aa 1-250.

View Alternative Names

SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody (AB137756)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody (AB137756)

Immunohistochemical analysis of paraffin-embedded hepatoma tissue stained for SDHA protein with ab137756 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.

Western blot - Anti-SDHA antibody (AB137756)
  • WB

Supplier Data

Western blot - Anti-SDHA antibody (AB137756)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-SDHA antibody (ab137756) at 1/500 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Lane 3:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 73 kDa

false

Western blot - Anti-SDHA antibody (AB137756)
  • WB

Supplier Data

Western blot - Anti-SDHA antibody (AB137756)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-SDHA antibody (ab137756) at 1/500 dilution

Lane 1:

Non-transfected 293T whole cell extract at 30 µg

Lane 2:

shRNA transfected 293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 73 kDa

false

Western blot - Anti-SDHA antibody (AB137756)
  • WB

Unknown

Western blot - Anti-SDHA antibody (AB137756)

7.5% SDS PAGE

All lanes:

Western blot - Anti-SDHA antibody (ab137756) at 1/1000 dilution

All lanes:

mouse brain cell lysate at 50 µg

Predicted band size: 72 kDa

false

Western blot - Anti-SDHA antibody (AB137756)
  • WB

Supplier Data

Western blot - Anti-SDHA antibody (AB137756)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-SDHA antibody (ab137756) at 1/1000 dilution

All lanes:

Rat heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SDHA aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P31040

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.
Biological function summary

SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.

Pathways

SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.

Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 10746566, PubMed : 24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed : 20484225).
See full target information SDHA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107790 PubMed37731615

2023

Mitochondrial transplantation ameliorates doxorubicin-induced cardiac dysfunction via activating glutamine metabolism.

Applications

WB

Species

Human

Xiaolei Sun,Hang Chen,Rifeng Gao,Ya Huang,Yanan Qu,Heng Yang,Xiang Wei,Shiyu Hu,Jian Zhang,Peng Wang,Yunzeng Zou,Kai Hu,Junbo Ge,Aijun Sun

Cell reports 35:108983 PubMed33852870

2021

A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients.

Applications

Unspecified application

Species

Unspecified reactive species

Giampaolo Morciano,Gaia Pedriali,Massimo Bonora,Rita Pavasini,Elisa Mikus,Simone Calvi,Matteo Bovolenta,Magdalena Lebiedzinska-Arciszewska,Mirko Pinotti,Alberto Albertini,Mariusz R Wieckowski,Carlotta Giorgi,Roberto Ferrari,Lorenzo Galluzzi,Gianluca Campo,Paolo Pinton
View all publications
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