AB191362

Anti-SDHC antibody - N-terminal

Name: Anti-SDHC antibody - N-terminal (ab191362) | Abcam
Brand: Abcam Limited
SKU: AB191362
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SDHC antibody. N-terminal. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SDHC aa 1-50.

View Alternative Names

CYB560, SDH3, SDHC, Integral membrane protein CII-3, Malate dehydrogenase [quinone] cytochrome b560 subunit, QPs-1, Succinate dehydrogenase complex subunit C, Succinate-ubiquinone oxidoreductase cytochrome B large subunit, QPs1, CYBL

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody - N-terminal (AB191362)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody - N-terminal (AB191362)

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue, labeling SDHC using ab191362 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody - N-terminal (AB191362)

Immunohistochemical analysis of paraffin-embedded rat gaster tissue, labeling SDHC using ab191362 at 1 μg/ml.

Supplier Data

Western blot - Anti-SDHC antibody - N-terminal (AB191362)

All lanes:

Western blot - Anti-SDHC antibody - N-terminal (ab191362) at 0.5 µg/mL

Lane 1:

Rat Liver Tissue Lysate at 50 µg

Lane 2:

Mouse Liver Tissue Lysate at 50 µg

Lane 3:

HeLa Whole Cell Lysate at 50 µg

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SDHC aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q99643

Specificity

ab191362 does not cross react with other proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDHC also known as succinate dehydrogenase complex subunit C or succinate dehydrogenase cytochrome b560 subunit is an integral component of the mitochondrial complex II. It has a mass of approximately 15.3 kDa. SDHC is embedded in the inner mitochondrial membrane and is expressed in various tissues with a high mitochondrial content including skeletal muscle heart and brain. Mechanically SDHC anchors the succinate dehydrogenase (SDH) complex to the mitochondrial membrane facilitating effective electron transport during cellular respiration.

Biological function summary

The subunit SDHC plays a role in the conversion of succinate to fumarate in the tricarboxylic acid (TCA) cycle. By being part of the larger succinate dehydrogenase complex which also functions as part of the electron transport chain SDHC contributes to both energy production and metabolic regulation within cells. The SDH complex consists of four subunits: SDHA SDHB SDHC and SDHD. SDHC serving as an integral membrane protein ensures the proper anchoring and assembly of the complex which is essential for efficient electron transfer.

Pathways

SDHC is involved in both the TCA cycle and the mitochondrial electron transport chain. Within these pathways it enables the transfer of electrons from succinate to ubiquinone connecting two primary bioenergetic pathways. SDHC exhibits a relationship with other complex II subunits such as SDHA which catalyzes the oxidation reaction of succinate to fumarate. The electron flow through SDHC has an impact on the entire process of oxidative phosphorylation ultimately influencing ATP production in cells.

SDHC mutations and dysfunctions have links to conditions such as paragangliomas and pheochromocytomas. These tumors are associated with altered cellular metabolism and abnormal SDH complex activity. Mutations in the SDHC gene can lead to a disruption of normal electron transport chain function causing oxidative stress and contributing to tumorigenesis. Moreover SDHC mutations often co-occur with alterations in other SDH complex components such as SDHB further emphasizing the role of this complex in certain disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 9533030). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity).

See full target information SDHC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 6:375 PubMed34728602

2021

The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.

Applications

Unspecified application

Species

Unspecified reactive species

Xujun Liu,Wenzhe Si,Lin He,Jianguo Yang,Yani Peng,Jie Ren,Xiaoping Liu,Tong Jin,Huajing Yu,Zihan Zhang,Xiao Cheng,Wenting Zhang,Lu Xia,Yunchao Huang,Yue Wang,Shumeng Liu,Lin Shan,Yu Zhang,Xiaohan Yang,Haixia Li,Jing Liang,Luyang Sun,Yongfeng Shang

Journal of biological methods 5: PubMed29862308

2018

Insulin receptor based lymphocyte trafficking in the progression of type 1 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Michael P Morran,Ali G Al-Dieri,Andrea L Nestor-Kalinoski,Richard K Jordan,Nirdesh K Gupta,Marcia F McInerney

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SDHC antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody - N-terminal (AB191362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody - N-terminal (AB191362)

Western blot - Anti-SDHC antibody - N-terminal (AB191362)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise