AB308379

Anti-SDHA antibody [HL1117]

Recombinant
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Rabbit Recombinant Monoclonal SDHA antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Rat, Zebrafish, Mouse, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Fragment Protein within Human SDHA.

View Alternative Names

SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp

13 Images

Immunocytochemistry/ Immunofluorescence - Anti-SDHA antibody [HL1117] (AB308379)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SDHA antibody [HL1117] (AB308379)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SDHA protein using ab308379 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SDHA antibody [HL1117] (AB308379)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling SDHA protein at mitochondria using ab308379 at a 1/500 dilution (Green).
MitoTracker™ Red CMXRos, a mitochondria marker was used (Red).
DAPI was used for nuclear staining (Blue).

Immunohistochemistry paraffin embedded sections - Anti-SDHA antibody [HL1117] (AB308379)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SDHA antibody [HL1117] (AB308379)

Immunohistochemical analysis of human hepatocellular carcinoma tissue labeling SDHA using ab308379 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SDHA antibody [HL1117] (AB308379)

Immunohistochemical analysis of rat kidney tissue labeling SDHA using ab308379 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (ab308379) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution

Lane 1:

HEK293T cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Lane 3:

HeLa cell lysate at 30 µg

Lane 4:

HepG2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 72.7 kDa

false

Supplier Data

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution

Lane 1:

Jurkat whole cell lysates at 30 µg

Lane 2:

Raji whole cell lysates at 30 µg

Lane 3:

293T whole cell lysates at 30 µg

Lane 4:

A431 whole cell lysates at 30 µg

Lane 5:

HeLa whole cell lysates at 30 µg

Lane 6:

HepG2 whole cell lysates at 30 µg

Lane 7:

H1299 whole cell lysates at 30 µg

Lane 8:

HCT116 whole cell lysates at 30 µg

Lane 9:

MCF-7 whole cell lysates at 30 µg

Lane 10:

PC-3 whole cell lysates at 30 µg

Lane 11:

U87-MG whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 73 kDa

false

Supplier Data

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysates at 30 µg

Lane 2:

HepG2 mitochondria extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 73 kDa

false

Supplier Data

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution

Lane 1:

Non-transfected 293T whole cell lysates at 30 µg

Lane 2:

SDHA shRNA transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 73 kDa

false

Supplier Data

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

All lanes:

Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution

All lanes:

Mouse heart tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 72.7 kDa

false

Supplier Data

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

All lanes:

Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution

All lanes:

Rat heart tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 72.7 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [HL1117] (AB308379)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [HL1117] (AB308379)

Immunohistochemical analysis of paraffin-embedded 2 days-post-fertilization zebrafish embryo tissue labelling SDHA with ab308379 at 1/50 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

All lanes:

Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution

All lanes:

Whole S2 cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 72 kDa

false

Supplier Data

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

All lanes:

Western blot - Anti-SDHA antibody [HL1117] (ab308379) at 1/1000 dilution

All lanes:

Whole zebrafish lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 72.7 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1117

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat, Zebrafish, Drosophila melanogaster

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SDHA. The exact immunogen used to generate this antibody is proprietary information.

P31040

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.

Biological function summary

SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.

Pathways

SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.

Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 10746566, PubMed : 24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed : 20484225).

See full target information SDHA

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SDHA antibody [HL1117]

Immunocytochemistry/ Immunofluorescence - Anti-SDHA antibody [HL1117] (AB308379)

Immunocytochemistry/ Immunofluorescence - Anti-SDHA antibody [HL1117] (AB308379)

Immunohistochemistry paraffin embedded sections - Anti-SDHA antibody [HL1117] (AB308379)

Immunohistochemistry paraffin embedded sections - Anti-SDHA antibody [HL1117] (AB308379)

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [HL1117] (AB308379)

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Western blot - Anti-SDHA antibody [HL1117] (AB308379)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise