Anti-SDHA antibody [HL1117]

Rabbit Recombinant Monoclonal SDHA antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Rat, Zebrafish, Mouse, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Fragment Protein within Human SDHA.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [HL1117] (AB308379), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human SDHA. The exact immunogen used to generate this antibody is proprietary information. Database link P31040

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	WB
Human	Tested	Tested	Tested
Mouse	Expected	Expected	Tested
Rat	Tested	Expected	Tested
Drosophila melanogaster	Expected	Expected	Tested
Zebrafish	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Zebrafish	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Drosophila melanogaster	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Zebrafish, Mouse, Drosophila melanogaster	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Zebrafish	Dilution info 1/1000	Notes -
Species Drosophila melanogaster	Dilution info 1/1000	Notes -

Target data

See full target information SDHA

Function

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:10746566, PubMed:24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed:20484225).

Alternative names

SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human SDHA. The exact immunogen used to generate this antibody is proprietary information. Database link P31040
Carrier free: Yes
Clone number: HL1117
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.

Biological function summary

SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.

Pathways

SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.

Associated diseases and disorders

Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [HL1117] (ab308379)
Immunohistochemical analysis of paraffin-embedded 2 days-post-fertilization zebrafish embryo tissue labelling SDHA with ab308379 at 1/50 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western blot - Anti-SDHA antibody [HL1117] (ab308379)
All lanes: Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution
All lanes: Whole S2 cell extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72 kDa
Western blot - Anti-SDHA antibody [HL1117] (ab308379)
All lanes: Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution
All lanes: Mouse heart tissue lysate at 50 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72.7 kDa
Western blot - Anti-SDHA antibody [HL1117] (ab308379)
All lanes: Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution
All lanes: Whole zebrafish lysate at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72.7 kDa
Immunohistochemistry paraffin embedded sections - Anti-SDHA antibody [HL1117] (ab308379)
Immunohistochemical analysis of human hepatocellular carcinoma tissue labeling SDHA using ab308379 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence - Anti-SDHA antibody [HL1117] (ab308379)
For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SDHA protein using ab308379 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.
Immunohistochemistry paraffin embedded sections - Anti-SDHA antibody [HL1117] (ab308379)
Immunohistochemical analysis of rat kidney tissue labeling SDHA using ab308379 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
Western blot - Anti-SDHA antibody [HL1117] (ab308379)
All lanes: Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution
All lanes: Rat heart tissue lysate at 50 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72.7 kDa
Western blot - Anti-SDHA antibody [HL1117] (ab308379)
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (ab308379) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution
Lane 1: HEK293T cell lysate at 30 µg
Lane 2: A431 cell lysate at 30 µg
Lane 3: HeLa cell lysate at 30 µg
Lane 4: HepG2 cell lysate at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72.7 kDa