AB151258

Anti-SEC23B antibody

Name: Anti-SEC23B antibody (ab151258) | Abcam
Brand: Abcam Limited
SKU: AB151258

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(5 Publications)

Rabbit Polyclonal SEC23B antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SEC23B aa 1-250.

View Alternative Names

Protein transport protein Sec23B, hSec23B, SEC23-related protein B, SEC23B

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB151258)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB151258)

Immunohistochemical analysis of paraffin embedded Human Ca922 oral cancer xenograft labeling SEC23B with ab151258 antibody at 1/500.

Unknown

Western blot - Anti-SEC23B antibody (AB151258)

7.5% SDS PAGE

All lanes:

Western blot - Anti-SEC23B antibody (ab151258) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 86 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SEC23B aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q15437

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Alternatively Tris-EDTA buffer ( pH8.0) may be used.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC23B also known as Protein Transport Protein Sec23B is a component of the coat protein complex II (COPII) which mediates transport from the endoplasmic reticulum to the Golgi apparatus. SEC23B weighs approximately 88 kDa and acts as a GTPase activating protein that regulates the COPII complex assembly. SEC23B broadly expresses in many tissues including high levels in the pancreas and liver suggesting its essential role in cellular transport processes.

Biological function summary

SEC23B functions in vesicular trafficking within cells. In the COPII complex it interacts with SEC24 proteins to form a structural framework critical for cargo selection and vesicle budding. SEC23B does not work alone and is part of a larger protein complex that includes SEC24 SAR1 and SEC13/31 which facilitates proper vesicle formation and trafficking. These interactions enable cells to accurately and efficiently transport necessary proteins and lipids through the secretory pathway.

Pathways

SEC23B is essential in the ER-to-Golgi transport pathway and plays a significant role in maintaining protein homeostasis. The secretory pathway requires SEC23B to cooperate with other proteins like SEC24C and SEC31A ensuring that cargo proteins are correctly packaged and delivered to their destination within the cell. This process is vital for the function of other pathways such as the unfolded protein response as it prevents the accumulation of misfolded proteins in the endoplasmic reticulum.

SEC23B mutations have links to Congenital Dyserythropoietic Anemia type II (CDA II) which manifests as ineffective erythropoiesis and improper glycosylation of red blood cell membrane proteins. SEC23B is also associated with pancreatic dysfunctions considering its high expression in the tissue although detailed mechanisms are less understood. In CDA II SEC23B's dysfunction affects its interaction with associated proteins like SEC24B disrupting normal vesicular trafficking and functional erythrocyte production.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.

See full target information SEC23B

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37373084

2023

New Cases and Mutations in Gene Causing Congenital Dyserythropoietic Anemia Type II.

Applications

Unspecified application

Species

Unspecified reactive species

Melina Mara Musri,Veronica Venturi,Xènia Ferrer-Cortès,Lídia Romero-Cortadellas,Gonzalo Hernández,Pilar Leoz,María Pilar Ricard Andrés,Marta Morado,María Del Carmen Fernández Valle,David Beneitez Pastor,Ana Ortuño Cabrero,Maite Moreno Gamiz,Leonor Senent Peris,Amanda Isabel Perez-Valencia,Santiago Pérez-Montero,Cristian Tornador,Mayka Sánchez

The Journal of clinical investigation 132: PubMed35143418

2022

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiong Liu,Minbiao Yan,Wanying Lei,Rencai Jiang,Wenxiu Dai,Jialin Chen,Chaomeng Wang,Li Li,Mei Wu,Ximing Nian,Daopeng Li,Di Sun,Xiaoqi Lv,Chaoying Wang,Changchuan Xie,Luming Yao,Caiming Wu,Jin Hu,Naian Xiao,Wei Mo,Zhanxiang Wang,Liang Zhang

Cell death & disease 12:304 PubMed33753724

2021

Non-canonical role of wild-type SEC23B in the cellular stress response pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lamis Yehia,Darren Liu,Shuai Fu,Pranav Iyer,Charis Eng

Human molecular genetics 27:3154-3164 PubMed29893852

2018

Non-canonical role of cancer-associated mutant SEC23B in the ribosome biogenesis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lamis Yehia,Supriya Jindal,Anton A Komar,Charis Eng

American journal of human genetics 97:661-76 PubMed26522472

2015

Germline Heterozygous Variants in SEC23B Are Associated with Cowden Syndrome and Enriched in Apparently Sporadic Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lamis Yehia,Farshad Niazi,Ying Ni,Joanne Ngeow,Madhav Sankunny,Zhigang Liu,Wei Wei,Jessica L Mester,Ruth A Keri,Bin Zhang,Charis Eng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SEC23B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB151258)

Western blot - Anti-SEC23B antibody (AB151258)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Complementary Products

Primary Antibodies

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Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise