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AB250820

Anti-SDHAF1 antibody [EPR13380] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SDHAF1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LYRM8, SDHAF1, SDH assembly factor 1, LYR motif-containing protein 8

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] - BSA and Azide free (AB250820)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] - BSA and Azide free (AB250820)

This data was developed using ab185222, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 293 cells (4% paraformaldehyde-fixed) labeling SDHAF1 with ab185222 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and Counterstained with DAPI (blue).

Western blot - Anti-SDHAF1 antibody [EPR13380] - BSA and Azide free (AB250820)
  • WB

Lab

Western blot - Anti-SDHAF1 antibody [EPR13380] - BSA and Azide free (AB250820)

This data was developed using ab185222, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SDHAF1 antibody [EPR13380] (<a href='/en-us/products/primary-antibodies/sdhaf1-antibody-epr13380-ab185222'>ab185222</a>) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysate at 20 µg

Lane 2:

MCF7 mitochondria fraction lysate at 20 µg

Lane 3:

MCF7 mitochondria control lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 13 kDa

Observed band size: 17 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13380

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This product detects the target protein in mitochondrial lysates (17 kDa). In our hands we are unable to detect the target protein in whole cell lysates.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250820 is the carrier-free version of ab185222.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDHAF1 also known as Succinate Dehydrogenase Complex Assembly Factor 1 plays an important role in the assembly and maturation of succinate dehydrogenase (SDH) which is part of Complex II in the mitochondrial respiratory chain. This protein with a molecular mass of approximately 13 kDa is found primarily in the mitochondria. Its expression is necessary for the proper function of SDH which catalyzes the oxidation of succinate to fumarate in the tricarboxylic acid (TCA) cycle and participates in the electron transport chain.
Biological function summary

The interaction between SDHAF1 and other mitochondrial proteins ensures the correct assembly of the SDH complex. SDHAF1 acts as a chaperone in this process making sure that the flavoprotein subunit of SDH acquires its FAD cofactor. This assembly process is essential for maintaining cellular energy metabolism. By ensuring proper SDH complex formation SDHAF1 contributes to efficient energy production and cellular respiration.

Pathways

SDHAF1's role affects both the TCA cycle and the mitochondrial electron transport chain. The protein supports the efficient execution of these two significant pathways. Through its regulation of SDH activity SDHAF1 impacts the pathway's ability to produce ATP from the reduction of electron carriers. It interacts closely with other mitochondrial proteins involved in these pathways including SDHA SDHB SDHC and SDHD the structural subunits of SDH complex ensuring cohesive function of this energy-producing sequence.

Defective function or mutations in SDHAF1 can lead to metabolic disorders such as Leigh syndrome which is a severe neurological disorder. Mutations might impair the formation of the SDH complex disrupting the mitochondrial energy production process. Alongside SDH subunits SDHAF1 dysfunction may also contribute to disorders linked to complex II deficiencies and associated with mitochondrial myopathies. Understanding the role of SDHAF1 offers significant insight into the pathogenesis and potential treatments of these mitochondrial-related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol (PubMed : 19465911, PubMed : 24954417). Promotes maturation of the iron-sulfur protein subunit SDHB of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants (PubMed : 24954417). May act together with SDHAF3 (PubMed : 24954417). Contributes to iron-sulfur cluster incorporation into SDHB by binding to SDHB and recruiting the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20 (PubMed : 26749241).
See full target information SDHAF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss
View all publications
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