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AB155999

Anti-SDHC antibody [EPR11035(B)]

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(16 Publications)

Rabbit Recombinant Monoclonal SDHC antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 16 publications.

View Alternative Names

CYB560, SDH3, SDHC, Integral membrane protein CII-3, Malate dehydrogenase [quinone] cytochrome b560 subunit, QPs-1, Succinate dehydrogenase complex subunit C, Succinate-ubiquinone oxidoreductase cytochrome B large subunit, QPs1, CYBL

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (AB155999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (AB155999)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling SDHC with unpurified ab155999 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (AB155999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (AB155999)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SDHC with unpurified ab155999 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (AB155999)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (AB155999)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling SDHC with purified ab155999 at 1 : 700 dilution (0.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Western blot - Anti-SDHC antibody [EPR11035(B)] (AB155999)
  • WB

Lab

Western blot - Anti-SDHC antibody [EPR11035(B)] (AB155999)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/10000 dilution

Lane 1:

THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 20 µg

Lane 2:

Human fetal liver lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Lane 4:

Rat brain lysates at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Western blot - Anti-SDHC antibody [EPR11035(B)] (AB155999)
  • WB

Lab

Western blot - Anti-SDHC antibody [EPR11035(B)] (AB155999)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/50000 dilution

All lanes:

Rat liver lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 19 kDa

false

Western blot - Anti-SDHC antibody [EPR11035(B)] (AB155999)
  • WB

Unknown

Western blot - Anti-SDHC antibody [EPR11035(B)] (AB155999)

All lanes:

Western blot - Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/10000 dilution

Lane 1:

THP-1 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

false

  • 578 PE

    PE Anti-SDHC antibody [EPR11035(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-SDHC antibody [EPR11035(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-SDHC antibody [EPR11035(B)]

  • 660 APC

    APC Anti-SDHC antibody [EPR11035(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-SDHC antibody [EPR11035(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-SDHC antibody [EPR11035(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-SDHC antibody [EPR11035(B)]

  • Carrier free

    Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11035(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/10000 - 1/50000.</strong></p><p>The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/700", "IHCP-species-notes": "<p><strong>For unpurified use at 1/250 -1/500.</strong></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/10000 - 1/50000.</strong></p><p>The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/10000 - 1/50000.</strong></p><p>The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDHC also known as succinate dehydrogenase complex subunit C or succinate dehydrogenase cytochrome b560 subunit is an integral component of the mitochondrial complex II. It has a mass of approximately 15.3 kDa. SDHC is embedded in the inner mitochondrial membrane and is expressed in various tissues with a high mitochondrial content including skeletal muscle heart and brain. Mechanically SDHC anchors the succinate dehydrogenase (SDH) complex to the mitochondrial membrane facilitating effective electron transport during cellular respiration.
Biological function summary

The subunit SDHC plays a role in the conversion of succinate to fumarate in the tricarboxylic acid (TCA) cycle. By being part of the larger succinate dehydrogenase complex which also functions as part of the electron transport chain SDHC contributes to both energy production and metabolic regulation within cells. The SDH complex consists of four subunits: SDHA SDHB SDHC and SDHD. SDHC serving as an integral membrane protein ensures the proper anchoring and assembly of the complex which is essential for efficient electron transfer.

Pathways

SDHC is involved in both the TCA cycle and the mitochondrial electron transport chain. Within these pathways it enables the transfer of electrons from succinate to ubiquinone connecting two primary bioenergetic pathways. SDHC exhibits a relationship with other complex II subunits such as SDHA which catalyzes the oxidation reaction of succinate to fumarate. The electron flow through SDHC has an impact on the entire process of oxidative phosphorylation ultimately influencing ATP production in cells.

SDHC mutations and dysfunctions have links to conditions such as paragangliomas and pheochromocytomas. These tumors are associated with altered cellular metabolism and abnormal SDH complex activity. Mutations in the SDHC gene can lead to a disruption of normal electron transport chain function causing oxidative stress and contributing to tumorigenesis. Moreover SDHC mutations often co-occur with alterations in other SDH complex components such as SDHB further emphasizing the role of this complex in certain disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 9533030). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity).
See full target information SDHC
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 12:39 PubMed39716277

2024

Similar deficiencies, different outcomes: succinate dehydrogenase loss in adrenal medulla vs. fibroblast cell culture models of paraganglioma.

Applications

Unspecified application

Species

Unspecified reactive species

Fatimah J Al Khazal,Sanjana Mahadev Bhat,Yuxiang Zhu,Cristina M de Araujo Correia,Sherry X Zhou,Brandon A Wilbanks,Clifford D Folmes,Gary C Sieck,Judith Favier,L James Maher

Cell reports 43:115038 PubMed39630581

2024

OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase.

Applications

Unspecified application

Species

Unspecified reactive species

Praveenraj Elancheliyan,Klaudia K Maruszczak,Remigiusz Adam Serwa,Till Stephan,Ahmet Sadik Gulgec,Mayra A Borrero-Landazabal,Sonia Ngati,Aleksandra Gosk,Stefan Jakobs,Michal Wasilewski,Agnieszka Chacinska

Journal of translational medicine 22:544 PubMed38844980

2024

Deficiency of SDHC promotes metastasis by reprogramming fatty acid metabolism in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoyu Ding,Yiyi Wei,Jingping Dai,Chaomin Pan,Li Yang,Qingyuan Li,Yue Zhang,Qun Yan,Changjie Wu,Aimin Li,Zhixian Lan,Side Liu,Xinke Wang

iScience 27:108883 PubMed38318358

2024

Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfan He,Gao Zhu,Xincheng Li,Mi Zhou,Min-Xin Guan

Nature 615:712-719 PubMed36922590

2023

Spatial mapping of mitochondrial networks and bioenergetics in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingqi Han,Eric A Bushong,Mayuko Segawa,Alexandre Tiard,Alex Wong,Morgan R Brady,Milica Momcilovic,Dane M Wolf,Ralph Zhang,Anton Petcherski,Matthew Madany,Shili Xu,Jason T Lee,Masha V Poyurovsky,Kellen Olszewski,Travis Holloway,Adrian Gomez,Maie St John,Steven M Dubinett,Carla M Koehler,Orian S Shirihai,Linsey Stiles,Aaron Lisberg,Stefano Soatto,Saman Sadeghi,Mark H Ellisman,David B Shackelford

Nature communications 13:6107 PubMed36245043

2022

HSF1 is a driver of leukemia stem cell self-renewal in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Qianze Dong,Yan Xiu,Yang Wang,Christina Hodgson,Nick Borcherding,Craig Jordan,Jane Buchanan,Eric Taylor,Brett Wagner,Mariah Leidinger,Carol Holman,Dennis J Thiele,Sean O'Brien,Hai-Hui Xue,Jinming Zhao,Qingchang Li,Howard Meyerson,Brendan F Boyce,Chen Zhao

Communications biology 5:453 PubMed35552531

2022

Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Takuro Toda,Mikako Ito,Jun-Ichi Takeda,Akio Masuda,Hiroyuki Mino,Nobutaka Hattori,Kaneo Mohri,Kinji Ohno

Disease markers 2022:4142684 PubMed35510040

2022

Comprehensive Analysis of Phagocytosis-Related Regulators to Aid Prognostic Prediction and Immunotherapy in Patients with Low-Grade Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwen Li,Qianrong Huang,Ligen Mo

Frontiers in cell and developmental biology 8:603688 PubMed33409279

2020

Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhua Jiang,Balaji T Moorthy,Devang M Patel,Akhilesh Kumar,William M Morgan,Belkis Alfonso,Jingyu Huang,Theodore J Lampidis,Daniel G Isom,Antoni Barrientos,Flavia Fontanesi,Fangliang Zhang

Frontiers in oncology 10:1217 PubMed32850358

2020

Mefloquine Inhibits Esophageal Squamous Cell Carcinoma Tumor Growth by Inducing Mitochondrial Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Xie,Jing Zhang,Bingbing Lu,Zhuo Bao,Jimin Zhao,Xianyu Lu,Yaxing Wei,Ke Yao,Yanan Jiang,Qiang Yuan,Xiaofan Zhang,Bo Li,Xinhuan Chen,Zigang Dong,Kangdong Liu
View all publications
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