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Rabbit Recombinant Monoclonal SDHB antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications.


Images

Western blot - Anti-SDHB antibody [EPR13042(B)] (AB178423), expandable thumbnail
  • Western blot - Anti-SDHB antibody [EPR13042(B)] (AB178423), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (AB178423), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (AB178423), expandable thumbnail
  • Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (AB178423), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Predicted
Not recommended
Predicted
Predicted
Predicted
Rat
Predicted
Not recommended
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:26925370, PubMed:27604842). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity).

Alternative names

SDH, SDH1, SDHB, Iron-sulfur subunit of complex II, Malate dehydrogenase [quinone] iron-sulfur subunit, Ip

Recommended products

Rabbit Recombinant Monoclonal SDHB antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13042(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein SDHB also known as Succinate Dehydrogenase Complex Iron Sulfur Subunit B plays a mechanical role as a component of the succinate dehydrogenase (SDH) enzyme. This subunit has a molecular mass of approximately 30 kDa. SDHB is part of the mitochondrial inner membrane where it is expressed in high levels across various tissues including heart kidney and muscle. It contributes to the electron transport chain by facilitating the oxidation of succinate to fumarate an important step in energy production through the tricarboxylic acid (TCA) cycle.

Biological function summary

SDHB participates in the succinate dehydrogenase complex acting within both the TCA cycle and the electron transport chain. By transferring electrons to coenzyme Q it helps generate an electrochemical gradient important for ATP synthesis. This complex also called Complex II includes other subunits such as SDHA SDHC and SDHD. Their interactions ensure proper function of metabolic processes within mitochondria bridging the gap between foundational energy metabolism and complex cellular processes.

Pathways

SDHB plays an important role in cellular respiration and metabolic cycles. It resides in the TCA cycle and the mitochondrial electron transport chain connecting its function to energy generation pathways. Specifically SDHB relates closely to SDHA and coenzyme Q all working together to facilitate electron transfer and effective mitochondrial energy output. The transfer of electrons through this pathway highlights its essential contribution to maintaining cellular energy homeostasis.

Associated diseases and disorders

SDHB mutations are associated with conditions such as paraganglioma and pheochromocytoma. These genetic alterations disrupt normal complex II function leading to the accumulation of succinate and succinate-related oncogenesis. In addition to causing tumor development defective SDHB is linked with familial paraganglioma syndromes. Its interaction with proteins such as SDHA and SDHD critical for the succinate dehydrogenase functionality highlights its significant role in maintaining metabolic balance and when defective contributing to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423), expandable thumbnail

    Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Lanes 1 - 3: Merged signal (red and green). Green - ab178423 observed at 32 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.

    ab178423 was shown to recognize SDHB in wild-type HEK 293 cells as signal was lost at the expected MW in SDHB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab178423 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/5000 dilution

    Lane 1: Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 2: SDHB knockout HEK-293 whole cell lysate at 20 µg

    Lane 2: Western blot - Human SDHB knockout HEK-293 cell line (Human SDHB knockout HEK-293 cell line ab260860)

    Lane 3: Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 32 kDa

  • Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423), expandable thumbnail

    Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    All lanes: Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/1000 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Lane 4: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SDHB with ab178423 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SDHB with ab178423 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423), expandable thumbnail

    Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Western blot analysis on immunoprecipitation pellet from (1) A431 cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178423 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Predicted band size: 32 kDa, 76 kDa

    Observed band size: 100 kDa, 85 kDa

  • Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR13042(B)] (ab178423), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling SDHB with ab178423 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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