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Rabbit Recombinant Monoclonal SEC23B antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750), expandable thumbnail
  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750), expandable thumbnail
  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.

Additional Targets

Protein transport protein Sec23A

Alternative names

Protein transport protein Sec23B, hSec23B, SEC23-related protein B, SEC23B

Recommended products

Rabbit Recombinant Monoclonal SEC23B antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22723-102
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SEC23B and SEC23A are proteins involved in the formation of transport vesicles from the endoplasmic reticulum (ER) to the Golgi apparatus. These proteins are part of the COPII coat protein complex. SEC23B has a mass of approximately 92 kDa while SEC23A is about 88 kDa. Both proteins are widely expressed with significant levels in the liver pancreas and other tissues. SEC23B and SEC23A share high sequence homology reflecting similar roles within cells.

Biological function summary

SEC23B and SEC23A are key components of the COPII complex working together with SEC24 SEC13 and SEC31 to facilitate the budding of vesicles from the ER. This complex regulates cargo selection and vesicle budding ensuring correct protein transport. Both proteins interact closely with SAR1 a small GTPase that initiates COPII coat assembly. The functionality of SEC23B and SEC23A is significant in maintaining cellular homeostasis by ensuring the efficient transport of proteins.

Pathways

SEC23B and SEC23A are integral in the secretory pathway a fundamental process in cell biology. The secretory pathway involves the transport of newly synthesized proteins from the ER to the Golgi and ultimately to their final destinations. Within this pathway SEC23B and SEC23A work alongside SEC24 to select cargo proteins that need transport. Their activities are connected to the vesicular transport pathway emphasizing the interplay with SAR1 and other COPII components.

Associated diseases and disorders

SEC23B mutations are linked to Congenital Dyserythropoietic Anemia type II (CDAII) which affects red blood cell production and function. This disorder highlights SEC23B’s role in erythroid cell development. Moreover SEC23A mutations are associated with cranio-lenticulo-sutural dysplasia a rare disease impacting craniofacial and skeletal development. The involvement of SEC23B and SEC23A in these disorders indicates their essential roles in specialized cell processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive cytoplasmic staining on rat kidney (PMID: 22745161).

    The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750), expandable thumbnail

    Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22745161).

    Exposure time: Lanes 1-4: 26 seconds;Lanes 5-8: 10 seconds.

    All lanes: Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

    Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Lane 5: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 6: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 7: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 8: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Lane 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 20000 mg/mL

    Observed band size: 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive cytoplasmic staining on mouse liver (PMID: 22745161).

    The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750), expandable thumbnail

    Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22745161).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

    All lanes: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Observed band size: 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive cytoplasmic staining on human cerebrum (PMID: 22745161) is observed.

    The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750), expandable thumbnail

    Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Both the recombinant proteins were made in house.

    SEC23B recombinant protein was expressed from a mammalian - HEK-293 expression system. The LIF tag is about 40 kDa.

    SEC23A recombinant protein was from E.coil extracts.

    Exposure time: 3 seconds.

    All lanes: Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

    Lane 1: Purified LIF/His-tagged SEC23B recombinant protein (aa127-392), 10 ng

    Lane 2: His-tagged SEC23A recombinant protein (aa127-392)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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