AB168824

Anti-SEC13L1 antibody

Name: Anti-SEC13L1 antibody (ab168824) | Abcam
Brand: Abcam Limited
SKU: AB168824
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal SEC13L1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SEC13 aa 250-350.

View Alternative Names

D3S1231E, SEC13A, SEC13L1, SEC13R, SEC13, Protein SEC13 homolog, GATOR2 complex protein SEC13, SEC13-like protein 1, SEC13-related protein

2 Images

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Immunoprecipitation - Anti-SEC13L1 antibody (AB168824)

Detection of SEC13L1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168824 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SEC13L1 antibody (ab168824)

Predicted band size: 35 kDa

false

Unknown

Western blot - Anti-SEC13L1 antibody (AB168824)

All lanes:

Western blot - Anti-SEC13L1 antibody (ab168824) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 35 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human SEC13 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P55735

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab168824 was affinity purified using an epitope specific to SEC13L1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC13L1 also known as SEC13 or SEC13-like is a protein encoded by the SEC13L1 gene. It is a component of a complex involved in vesicle trafficking. The protein has an approximate molecular mass of 37 kDa and is known to be expressed in various tissues across the human body indicating a broad functional role. SEC13L1 is an integral part of the coated vesicles that mediate protein transportation between the endoplasmic reticulum and the Golgi apparatus.

Biological function summary

As a part of the SEC13 complex SEC13L1 serves an essential function in the process of vesicular transport. This protein forms a core element of the COPII coat complex which is a multi-protein assembly responsible for the budding of transport vesicles from the endoplasmic reticulum. Besides its role in protein transport SEC13L1 also participates in maintaining the architecture of the nuclear pore complex which is critical for nucleocytoplasmic transport.

Pathways

SEC13L1 plays an important role in the secretory pathway and is involved in the ER-Golgi transport pathway. It associates closely with proteins like SEC24 and SEC23 which work together in the COPII coat formation. The interaction with these proteins is important for the selection of cargo proteins that are exported in COPII vesicles. Furthermore SEC13L1 is also involved in the regulation of nucleocytoplasmic transport pathways alongside proteins such as NUP107 as part of the nuclear pore complex degradation.

SEC13L1 has been associated with nephrotic syndrome where its dysregulation may impact protein trafficking and cellular homeostasis. It also plays a role in cancer where the protein's involvement in nuclear pore functions and vesicle formation may affect cell proliferation and survival. SEC13L1 links to disorders involving nucleocytoplasmic transport often interacting with nucleoporins such as NUP133 that may be implicated in pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a component of the nuclear pore complex (NPC) and the COPII coat (PubMed : 8972206). At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles (PubMed : 8972206). Required for the exit of adipsin (CFD/ADN), an adipocyte-secreted protein from the endoplasmic reticulum (By similarity).. As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway (PubMed : 23723238, PubMed : 25457612, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex (PubMed : 23723238, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1 (PubMed : 36528027). In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation (PubMed : 36528027). In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex (PubMed : 25457612, PubMed : 26972053, PubMed : 27487210). Within the GATOR2 complex, SEC13 and SEH1L are required to stabilize the complex (PubMed : 35831510).

See full target information SEC13

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of medical virology 96:e70116 PubMed39704187

2024

Long Noncoding RNA LINC02453 Inhibits HIV-1 Replication by Binding With SEC13 to Regulate the Viral Productive Cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu Chen,Rongfeng Chen,Liufang Wen,Tongxue Qin,Yinlu Liao,Xing Tao,Zongxiang Yuan,Wudi Wei,Jinmiao Li,Youjin Huang,Wenfei Wei,Jie Liu,Jinming Su,Li Ye,Hao Liang,Junjun Jiang

iScience 27:109180 PubMed38439956

2024

Mea6/cTAGE5 cooperates with TRAPPC12 to regulate PTN secretion and white matter development.

Applications

Unspecified application

Species

Unspecified reactive species

Tiantian Ma,Yaqing Wang,Laikang Yu,Jinghua Liu,Tao Wang,Pengyu Sun,Yinghang Feng,Dan Zhang,Lei Shi,Kangmin He,Li Zhao,Zhiheng Xu

EMBO reports 21:e49898 PubMed32648345

2020

Aster-C coordinates with COP I vesicles to regulate lysosomal trafficking and activation of mTORC1.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhang,John-Paul Andersen,Haoran Sun,Xuyun Liu,Nahum Sonenberg,Jia Nie,Yuguang Shi

The Journal of cell biology 216:4153-4164 PubMed29133483

2017

deletion in pancreatic β cells impairs proinsulin trafficking and insulin biogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Junwan Fan,Yaqing Wang,Liang Liu,Hongsheng Zhang,Feng Zhang,Lei Shi,Mei Yu,Fei Gao,Zhiheng Xu

View all publications

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Product promise

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Anti-SEC13L1 antibody

Immunoprecipitation - Anti-SEC13L1 antibody (AB168824)

Western blot - Anti-SEC13L1 antibody (AB168824)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise