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AB91645

Anti-Seasonal and Swine H1N1 Neuraminidase antibody

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(1 Publication)

Rabbit Polyclonal Seasonal and Swine H1N1 Neuraminidase antibody. Suitable for ELISA, WB and reacts with Synthetic peptide - Influenza A virus (A/Georgia/20/2006(H1N1)), Synthetic peptide - Influenza A virus (A/California/14/2009(H1N1)), Recombinant full length protein - Influenza A virus (A/California/14/2009(H1N1)) samples. Cited in 1 publication.
3 Images
ELISA - Anti-Seasonal and Swine H1N1 Neuraminidase antibody (AB91645)
  • ELISA

Unknown

ELISA - Anti-Seasonal and Swine H1N1 Neuraminidase antibody (AB91645)

ELISA using ab91645 at 1 μg/ml, detects 2 ng of free peptide (Seasonal and Swine H1N1 Neuraminidase).

ELISA - Anti-Seasonal and Swine H1N1 Neuraminidase antibody (AB91645)
  • ELISA

Supplier Data

ELISA - Anti-Seasonal and Swine H1N1 Neuraminidase antibody (AB91645)

Validation with H1N1 NA Protein Coating Antigen : H1N1 Neuraminidase recombinant protein, 2 μg/mL, incubated at 4˚C overnight. Detection Antibodies : ab91645, dilution : 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies : Goat anti-rabbit at 1/10000 dilution, incubated at RT for 1 hr.

Western blot - Anti-Seasonal and Swine H1N1 Neuraminidase antibody (AB91645)
  • WB

Supplier Data

Western blot - Anti-Seasonal and Swine H1N1 Neuraminidase antibody (AB91645)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Seasonal and Swine H1N1 Neuraminidase antibody (ab91645) at 2 µg/mL

Lane 1:

H1N1 Swine Neuraminidase recombinant protein at 50 ng

Lane 2:

H1N1 Swine Neuraminidase recombinant protein at 100 ng

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Influenza A virus (A/Georgia/20/2006(H1N1)), Influenza A virus (A/California/14/2009(H1N1))

Applications

WB, ELISA

applications

Specificity

Recognizes the Neuraminidase from the both the Seasonal H1N1 Neuraminidase and Swine H1N1 Neuraminidase (A/California/14/2009 (H1N1)) in ELISA and WB. It will detect 2 ng of free peptide at 1 μg/mL.

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Complementary Products

Primary Antibodies

AB91646

Anti-Influenza A H1N1 Neuraminidase antibody

ELISA - Anti-Influenza A H1N1 Neuraminidase antibody (AB91646)

  • 0
  • 0
Primary Antibodies

AB197020

Anti-Neuraminidase antibody [EPR15712]

RabMAb|Recombinant

Western blot - Anti-Neuraminidase antibody [EPR15712] (AB197020)

  • 0
  • 0
Primary Antibodies

AB21304

Anti-Avian Influenza A Neuraminidase antibody

ELISA - Anti-Avian Influenza A Neuraminidase antibody (AB21304)

  • 2
  • 1
Primary Antibodies

AB91643

Anti-Swine H1N1 Neuraminidase antibody

ELISA - Anti-Swine H1N1 Neuraminidase antibody (AB91643)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A virus (A/California/14/2009(H1N1))": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Influenza A virus (A/Georgia/20/2006(H1N1))": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Influenza A virus (A/California/14/2009(H1N1))": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" }, "Synthetic peptide - Influenza A virus (A/California/14/2009(H1N1))": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide - Influenza A virus (A/Georgia/20/2006(H1N1))": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as Seasonal and Swine H1N1 Neuraminidase often referred to simply as neuraminidase plays a significant mechanical role in the influenza virus life cycle. Neuraminidase is an integral membrane protein with a molecular mass that varies between different viral strains but is typically around 60 kDa. It is expressed on the surface of influenza viral particles where it facilitates viral release from infected host cells by cleaving sialic acid residues from glycoproteins and glycolipids. This enzymatic action enables mature virions to detach from the host cell promoting further spread of the infection to neighboring cells.
Biological function summary

The neuraminidase protein acts as a sialidase catalyzing the hydrolysis of terminal sialic acid residues. This protein functions as part of a tetramer complex on the viral envelope where its activity complements that of hemagglutinin another surface protein. While hemagglutinin allows the virus to attach to the host cell by binding to sialic acid neuraminidase enables the virus to release and spread by cleaving these same residues post-replication. This balance between attachment and release is important for optimal viral propagation and infection cycle continuation.

Pathways

Neuraminidase activity intersects in the host cell pathways involved in glycan modification and processing. Neuraminidase contributes to the process known as glycan degradation where the removal of sialic acid groups modulates cell-surface molecule interactions. This protein and its functional counterpart hemagglutinin are central to the viral replication pathway and the overall influenza infection pathway. Both proteins coordinate their activities to ensure efficient viral entry and exit from host cells underpinning the infectious cycle of the virus.

Neuraminidase has a critical role in influenza infections such as seasonal flu and swine flu (H1N1). Inhibitors targeting neuraminidase are key therapeutic agents aimed at reducing viral load by effectively preventing the release of new viral particles. Oseltamivir and zanamivir serve as examples of neuraminidase inhibitors used in treating influenza. Neuraminidase connects directly with hemagglutinin during the infection process forming a target for vaccine development and antiviral drug design to combat influenza epidemics and pandemics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Seasonal and Swine H1N1 Neuraminidase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 20:183 PubMed37596622

2023

Influenza a virus regulates interferon signaling and its associated genes; MxA and STAT3 by cellular miR-141 to ensure viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Mai Alalem,Emad Dabous,Ahmed M Awad,Nedaa Alalem,Adel A Guirgis,Samir El-Masry,Hany Khalil
View all publications
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Product promise

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