AB270750

Anti-SEC23B + SEC23A antibody [EPR22723-102]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal SEC23B antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples.

View Alternative Names

Protein transport protein Sec23B, hSec23B, SEC23-related protein B, SEC23B, Protein transport protein Sec23A, hSec23A, SEC23-related protein A, SEC23A

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on human cerebrum (PMID : 22745161) is observed.

The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on rat kidney (PMID : 22745161).

The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on mouse liver (PMID : 22745161).

The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 22745161).

Exposure time : Lanes 1-4 : 26 seconds;Lanes 5-8 : 10 seconds.

All lanes:

Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

Lane 1:

MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 6:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 8:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

Lanes 1 - 7:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lane 8:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 20000 mg/mL

Observed band size: 86 kDa

false

Lab

Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 22745161).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

All lanes:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 86 kDa

false

Lab

Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Both the recombinant proteins were made in house.

SEC23B recombinant protein was expressed from a mammalian - HEK-293 expression system. The LIF tag is about 40 kDa.

SEC23A recombinant protein was from E.coil extracts.

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

Lane 1:

Purified LIF/His-tagged SEC23B recombinant protein (aa127-392), 10 ng

Lane 2:

His-tagged SEC23A recombinant protein (aa127-392)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Carrier free

Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22723-102

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC23B and SEC23A are proteins involved in the formation of transport vesicles from the endoplasmic reticulum (ER) to the Golgi apparatus. These proteins are part of the COPII coat protein complex. SEC23B has a mass of approximately 92 kDa while SEC23A is about 88 kDa. Both proteins are widely expressed with significant levels in the liver pancreas and other tissues. SEC23B and SEC23A share high sequence homology reflecting similar roles within cells.

Biological function summary

SEC23B and SEC23A are key components of the COPII complex working together with SEC24 SEC13 and SEC31 to facilitate the budding of vesicles from the ER. This complex regulates cargo selection and vesicle budding ensuring correct protein transport. Both proteins interact closely with SAR1 a small GTPase that initiates COPII coat assembly. The functionality of SEC23B and SEC23A is significant in maintaining cellular homeostasis by ensuring the efficient transport of proteins.

Pathways

SEC23B and SEC23A are integral in the secretory pathway a fundamental process in cell biology. The secretory pathway involves the transport of newly synthesized proteins from the ER to the Golgi and ultimately to their final destinations. Within this pathway SEC23B and SEC23A work alongside SEC24 to select cargo proteins that need transport. Their activities are connected to the vesicular transport pathway emphasizing the interplay with SAR1 and other COPII components.

SEC23B mutations are linked to Congenital Dyserythropoietic Anemia type II (CDAII) which affects red blood cell production and function. This disorder highlights SEC23B’s role in erythroid cell development. Moreover SEC23A mutations are associated with cranio-lenticulo-sutural dysplasia a rare disease impacting craniofacial and skeletal development. The involvement of SEC23B and SEC23A in these disorders indicates their essential roles in specialized cell processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.

See full target information SEC23B

Additional targets

Protein transport protein Sec23A

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEC23B + SEC23A antibody [EPR22723-102]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (AB270750)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise