AB70722

Anti-SEC16A/SEC16 antibody

Name: Anti-SEC16A/SEC16 antibody (ab70722) | Abcam
Brand: Abcam Limited
SKU: AB70722
Price: 535 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(6 Publications)

Rabbit Polyclonal SEC16A/SEC16 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SEC16A.

View Alternative Names

KIAA0310, SEC16, SEC16L, SEC16A, Protein transport protein Sec16A, SEC16 homolog A, p250

2 Images

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Immunoprecipitation - Anti-SEC16A/SEC16 antibody (AB70722)

Detection of Human SEC16A/SEC16 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70722 at 3 μg/mg lysate. Subsequent Western blot detection of SEC16A/SEC16 was performed using ab70722 at 0.04 μg/ml.

All lanes:

Immunoprecipitation - Anti-SEC16A/SEC16 antibody (ab70722)

Predicted band size: 234 kDa

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Western blot - Anti-SEC16A/SEC16 antibody (AB70722)

All lanes:

Western blot - Anti-SEC16A/SEC16 antibody (ab70722) at 0.04 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 234 kDa

Observed band size: >250 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SEC16A. The exact immunogen used to generate this antibody is proprietary information.

O15027

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC16A also known as SEC16 is an important component in the secretory pathway specifically in vesicle trafficking from the endoplasmic reticulum (ER) to the Golgi apparatus. This protein plays a significant role in the assembly of coat protein complex II (COPII)-coated vesicles acting as a scaffold for other components. SEC16A has a molecular mass of approximately 240 kDa. It is broadly expressed in various tissues with notable expression in cells with high secretory activity.

Biological function summary

Functions of SEC16A are critical in maintaining ER exit sites (ERES) where the budding of COPII vesicles occurs. It localizes to these ERES facilitating the formation of transport vesicles. SEC16A does not work alone; it interacts with several other proteins to form a functional multi-protein complex associated with this vesicle trafficking process. These interactions ensure the efficient transport of proteins and lipids necessary for cellular homeostasis and secretion.

Pathways

SEC16A integrates into the COPII-mediated vesicle formation pathway pivotal for protein sorting and transportation. This protein collaborates closely with the SAR1 SEC23 SEC24 SEC13 and SEC31 proteins through the vesicle formation cycle. Besides its role in COPII assembly SEC16A also contributes to the regulation of ER-Golgi transport influencing the growth factor signaling pathways that depend on the accurate delivery of their receptor components to the cell surface.

Disruptions in the function of SEC16A links to conditions like Anderson-Fabry disease and congenital disorders of glycosylation. Abnormal vesicle trafficking due to SEC16A impairment can lead to an accumulation of metabolites or misfolded proteins that disrupt cellular function. SEC16A's relationship with proteins involved in lipid metabolism and glycosylation supports the role in these disorders. Understanding the behaviour of SEC16A in these contexts may contribute to targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Acts as a molecular scaffold that plays a key role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed : 17005010, PubMed : 17192411, PubMed : 17428803, PubMed : 21768384, PubMed : 22355596). Mediates the recruitment of MIA3/TANGO to ERES (PubMed : 28442536). Regulates both conventional (ER/Golgi-dependent) and GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane (PubMed : 28067262). Positively regulates the protein stability of E3 ubiquitin-protein ligases RNF152 and RNF183 and the ER localization of RNF183 (PubMed : 29300766). Acts as a RAB10 effector in the regulation of insulin-induced SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the cell membrane in adipocytes (By similarity).

See full target information SEC16A

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 81:334 PubMed39115595

2024

IER3IP1-mutations cause microcephaly by selective inhibition of ER-Golgi transport.

Applications

Unspecified application

Species

Unspecified reactive species

Mihaela Anitei,Francesca Bruno,Christina Valkova,Therese Dau,Emilio Cirri,Iván Mestres,Federico Calegari,Christoph Kaether

Nature cell biology 26:1077-1092 PubMed38926505

2024

A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Sun,Xuan Tao,Yaping Han,Xubo Lin,Rui Tian,Haodong Wang,Pei Chang,Qiming Sun,Liang Ge,Min Zhang

Journal of dental research 100:293-301 PubMed33034243

2020

and Autosomal Dominant Hypocalcified Amelogenesis Imperfecta.

Applications

Unspecified application

Species

Unspecified reactive species

S K Wang,H Zhang,C Y Hu,J F Liu,S Chadha,J W Kim,J P Simmer,J C C Hu

Frontiers in immunology 10:958 PubMed31114588

2019

NOD2 and TLR2 Signal via TBK1 and PI31 to Direct Cross-Presentation and CD8 T Cell Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Corridoni,Seiji Shiraishi,Thomas Chapman,Tessa Steevels,Daniele Muraro,Marie-Laëtitia Thézénas,Gennaro Prota,Ji-Li Chen,Uzi Gileadi,Nicola Ternette,Vincenzo Cerundolo,Alison Simmons

Nature 553:222-227 PubMed29323298

2018

A metabolic function of FGFR3-TACC3 gene fusions in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Véronique Frattini,Stefano M Pagnotta, Tala,Jerry J Fan,Marco V Russo,Sang Bae Lee,Luciano Garofano,Jing Zhang,Peiguo Shi,Genevieve Lewis,Heloise Sanson,Vanessa Frederick,Angelica M Castano,Luigi Cerulo,Delphine C M Rolland,Raghvendra Mall,Karima Mokhtari,Kojo S J Elenitoba-Johnson,Marc Sanson,Xi Huang,Michele Ceccarelli,Anna Lasorella,Antonio Iavarone

The Journal of biological chemistry 292:14050-14065 PubMed28710282

2017

Function of inhibitor of Bruton's tyrosine kinase isoform α (IBTKα) in nonalcoholic steatohepatitis links autophagy and the unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey A Willy,Sara K Young,Amber L Mosley,Samer Gawrieh,James L Stevens,Howard C Masuoka,Ronald C Wek

View all publications

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Product promise

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Anti-SEC16A/SEC16 antibody

Immunoprecipitation - Anti-SEC16A/SEC16 antibody (AB70722)

Western blot - Anti-SEC16A/SEC16 antibody (AB70722)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise