Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)

Rabbit Recombinant Monoclonal SDHB antibody. Carrier free. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-SDHB antibody [EPR10880] - BSA and Azide free (AB249876), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Not recommended	Tested
Mouse	Expected	Tested	Tested	Not recommended	Expected
Rat	Expected	Tested	Tested	Not recommended	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Rat, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

See full target information SDHB

Function

Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:26925370, PubMed:27604842). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity).

Alternative names

SDH, SDH1, SDHB, Iron-sulfur subunit of complex II, Malate dehydrogenase [quinone] iron-sulfur subunit, Ip

Recommended products

Rabbit Recombinant Monoclonal SDHB antibody. Carrier free. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR10880
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab249876 is the carrier-free version of Anti-SDHB antibody [EPR10880] ab175225.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein SDHB also known as Succinate Dehydrogenase Complex Iron Sulfur Subunit B plays a mechanical role as a component of the succinate dehydrogenase (SDH) enzyme. This subunit has a molecular mass of approximately 30 kDa. SDHB is part of the mitochondrial inner membrane where it is expressed in high levels across various tissues including heart kidney and muscle. It contributes to the electron transport chain by facilitating the oxidation of succinate to fumarate an important step in energy production through the tricarboxylic acid (TCA) cycle.

Biological function summary

SDHB participates in the succinate dehydrogenase complex acting within both the TCA cycle and the electron transport chain. By transferring electrons to coenzyme Q it helps generate an electrochemical gradient important for ATP synthesis. This complex also called Complex II includes other subunits such as SDHA SDHC and SDHD. Their interactions ensure proper function of metabolic processes within mitochondria bridging the gap between foundational energy metabolism and complex cellular processes.

Pathways

SDHB plays an important role in cellular respiration and metabolic cycles. It resides in the TCA cycle and the mitochondrial electron transport chain connecting its function to energy generation pathways. Specifically SDHB relates closely to SDHA and coenzyme Q all working together to facilitate electron transfer and effective mitochondrial energy output. The transfer of electrons through this pathway highlights its essential contribution to maintaining cellular energy homeostasis.

Associated diseases and disorders

SDHB mutations are associated with conditions such as paraganglioma and pheochromocytoma. These genetic alterations disrupt normal complex II function leading to the accumulation of succinate and succinate-related oncogenesis. In addition to causing tumor development defective SDHB is linked with familial paraganglioma syndromes. Its interaction with proteins such as SDHA and SDHD critical for the succinate dehydrogenase functionality highlights its significant role in maintaining metabolic balance and when defective contributing to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Western blot - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.
Lanes 1 - 3: Merged signal (red and green). Green - Anti-SDHB antibody [EPR10880] ab175225 observed at 32 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.
Anti-SDHB antibody [EPR10880] ab175225 was shown to recognize SDHB in wild-type HEK 293 cells as signal was lost at the expected MW in SDHB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Anti-SDHB antibody [EPR10880] ab175225 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SDHB antibody [EPR10880] (Anti-SDHB antibody [EPR10880] ab175225) at 1/50000 dilution
Lane 1: Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2: SDHB knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2: Western blot - Human SDHB knockout HEK-293 cell line (Human SDHB knockout HEK-293 cell line ab260860)
Lane 3: Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 32 kDa
Western blot - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SDHB antibody [EPR10880] (Anti-SDHB antibody [EPR10880] ab175225) at 1/100000 dilution
Lane 1: HepG2 whole cell lysate at 10 µg
Lane 2: Jurkat whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SDHB with purified Anti-SDHB antibody [EPR10880] ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labeling SDHB with unpurified Anti-SDHB antibody [EPR10880] ab175225 at a dilution of 1/100.
Western blot - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SDHB antibody [EPR10880] (Anti-SDHB antibody [EPR10880] ab175225) at 1/100000 dilution
Lane 1: Mouse brain tissue lysate at 10 µg
Lane 2: Rat brain tissue lysate at 10 µg
Lane 3: Mouse spleen tissue lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human mesenchymoma tissue labelling SDHB with purified Anti-SDHB antibody [EPR10880] ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunoprecipitation - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.
Anti-SDHB antibody [EPR10880] ab175225 (purified) at a dilution of 1/60 immunoprecipitating SDHB in Jurkat whole cell lysate.
Lane 1 (input): Jurkat whole cell lysate (10μg)
Lane 2 (+): Anti-SDHB antibody [EPR10880] ab175225 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SDHB antibody [EPR10880] ab175225 in Jurkat whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-SDHB antibody [EPR10880] (Anti-SDHB antibody [EPR10880] ab175225)
Predicted band size: 32 kDa
Observed band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling SDHB with purified Anti-SDHB antibody [EPR10880] ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling SDHB with purified Anti-SDHB antibody [EPR10880] ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of A431 cells labelling SDHB with purified Anti-SDHB antibody [EPR10880] ab175225 at a dilution of 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SDHB antibody [EPR10880] (Anti-SDHB antibody [EPR10880] ab175225) at 1/50000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: A431 cell lysate at 10 µg
Lane 3: Jurkat cell lysate at 10 µg
Lane 4: Fetal heart tissue lysate at 10 µg
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling SDHB with unpurified Anti-SDHB antibody [EPR10880] ab175225 at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human carotid paraganglioma tissue labeling SDHB with unpurified Anti-SDHB antibody [EPR10880] ab175225 at a dilution of 1/100.
Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR10880] - BSA and Azide free (ab249876)
This data was developed using Anti-SDHB antibody [EPR10880] ab175225, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling SDHB with unpurified Anti-SDHB antibody [EPR10880] ab175225 at a dilution of 1/10 (red) compared to a rabbit IgG negative control (green).