AB251694

Anti-SEC23B antibody

Name: Anti-SEC23B antibody (ab251694) | Abcam
Brand: Abcam Limited
SKU: AB251694
Price: 655 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal SEC23B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEC23B aa 150-300.

View Alternative Names

Protein transport protein Sec23B, hSec23B, SEC23-related protein B, SEC23B

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB251694)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB251694)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue shows absence of immunoreactivity for SEC23B when incubated with ab251694 at a 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SEC23B antibody (AB251694)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SEC23B antibody (AB251694)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for SEC23B (green) using ab251694 at 4 µg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB251694)

Formalin-fixed, paraffin-embedded human prostate tissue stained for SEC23B with ab251694 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB251694)

Formalin-fixed, paraffin-embedded human testis tissue stained for SEC23B with ab251694 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB251694)

Formalin-fixed, paraffin-embedded human Fallopian tube tissue stained for SEC23B with ab251694 at a 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SEC23B aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q15437

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC23B also known as Protein Transport Protein Sec23B is a component of the coat protein complex II (COPII) which mediates transport from the endoplasmic reticulum to the Golgi apparatus. SEC23B weighs approximately 88 kDa and acts as a GTPase activating protein that regulates the COPII complex assembly. SEC23B broadly expresses in many tissues including high levels in the pancreas and liver suggesting its essential role in cellular transport processes.

Biological function summary

SEC23B functions in vesicular trafficking within cells. In the COPII complex it interacts with SEC24 proteins to form a structural framework critical for cargo selection and vesicle budding. SEC23B does not work alone and is part of a larger protein complex that includes SEC24 SAR1 and SEC13/31 which facilitates proper vesicle formation and trafficking. These interactions enable cells to accurately and efficiently transport necessary proteins and lipids through the secretory pathway.

Pathways

SEC23B is essential in the ER-to-Golgi transport pathway and plays a significant role in maintaining protein homeostasis. The secretory pathway requires SEC23B to cooperate with other proteins like SEC24C and SEC31A ensuring that cargo proteins are correctly packaged and delivered to their destination within the cell. This process is vital for the function of other pathways such as the unfolded protein response as it prevents the accumulation of misfolded proteins in the endoplasmic reticulum.

SEC23B mutations have links to Congenital Dyserythropoietic Anemia type II (CDA II) which manifests as ineffective erythropoiesis and improper glycosylation of red blood cell membrane proteins. SEC23B is also associated with pancreatic dysfunctions considering its high expression in the tissue although detailed mechanisms are less understood. In CDA II SEC23B's dysfunction affects its interaction with associated proteins like SEC24B disrupting normal vesicular trafficking and functional erythrocyte production.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.

See full target information SEC23B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 9:478 PubMed33850875

2021

Low expression of BTN3A3 indicates poor prognosis and promotes cell proliferation, migration and invasion in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Cheng,Tianyu Ma,Ling Yi,Chongyu Su,Xiaojue Wang,Tao Wen,Bing Wang,Yuxuan Wang,Hongtao Zhang,Zhidong Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEC23B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB251694)

Immunocytochemistry/ Immunofluorescence - Anti-SEC23B antibody (AB251694)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB251694)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB251694)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody (AB251694)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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