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AB179811

Anti-SEC23A antibody [EPR13270(B)]

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(4 Publications)

Rabbit Recombinant Monoclonal SEC23A antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Protein transport protein Sec23A, hSec23A, SEC23-related protein A, SEC23A

3 Images
Flow Cytometry (Intracellular) - Anti-SEC23A antibody [EPR13270(B)] (AB179811)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SEC23A antibody [EPR13270(B)] (AB179811)

Intracellular flow cytometrical analysis of permeabilized HeLa cells labeling SEC23Awith ab179811 at 1/10 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-SEC23A antibody [EPR13270(B)] (AB179811)
  • WB

Lab

Western blot - Anti-SEC23A antibody [EPR13270(B)] (AB179811)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : SEC23A knockout HAP1 cell lysate (20 μg)
Lane 3 : HepG2 cell lysate (20 μg)
Lane 4 : HeLa cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab179811 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab179811 was shown to specifically react with SEC23A when SEC23A knockout samples were used. Wild-type and SEC23A knockout samples were subjected to SDS-PAGE. ab179811 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SEC23A antibody [EPR13270(B)] (ab179811)

Predicted band size: 86 kDa

Observed band size: 90 kDa

false

Western blot - Anti-SEC23A antibody [EPR13270(B)] (AB179811)
  • WB

Supplier Data

Western blot - Anti-SEC23A antibody [EPR13270(B)] (AB179811)

All lanes:

Western blot - Anti-SEC23A antibody [EPR13270(B)] (ab179811) at 1/10000 dilution

Lane 1:

HeLa cell lysate

Lane 2:

HepG2 cell lysate

Lane 3:

293T cell lysate

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 86 kDa

false

  • Carrier free

    Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13270(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC23A also known as Sar1p interaction protein is a component of the COPII-coated vesicle complex responsible for transport from the endoplasmic reticulum (ER) to the Golgi apparatus. With a mass of approximately 85 kDa SEC23A plays a mechanical role in vesicle formation by promoting coat complex assembly which is critical for cargo selection. This protein is expressed ubiquitously in various tissues especially in those with high secretory demands such as pancreas and liver.
Biological function summary

SEC23A functions as part of the COPII complex a group essential for vesicle budding from the ER. The interaction of SEC23A with other COPII components such as SEC24 allows it to select and package secretory proteins efficiently. This activity is not only important for protein trafficking but also for maintaining ER homeostasis and cellular function. Its certain role in the secretion pathway highlights the importance of proper protein sorting in cell and tissue health.

Pathways

SEC23A is an important player in the secretory pathway particularly involved in COPII-mediated vesicular transport. It interacts closely with SEC24 and Sar1 facilitating the transition of proteins through the early secretory pathway. Additionally SEC23A engages with lipid-related pathways contributing to lipid metabolism and transport ensuring the precise delivery of lipid-related proteins across organelles.

Mutations in SEC23A associate with cranio-lenticulo-sutural dysplasia (CLSD) a condition marked by skeletal and craniofacial abnormalities. This disorder is linked to the disruption in protein and collagen export due to impaired COPII function involving interactions between SEC23A and SEC24. Furthermore SEC23A is related to pancreatic disorders where dysfunction in COPII-mediated trafficking can lead to improper protein secretion and cellular stress affecting proteins such as insulin and digestive enzymes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex. Required for the translocation of insulin-induced glucose transporter SLC2A4/GLUT4 to the cell membrane (By similarity).
See full target information Protein transport protein Sec23A
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 221: PubMed35829701

2022

Regulation of the COPII secretory machinery via focal adhesions and extracellular matrix signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Jung,Muzamil Majid Khan,Jonathan Landry,Aliaksandr Halavatyi,Pedro Machado,Miriam Reiss,Rainer Pepperkok

JBMR plus 5:e10451 PubMed33778321

2021

Loss of TANGO1 Leads to Absence of Bone Mineralization.

Applications

Unspecified application

Species

Unspecified reactive species

Brecht Guillemyn,Sheela Nampoothiri,Delfien Syx,Fransiska Malfait,Sofie Symoens

Human molecular genetics 28:1801-1809 PubMed30657919

2019

A homozygous pathogenic missense variant broadens the phenotypic and mutational spectrum of CREB3L1-related osteogenesis imperfecta.

Applications

Unspecified application

Species

Unspecified reactive species

Brecht Guillemyn,Hülya Kayserili,Lynn Demuynck,Patrick Sips,Anne De Paepe,Delfien Syx,Paul J Coucke,Fransiska Malfait,Sofie Symoens

Genetics in medicine : official journal of the American College of Medical Genetics 20:411-419 PubMed28817112

2017

Monoallelic and biallelic CREB3L1 variant causes mild and severe osteogenesis imperfecta, respectively.

Applications

WB

Species

Unspecified reactive species

Rachel B Keller,Thao T Tran,Shawna M Pyott,Melanie G Pepin,Ravi Savarirayan,George McGillivray,Deborah A Nickerson,Michael J Bamshad,Peter H Byers
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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