Rabbit Polyclonal SEC23IP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEC23IP aa 850-950.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SEC23IP aa 850-950. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6Y8
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a role in the organization of endoplasmic reticulum exit sites. Specifically binds to phosphatidylinositol 3-phosphate (PI(3)P), phosphatidylinositol 4-phosphate (PI(4)P) and phosphatidylinositol 5-phosphate (PI(5)P).
Alternative names
MSTP053, SEC23IP, SEC23-interacting protein, p125
Recommended products
Rabbit Polyclonal SEC23IP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEC23IP aa 850-950.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SEC23IP aa 850-950. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6Y8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEC23IP also known as SEC23-interacting protein or p125 serves an important role in the assembly and functioning of the COPII complex. It weighs approximately 115 kDa and participates in transporting proteins from the endoplasmic reticulum to the Golgi apparatus. SEC23IP binds to SEC23 a component of the COPII coat and modulates the assembly process for vesicle formation. Expression of SEC23IP occurs in many tissues with significant levels observed in the liver and pancreas.
- Biological function summary
SEC23IP assists in mediating the selection and transport of cargo molecules by stabilizing the SEC23/24 subcomplex within the larger COPII complex. SEC23IP therefore helps facilitate the budding of transport vesicles from the endoplasmic reticulum. By acting within this protein complex SEC23IP enhances the fidelity and specificity of cargo selection and compartmentalization essential for functional protein sorting.
- Pathways
SEC23IP acts as an indispensable component of the secretory pathway which ensures the proper trafficking of proteins through the cell. It interacts largely within the context of the COPII vesicle-mediated transport pathway and is influenced by proteins such as SAR1 and SEC24. These transport routes are necessary for maintaining cellular homeostasis and enabling cell surface membrane depiction.
- Associated diseases and disorders
Alterations in SEC23IP expression or function have connections to conditions involving improper protein trafficking. One known association is with craniofacial abnormalities linked to disruptions in cellular transport processes. Mutations in related COPII proteins like SEC23 also highlight the consequences on skeletal development and metabolism which can manifest in conditions such as cranio-lenticulo-sutural dysplasia (CLSD). Understanding the exact role of SEC23IP contributes to insights into these developmental disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-SEC23IP antibody (ab224546)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SEC23IP (green) using ab224546 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23IP antibody (ab224546)
Paraffin-embedded human stomach tissue stained for SEC23IP using ab224546 at 1/20 dilution in immunohistochemical analysis.
Western blot - Anti-SEC23IP antibody (ab224546)
All lanes: Western blot - Anti-SEC23IP antibody (ab224546) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2: NBT-II cell lysate
Predicted band size: 111 kDa
Downloads
Product protocols
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