JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB68536

Anti-SDS antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Polyclonal SDS antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SDS.

View Alternative Names

SDH, SDS, L-serine dehydratase/L-threonine deaminase, Hepatic serine dehydratase, L-serine deaminase, L-threonine dehydratase, hSDH, TDH

2 Images
Western blot - Anti-SDS antibody (AB68536)
  • WB

Unknown

Western blot - Anti-SDS antibody (AB68536)

All lanes:

Western blot - Anti-SDS antibody (ab68536) at 1/500 dilution

All lanes:

Human liver lysate at 50 µg

Secondary

All lanes:

Goat anti-mouse (H+L) IgG at 1/2500 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Western blot - Anti-SDS antibody (AB68536)
  • WB

Unknown

Western blot - Anti-SDS antibody (AB68536)

All lanes:

Western blot - Anti-SDS antibody (ab68536) at 1/500 dilution

Lane 1:

SDS transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T lysate. at 25 µg

Secondary

All lanes:

Goat anti-mouse (H+L) at 1/2500 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SDS.

P20132

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sodium dodecyl sulfate (SDS) also known as sodium lauryl sulfate is an anionic detergent with a molecular weight of 288.38 g/mol. It disrupts non-covalent bonds in proteins causing them to denature. SDS is widely used for its ability to solubilize proteins by coating them in negative charge ensuring uniform migration during electrophoresis. In laboratory settings SDS is most often expressed in solutions prepared for SDS-PAGE a common technique to separate proteins based on molecular weight.
Biological function summary

Scientists employ sodium dodecyl sulfate in the analysis of protein composition and structure. SDS facilitates the study of protein complexes by breaking apart aggregates into individual polypeptide chains. This function is particularly important in research aiming to determine protein characteristics such as size purity and content. Researchers often pair SDS with reducing agents to further break disulfide bonds in proteins offering clearer insights into the proteins' linear arrangement.

Pathways

The use of sodium dodecyl sulfate is important for revealing information underpinned by protein characterization and separation protocols. In processes like the MAPK/ERK signaling pathway understanding individual protein constituents is important. Additionally related proteins such as kinases and phosphatases have their activities scrutinized through SDS-PAGE analysis enabling better assessment of their roles in signaling pathways.

The accuracy of sodium dodecyl sulfate in protein study helps in understanding conditions such as Alzheimer's disease and some cancers. Scientists analyze variations in protein expression and aggregation considering proteins like amyloid-beta and oncogenes in these disorders. SDS makes it possible to detect these alterations early on which helps in advancing diagnostic and therapeutic strategies against such conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the pyridoxal-phosphate-dependent dehydrative deamination of L-threonine and L-serine to ammonia and alpha-ketobutyrate and pyruvate, respectively.
See full target information SDS
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 29:3331-3348.e7 PubMed31801093

2019

Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin D Stein,Diego Calzolari,Kristina Hellberg,Ying S Hu,Lin He,Chien-Min Hung,Erin Q Toyama,Debbie S Ross,Björn F Lillemeier,Lewis C Cantley,John R Yates,Reuben J Shaw

Amino acids 39:145-53 PubMed19921396

2009

The effects of a low protein diet on amino acids and enzymes in the serine synthesis pathway in mice.

Applications

WB

Species

Mouse

Jordan E Antflick,Glen B Baker,David Richard Hampson
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB306577

Anti-PSPH antibody [EPR26995-88A]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] (AB306577)

  • 0
  • 0
Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
  • 6
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 19

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com