Anti-SEC10 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-SEC10 antibody (AB241472)

SEC10 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241472 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241472 at 1 μg/ml.

Lane 1 : ab241472 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-SEC10 antibody (ab241472)

Predicted band size: 82 kDa

false

• WB

Supplier Data

Western blot - Anti-SEC10 antibody (AB241472)

Lysates prepared in NETN lysis buffer.

All lanes:

Western blot - Anti-SEC10 antibody (ab241472) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 82 kDa

true

Exposure time: 3min