AB241585

Anti-SEC22B antibody

Name: Anti-SEC22B antibody (ab241585) | Abcam
Brand: Abcam Limited
SKU: AB241585
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SEC22B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SEC22B aa 100-150.

View Alternative Names

SEC22L1, SEC22B, Vesicle-trafficking protein SEC22b, ER-Golgi SNARE of 24 kDa, SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1, ERS-24, ERS24

2 Images

Supplier Data

Western blot - Anti-SEC22B antibody (AB241585)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-SEC22B antibody (ab241585) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 25 kDa

true

Exposure time: 30s

Supplier Data

Immunoprecipitation - Anti-SEC22B antibody (AB241585)

SEC22B was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241585 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241585 at 1 μg/ml.

Lane 1 : ab241585 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in RIPA buffer.

All lanes:

Immunoprecipitation - Anti-SEC22B antibody (ab241585)

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SEC22B aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

O75396

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to SEC22B immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC22B also known as SEC22 homolog B is a membrane protein involved in vesicular trafficking within cells. It plays an important role in the transport between the endoplasmic reticulum (ER) and the Golgi apparatus. SEC22B belongs to the SNARE (soluble NSF attachment protein receptor) family with a molecular mass of approximately 29 kDa. It is expressed in various tissues with prominent presence in the immune system and neuronal tissues. The protein participates in the assembly of vesicle transport machinery important for proper cellular function and compartmentalization.

Biological function summary

SEC22B contributes to the fusion of transport vesicles with target membranes. It acts as part of a SNARE complex facilitating vesicle docking and subsequent membrane fusion through protein-protein interaction. In immune cells SEC22B is essential for antigen presentation. It regulates the trafficking of peptide-MHC complexes to the cell surface essential for adaptive immune response. In neurons the protein ensures efficient neurotransmitter release by managing vesicle cycling and synaptic vesicle fusion.

Pathways

SEC22B integrates into the vesicular transport and secretory pathways. It operates in the ER-Golgi transport route critical in maintaining cellular homeostasis and protein processing. The function of SEC22B involves interaction with other SNARE proteins like VAMP7 and SNAP23 forming SNARE complexes that drive membrane fusion events. This protein also influences the Golgi-to-ER retrograde transport contributing to the protein sorting pathway that recycles specific proteins back to the ER.

SEC22B links closely to immune-related conditions and neurodegenerative diseases. Disruption in SEC22B function can impact antigen presentation and immune system efficiency potentially contributing to autoimmune disorders. In neurological contexts altered SEC22B activity may interfere with neurotransmitter release possibly playing a role in conditions such as Alzheimer's disease. Connections with proteins like VAMP7 underline the importance of SEC22B in maintaining cellular integrity across different systems.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.

See full target information SEC22B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 24:102700 PubMed34235408

2021

Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Shadrach,Wesley M Stansberry,Allison M Milen,Rachel E Ives,Elizabeth A Fogarty,Anthony Antonellis,Brian A Pierchala

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEC22B antibody

Western blot - Anti-SEC22B antibody (AB241585)

Immunoprecipitation - Anti-SEC22B antibody (AB241585)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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