AB185222

Anti-SDHAF1 antibody [EPR13380]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal SDHAF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LYRM8, SDHAF1, SDH assembly factor 1, LYR motif-containing protein 8

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] (AB185222)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] (AB185222)

Immunofluorescent analysis of 293 cells (4% paraformaldehyde-fixed) labeling SDHAF1 with ab185222 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and Counterstained with DAPI (blue).

Lab

Western blot - Anti-SDHAF1 antibody [EPR13380] (AB185222)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SDHAF1 antibody [EPR13380] (ab185222) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysate at 20 µg

Lane 2:

MCF7 mitochondria fraction lysate at 20 µg

Lane 3:

MCF7 mitochondria control lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 13 kDa

Observed band size: 17 kDa

false

Exposure time: 3min

Carrier free

Anti-SDHAF1 antibody [EPR13380] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13380

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p>This product detects the target protein in mitochondrial lysates (17 kDa). In our hands we are unable to detect the target protein in whole cell lysates.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDHAF1 also known as Succinate Dehydrogenase Complex Assembly Factor 1 plays an important role in the assembly and maturation of succinate dehydrogenase (SDH) which is part of Complex II in the mitochondrial respiratory chain. This protein with a molecular mass of approximately 13 kDa is found primarily in the mitochondria. Its expression is necessary for the proper function of SDH which catalyzes the oxidation of succinate to fumarate in the tricarboxylic acid (TCA) cycle and participates in the electron transport chain.

Biological function summary

The interaction between SDHAF1 and other mitochondrial proteins ensures the correct assembly of the SDH complex. SDHAF1 acts as a chaperone in this process making sure that the flavoprotein subunit of SDH acquires its FAD cofactor. This assembly process is essential for maintaining cellular energy metabolism. By ensuring proper SDH complex formation SDHAF1 contributes to efficient energy production and cellular respiration.

Pathways

SDHAF1's role affects both the TCA cycle and the mitochondrial electron transport chain. The protein supports the efficient execution of these two significant pathways. Through its regulation of SDH activity SDHAF1 impacts the pathway's ability to produce ATP from the reduction of electron carriers. It interacts closely with other mitochondrial proteins involved in these pathways including SDHA SDHB SDHC and SDHD the structural subunits of SDH complex ensuring cohesive function of this energy-producing sequence.

Defective function or mutations in SDHAF1 can lead to metabolic disorders such as Leigh syndrome which is a severe neurological disorder. Mutations might impair the formation of the SDH complex disrupting the mitochondrial energy production process. Alongside SDH subunits SDHAF1 dysfunction may also contribute to disorders linked to complex II deficiencies and associated with mitochondrial myopathies. Understanding the role of SDHAF1 offers significant insight into the pathogenesis and potential treatments of these mitochondrial-related conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol (PubMed : 19465911, PubMed : 24954417). Promotes maturation of the iron-sulfur protein subunit SDHB of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants (PubMed : 24954417). May act together with SDHAF3 (PubMed : 24954417). Contributes to iron-sulfur cluster incorporation into SDHB by binding to SDHB and recruiting the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20 (PubMed : 26749241).

See full target information SDHAF1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 17:e1009539 PubMed33914734

2021

Mapping the degradation pathway of a disease-linked aspartoacylase variant.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Gersing,Yong Wang,Martin Grønbæk-Thygesen,Caroline Kampmeyer,Lene Clausen,Martin Willemoës,Claes Andréasson,Amelie Stein,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SDHAF1 antibody [EPR13380]

Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] (AB185222)

Western blot - Anti-SDHAF1 antibody [EPR13380] (AB185222)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise