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Rabbit Polyclonal SDHA antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial aa 1-250.

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Images

Western blot - Anti-SDHA antibody (AB137756), expandable thumbnail
  • Western blot - Anti-SDHA antibody (AB137756), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P31040

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Cow
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:10746566, PubMed:24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed:20484225).

Alternative names

Recommended products

Rabbit Polyclonal SDHA antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P31040
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.

Biological function summary

SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.

Pathways

SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.

Associated diseases and disorders

Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.

Product promise

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2 product images

  • Western blot - Anti-SDHA antibody (ab137756), expandable thumbnail

    Western blot - Anti-SDHA antibody (ab137756)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-SDHA antibody (ab137756) at 1/1000 dilution

    All lanes: Molt4 cell lysate at 30 µg

    Predicted band size: 72 kDa

  • Western blot - Anti-SDHA antibody (ab137756), expandable thumbnail

    Western blot - Anti-SDHA antibody (ab137756)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-SDHA antibody (ab137756) at 1/1000 dilution

    All lanes: mouse brain cell lysate at 50 µg

    Predicted band size: 72 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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