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AB250083

Anti-SDSL antibody [EPR12192] - BSA and Azide free

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Rabbit Recombinant Monoclonal SDSL antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Serine dehydratase-like, Cancerous serine dehydratase, Glutamate racemase, L-serine deaminase, L-serine dehydratase/L-threonine deaminase, L-threonine dehydratase, Serine dehydratase 2, cSDH, SDHL, TDH, SDH 2, SDSL

1 Images
Western blot - Anti-SDSL antibody [EPR12192] - BSA and Azide free (AB250083)
  • WB

Supplier Data

Western blot - Anti-SDSL antibody [EPR12192] - BSA and Azide free (AB250083)

This data was developed using ab179435, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SDSL antibody [EPR12192] (<a href='/en-us/products/primary-antibodies/sdsl-antibody-epr12192-ab179435'>ab179435</a>) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

Caco-2 lysate at 10 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12192

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250083 is the carrier-free version of ab179435.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The serine dehydratase-like protein (SDSL) also known by its alternate name as serine dehydratase-like 2 exhibits a molecular mass of approximately 42 kDa. SDSL functions mechanically by catalyzing the dehydration reaction of L-serine to form pyruvate and ammonia. This protein is expressed predominantly in the liver with detectable expression in other tissues including the kidney and intestine. Researchers have noted that SDSL might play a role in amino acid metabolism due to its catalytic activity.
Biological function summary

The serine dehydratase-like protein contributes to the metabolism of amino acids an essential process for cellular function and energy production. SDSL does not appear to form part of a larger protein complex but works independently in biochemical pathways related to the breakdown of serine. This activity is significant for maintaining amino acid homeostasis within the cell and supporting gluconeogenesis.

Pathways

Amino acid metabolism and gluconeogenesis represent major biological pathways involving SDSL. In the amino acid metabolism pathway SDSL interacts with enzymes like alanine transaminase and aspartate aminotransferase which further process the products of the dehydratase reaction. In gluconeogenesis the pyruvate produced serves as a substrate for enzymes like pyruvate carboxylase illustrating how SDSL indirectly supports glucose synthesis during fasting or low-carbohydrate intake.

Disturbances in serine metabolism where SDSL is an active participant have connections to disorders such as non-alcoholic fatty liver disease (NAFLD) and phenylketonuria (PKU). In NAFLD an overexpression or aberrant activity of SDSL could contribute to altered amino acid handling influencing liver fat accumulation processes. In PKU connections with phenylalanine hydroxylase show that altered serine metabolism might impact phenylalanine processing indicating a potential area for therapeutic exploration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the pyridoxal-phosphate-dependent dehydrative deamination of L-threonine and L-serine to ammonia and alpha-ketobutyrate and pyruvate, respectively (PubMed : 16580895, PubMed : 18342636). Also exhibits racemase activity towards L-glutamate and D-glutamate (By similarity).
See full target information SDSL
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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