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AB189945

Anti-SDHD antibody

5

(1 Review)

|

(11 Publications)

Rabbit Polyclonal SDHD antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SDHD.

View Alternative Names

SDH4, SDHD, CybS, CII-4, Malate dehydrogenase [quinone] cytochrome b small subunit, QPs3, Succinate dehydrogenase complex subunit D, Succinate-ubiquinone oxidoreductase cytochrome b small subunit, Succinate-ubiquinone reductase membrane anchor subunit

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHD antibody (AB189945)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHD antibody (AB189945)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling SDHD using ab189945 at 2.5 μg/ml.

Western blot - Anti-SDHD antibody (AB189945)
  • WB

Supplier Data

Western blot - Anti-SDHD antibody (AB189945)

Lane 1:

Western blot - Anti-SDHD antibody (ab189945) at 1 µg/mL

Lane 2:

Western blot - Anti-SDHD antibody (ab189945) at 2 µg/mL

All lanes:

mouse EL4 cell lysate

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SDHD. The exact immunogen used to generate this antibody is proprietary information.

O14521

Specificity

ab189945 will detect three isoforms of SDHD.

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Alternative Products

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AB203199

Anti-SDHD antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SDHD protein also known as succinate dehydrogenase complex subunit D serves a function in cellular respiration. This protein is a part of the succinate dehydrogenase (SDH) complex found in the inner mitochondrial membrane. This complex is known as complex II in the electron transport chain. The subunit D with a mass of about 15 kDa anchors the larger SDH complex to the membrane and is essential for its structural stability. SDHD is widely expressed in tissues with high energy demands such as the heart liver and muscles.
Biological function summary

The SDH complex has an essential role in both the Krebs cycle and the mitochondrial electron transport chain. SDHD as part of this complex assists in the oxidation of succinate to fumarate an important step in the Krebs cycle. The electrons generated from succinate oxidation are transferred through the SDH complex to ubiquinone contributing to ATP production. The complex facilitates the coupling of the Krebs cycle to the electron transport chain highlighting SDHD’s importance in efficient energy metabolism.

Pathways

SDHD integrates into the Krebs cycle and the electron transport chain linking these vital energy-yielding reactions. In the Krebs cycle it assists in converting succinate to fumarate contributing important intermediates and electron donors for oxidative phosphorylation. It interacts with other components of complex II such as SDHA SDHB and SDHC to facilitate its enzymatic functions. Furthermore its role in electron transport involves ubiquinone which carries electrons to complex III continuing the chain of reactions needed for energy production.

SDHD mutations have been linked with paragangliomas and pheochromocytomas both of which are neuroendocrine tumors. These conditions are associated with disrupted cellular energy metabolism caused by impaired function of the SDH complex. In these tumors mutations in SDHD can lead to a pseudohypoxic state promoting cell proliferation. The protein's dysfunction connects pathophysiologically to related SDHB SDHC and SDHA subunits with mutations in these subunits also contributing to the manifestation of such tumors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 10482792, PubMed : 9533030). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity).
See full target information SDHD
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cell biochemistry and biophysics 83:3341-3351 PubMed40072831

2025

sPLA2-IB and PLA2R Mediate Aberrant Glucose Metabolism in Podocytes via Hyperactivation of the mTOR/HIF-1α Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwen Bao,Binbin Dai,Liyan Yang,Zikang Liu,Yuxuan Jin,Hanxue Zhao,Yangbin Pan

Frontiers in immunology 14:1197773 PubMed37790931

2023

Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yunlong Zhang,Yue Ma,Qingqing Liu,Yifei Du,Liang Peng,Jianwu Zhou,Zhenzhen Zhao,Changchun Li,Shan Wang

iScience 26:107790 PubMed37731615

2023

Mitochondrial transplantation ameliorates doxorubicin-induced cardiac dysfunction via activating glutamine metabolism.

Applications

WB

Species

Human

Xiaolei Sun,Hang Chen,Rifeng Gao,Ya Huang,Yanan Qu,Heng Yang,Xiang Wei,Shiyu Hu,Jian Zhang,Peng Wang,Yunzeng Zou,Kai Hu,Junbo Ge,Aijun Sun

Nature communications 13:6107 PubMed36245043

2022

HSF1 is a driver of leukemia stem cell self-renewal in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Qianze Dong,Yan Xiu,Yang Wang,Christina Hodgson,Nick Borcherding,Craig Jordan,Jane Buchanan,Eric Taylor,Brett Wagner,Mariah Leidinger,Carol Holman,Dennis J Thiele,Sean O'Brien,Hai-Hui Xue,Jinming Zhao,Qingchang Li,Howard Meyerson,Brendan F Boyce,Chen Zhao

Communications biology 5:453 PubMed35552531

2022

Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Takuro Toda,Mikako Ito,Jun-Ichi Takeda,Akio Masuda,Hiroyuki Mino,Nobutaka Hattori,Kaneo Mohri,Kinji Ohno

BMC cancer 21:1200 PubMed34763667

2021

TGF-β protects osteosarcoma cells from chemotherapeutic cytotoxicity in a SDH/HIF1α dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yangbo Xu,Yafei Li,Xiaofan Chen,Feifan Xiang,Yong Deng,Zhong Li,Daiqing Wei

Genes 12: PubMed34828261

2021

A Quantitative Proteomics Approach to Gain Insight into NRF2-KEAP1 Skeletal Muscle System and Its Cysteine Redox Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Rafay Abu,Li Yu,Ashok Kumar,Lie Gao,Vikas Kumar

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34551979

2021

Functional succinate dehydrogenase deficiency is a common adverse feature of clear cell renal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ritesh K Aggarwal,Rebecca A Luchtel,Venkata Machha,Alexander Tischer,Yiyu Zou,Kith Pradhan,Nadia Ashai,Nandini Ramachandra,Joseph M Albanese,Jung-In Yang,Xiaoyang Wang,Srinivas Aluri,Shanisha Gordon,Ahmed Aboumohamed,Benjamin A Gartrell,Sassan Hafizi,James Pullman,Niraj Shenoy

Frontiers in oncology 10:1217 PubMed32850358

2020

Mefloquine Inhibits Esophageal Squamous Cell Carcinoma Tumor Growth by Inducing Mitochondrial Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Xie,Jing Zhang,Bingbing Lu,Zhuo Bao,Jimin Zhao,Xianyu Lu,Yaxing Wei,Ke Yao,Yanan Jiang,Qiang Yuan,Xiaofan Zhang,Bo Li,Xinhuan Chen,Zigang Dong,Kangdong Liu

Cancer research 77:1649-1661 PubMed28108517

2017

Promoter Mutations Ablate GABP Transcription Factor Binding in Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tongwu Zhang,Mai Xu,Matthew M Makowski,Christine Lee,Michael Kovacs,Jun Fang,Esther Willems,Jeffrey M Trent,Nicholas K Hayward,Michiel Vermeulen,Kevin M Brown
View all publications
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Product promise

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