AB250757

Anti-SDS3 antibody [EPR15000] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SDS3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SAP45, SDS3, SUDS3, Sin3 histone deacetylase corepressor complex component SDS3, 45 kDa Sin3-associated polypeptide, Suppressor of defective silencing 3 protein homolog

2 Images

Flow Cytometry (Intracellular) - Anti-SDS3 antibody [EPR15000] - BSA and Azide free (AB250757)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SDS3 antibody [EPR15000] - BSA and Azide free (AB250757)

This data was developed using ab184555, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SDS3 with ab184555 at 1/250 dilution (red) compared to a Rabbit monoclonal isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Western blot - Anti-SDS3 antibody [EPR15000] - BSA and Azide free (AB250757)

This data was developed using ab184555, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SDS3 antibody [EPR15000] (<a href='/en-us/products/primary-antibodies/sds3-antibody-epr15000-ab184555'>ab184555</a>) at 1/1000 dilution

Lane 1:

Raji cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Molt-4 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 38 kDa

Observed band size: 45 kDa

false

Unconjugated

Anti-SDS3 antibody [EPR15000]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15000

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250757 is the carrier-free version of ab184555.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDS3 also known as SAP30 binding protein (SAP30BP) is a protein with a molecular weight of about 46 kDa. This protein is expressed in various tissues notably in the nucleus of cells. Mechanically SDS3 functions as a transcriptional regulator playing an essential role in chromatin remodeling and gene expression. It is a part of histone deacetylase complexes where it helps modulate the structural conformation of chromatin affecting DNA accessibility.

Biological function summary

SDS3 exerts important roles by being a component of the Sin3a corepressor complex. This complex is responsible for the repression of transcription through histone modification specifically deacetylation which leads to chromatin condensation. In this way SDS3 contributes to silencing specific genes which can influence cell differentiation and proliferation. Its interactions within the complex contribute to the regulation of diverse genes vital for cellular homeostasis.

Pathways

SDS3's interactions intervene in the transcriptional repression pathways and cell cycle regulation pathways. Involvement in these pathways showcases its role in maintaining proper cell division and controlling gene expression timing. SDS3 is closely related to other proteins like histone deacetylases HDAC1 and HDAC2 which participate in deacetylation activities that repress transcription and modify chromatin structure to restrict gene accessibility.

SDS3 has been linked to various cancers due to its role in regulating genes that control cell proliferation and survival. Abnormal expression or function of SDS3 can lead to inappropriate transcriptional repression of tumor suppressor genes contributing to unchecked cell growth. Additionally SDS3 is associated with neurodegenerative disorders due to its role in gene expression regulation in neural cells. In these conditions SDS3 may interact with other proteins such as SIN3A and RBBP4 further impacting gene expression profiles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways through regulation of apoptosis. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.

See full target information SUDS3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SDS3 antibody [EPR15000] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-SDS3 antibody [EPR15000] - BSA and Azide free (AB250757)

Western blot - Anti-SDS3 antibody [EPR15000] - BSA and Azide free (AB250757)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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