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AB137583

Anti-SEC23A antibody

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(4 Publications)

Rabbit Polyclonal SEC23A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Protein transport protein Sec23A aa 100-450.

View Alternative Names

Protein transport protein Sec23A, hSec23A, SEC23-related protein A, SEC23A

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23A antibody (AB137583)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23A antibody (AB137583)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labeling SEC23A with ab137583 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-SEC23A antibody (AB137583)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SEC23A antibody (AB137583)

Immunofluorescence analysis of methanol-fixed HeLa cells labeling SEC23A with ab137583 at 1/200 dilution.

Western blot - Anti-SEC23A antibody (AB137583)
  • WB

Unknown

Western blot - Anti-SEC23A antibody (AB137583)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-SEC23A antibody (ab137583) at 1/10000 dilution

All lanes:

Molt-4 whole cell lysate at 30 µg

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Protein transport protein Sec23A aa 100-450. The exact immunogen used to generate this antibody is proprietary information.

Q15436

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC23A also known as Sar1p interaction protein is a component of the COPII-coated vesicle complex responsible for transport from the endoplasmic reticulum (ER) to the Golgi apparatus. With a mass of approximately 85 kDa SEC23A plays a mechanical role in vesicle formation by promoting coat complex assembly which is critical for cargo selection. This protein is expressed ubiquitously in various tissues especially in those with high secretory demands such as pancreas and liver.
Biological function summary

SEC23A functions as part of the COPII complex a group essential for vesicle budding from the ER. The interaction of SEC23A with other COPII components such as SEC24 allows it to select and package secretory proteins efficiently. This activity is not only important for protein trafficking but also for maintaining ER homeostasis and cellular function. Its certain role in the secretion pathway highlights the importance of proper protein sorting in cell and tissue health.

Pathways

SEC23A is an important player in the secretory pathway particularly involved in COPII-mediated vesicular transport. It interacts closely with SEC24 and Sar1 facilitating the transition of proteins through the early secretory pathway. Additionally SEC23A engages with lipid-related pathways contributing to lipid metabolism and transport ensuring the precise delivery of lipid-related proteins across organelles.

Mutations in SEC23A associate with cranio-lenticulo-sutural dysplasia (CLSD) a condition marked by skeletal and craniofacial abnormalities. This disorder is linked to the disruption in protein and collagen export due to impaired COPII function involving interactions between SEC23A and SEC24. Furthermore SEC23A is related to pancreatic disorders where dysfunction in COPII-mediated trafficking can lead to improper protein secretion and cellular stress affecting proteins such as insulin and digestive enzymes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex. Required for the translocation of insulin-induced glucose transporter SLC2A4/GLUT4 to the cell membrane (By similarity).
See full target information Protein transport protein Sec23A
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37373084

2023

New Cases and Mutations in Gene Causing Congenital Dyserythropoietic Anemia Type II.

Applications

Unspecified application

Species

Unspecified reactive species

Melina Mara Musri,Veronica Venturi,Xènia Ferrer-Cortès,Lídia Romero-Cortadellas,Gonzalo Hernández,Pilar Leoz,María Pilar Ricard Andrés,Marta Morado,María Del Carmen Fernández Valle,David Beneitez Pastor,Ana Ortuño Cabrero,Maite Moreno Gamiz,Leonor Senent Peris,Amanda Isabel Perez-Valencia,Santiago Pérez-Montero,Cristian Tornador,Mayka Sánchez

Journal of cancer research and clinical oncology 149:4771-4782 PubMed36229542

2022

Low TINAGL1 expression is a marker for poor prognosis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Akiko Kato,Naoto Kondo,Yumi Wanifuchi-Endo,Takashi Fujita,Tomoko Asano,Tomoka Hisada,Yasuaki Uemoto,Mitsuo Terada,Hiroyuki Kato,Masayuki Komura,Katsuhiro Okuda,Satoru Takahashi,Tatsuya Toyama

The Journal of cell biology 221: PubMed35829701

2022

Regulation of the COPII secretory machinery via focal adhesions and extracellular matrix signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Jung,Muzamil Majid Khan,Jonathan Landry,Aliaksandr Halavatyi,Pedro Machado,Miriam Reiss,Rainer Pepperkok

Oncotarget 7:30461-78 PubMed27036030

2016

MicroRNA-375/SEC23A as biomarkers of the in vitro efficacy of vandetanib.

Applications

WB, IHC

Species

Unspecified reactive species, Unspecified reactive species

Sandra Lassalle,Joséphine Zangari,Alexandra Popa,Marius Ilie,Véronique Hofman,Elodie Long,Martine Patey,Frédérique Tissier,Geneviève Belléannée,Hélène Trouette,Bogdan Catargi,Isabelle Peyrottes,Jean-Louis Sadoul,Olivier Bordone,Christelle Bonnetaud,Catherine Butori,Alexandre Bozec,Nicolas Guevara,José Santini,Imène Sarah Hénaoui,Géraldine Lemaire,Olivier Blanck,Philippe Vielh,Pascal Barbry,Bernard Mari,Patrick Brest,Paul Hofman
View all publications
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Product promise

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