Rabbit Recombinant Monoclonal SEC14L2/TAP antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (AB248765), expandable thumbnail](https://content.abcam.com/products/images/sec14l2-tap-antibody-epr8612-bsa-and-azide-free-ab248765--western-blot-img111973.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (AB248765)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (AB248765), expandable thumbnail](https://content.abcam.com/products/images/sec14l2-tap-antibody-epr8612-bsa-and-azide-free-ab248765--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95628.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (AB248765)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (AB248765), expandable thumbnail](https://content.abcam.com/products/images/sec14l2-tap-antibody-epr8612-bsa-and-azide-free-ab248765--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117950.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (AB248765)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Carrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell.
Alternative names
C22orf6, KIAA1186, KIAA1658, SEC14L2, SEC14-like protein 2, Alpha-tocopherol-associated protein, Squalene transfer protein, Supernatant protein factor, TAP, hTAP, SPF
Recommended products
Rabbit Recombinant Monoclonal SEC14L2/TAP antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8612
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248765 is the carrier-free version of Anti-SEC14L2/TAP antibody [EPR8612] ab137013.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEC14L2 also known as TAP is a member of the SEC14-like family of proteins. This protein has a molecular mass of about 44 kDa. It plays an important role in lipid transport and metabolism by acting as a phosphatidylinositol transfer protein. SEC14L2 is expressed in a variety of tissues with higher levels observed in the liver. It functions by transferring phospholipids between membranes which is essential for maintaining cellular lipid homeostasis.
- Biological function summary
The SEC14L2 protein influences several important cellular processes such as vesicular trafficking and signal transduction. It is capable of interacting with lipid molecules to modulate their bioavailability and distribution within cells. SEC14L2 does not function alone; it forms interactions with other proteins to fulfill its role though the specific complexes it involves remain a subject of research. This protein is important for the regulation of lipid signaling pathways linking it directly to cellular function and metabolism.
- Pathways
SEC14L2 engages in lipid signaling and metabolic pathways. It is integral to maintaining phosphatidylinositol signaling a critical pathway in cellular communication. SEC14L2 interacts with other proteins involved in lipid homeostasis such as PI3K which plays a role in various signaling cascades that regulate growth and metabolism. By influencing these pathways SEC14L2 affects cell proliferation and survival establishing its importance in cell cycle regulation and metabolism.
- Associated diseases and disorders
Alterations in SEC14L2 expression or function have connections with liver cancer and metabolic syndromes. Misregulation of this protein might contribute to aberrant lipid metabolism that leads to oncogenesis highlighting its involvement in cancer biology. SEC14L2 has possible interactions with other proteins like PPARs which are important in metabolic disorders. By exploring these associations researchers gain insights into the potential therapeutic targets for diseases linked to metabolic dysregulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (ab248765), expandable thumbnail](https://content.abcam.com/products/images/sec14l2-tap-antibody-epr8612-bsa-and-azide-free-ab248765--western-blot-img111973.jpg "Western blot - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (ab248765)")
Western blot - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (ab248765)
This data was developed using Anti-SEC14L2/TAP antibody [EPR8612] ab137013, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SEC14L2/TAP antibody [EPR8612] (Anti-SEC14L2/TAP antibody [EPR8612] ab137013) at 1/1000 dilution
Lane 1: Fetal liver tissue lysate at 10 µg
Lane 2: Fetal brain tissue lysate at 10 µg
Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg
Lane 4: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (ab248765), expandable thumbnail](https://content.abcam.com/products/images/sec14l2-tap-antibody-epr8612-bsa-and-azide-free-ab248765--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95628.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (ab248765)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (ab248765)
This data was developed using Anti-SEC14L2/TAP antibody [EPR8612] ab137013, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling SEC14L2/TAP with Anti-SEC14L2/TAP antibody [EPR8612] ab137013 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (ab248765), expandable thumbnail](https://content.abcam.com/products/images/sec14l2-tap-antibody-epr8612-bsa-and-azide-free-ab248765--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117950.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (ab248765)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] - BSA and Azide free (ab248765)
This data was developed using Anti-SEC14L2/TAP antibody [EPR8612] ab137013, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling SEC14L2/TAP with Anti-SEC14L2/TAP antibody [EPR8612] ab137013 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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