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AB262869

Anti-SEC24A antibody

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(2 Publications)

Rabbit Polyclonal SEC24A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Protein transport protein Sec24A aa 100-250.

View Alternative Names

Protein transport protein Sec24A, SEC24-related protein A, SEC24A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC24A antibody (AB262869)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC24A antibody (AB262869)

Paraffin-embedded human stomach tissue stained for SEC24A using ab262869 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-SEC24A antibody (AB262869)
  • WB

Unknown

Western blot - Anti-SEC24A antibody (AB262869)

All lanes:

Western blot - Anti-SEC24A antibody (ab262869) at 0.4 µg/mL

Lane 1:

Caco-2 cell lysate

Lane 2:

PC-3 cell lysate

Predicted band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Protein transport protein Sec24A aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

O95486

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC24A also known as SEC24-related gene family member A is a protein that participates in the process of vesicle formation. It has a molecular mass of approximately 121 kDa. SEC24A is expressed in various tissues including the liver brain and pancreas. It plays an important role in the secretory pathway by helping select and package cargo proteins into vesicles that bud from the endoplasmic reticulum (ER). This protein works alongside other components of the COPII complex to ensure specific transport of proteins to their correct cellular destinations.
Biological function summary

SEC24A functions as part of the COPII vesicle coat complex in eukaryotic cells. It helps in selecting and recognizing cargo proteins for transport from the ER to the Golgi apparatus. By binding directly to cargo proteins or indirectly through adaptor proteins SEC24A aids in their incorporation into vesicles. The COPII complex which also includes SEC23 SEC13 and SEC31 is important for effective protein trafficking and maintenance of cellular homeostasis.

Pathways

SEC24A participates significantly in the secretory pathway and ER-to-Golgi transport. It interacts with other pathway proteins such as SAR1 and SEC23 which are essential for COPII vesicle budding and fusion. SEC24A's function in these pathways is critical for cellular processes such as hormone release extracellular matrix construction and lipid metabolism. Its activity ensures proper protein distribution directly affecting cell growth and survival.

SEC24A has connections to cardiovascular diseases and metabolic disorders. Mutations or dysregulation of SEC24A may result in disturbed lipid metabolism contributing to conditions like hyperlipidemia. Additionally the protein's involvement in vesicle trafficking links it to cardiac diseases where improper protein transport can lead to cellular stress and dysfunction. SEC24A's interactive role with proteins like SAR1 can lead to disruptions when they are not functioning properly further emphasizing its impact on disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (PubMed : 17499046, PubMed : 18843296, PubMed : 20427317). Plays a central role in cargo selection within the COPII complex and together with SEC24B may have a different specificity compared to SEC24C and SEC24D. May package preferentially cargos with cytoplasmic DxE or LxxLE motifs and may also recognize conformational epitopes (PubMed : 17499046, PubMed : 18843296).
See full target information Protein transport protein Sec24A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2535 PubMed40087276

2025

ER O-glycosylation in synovial fibroblasts drives cartilage degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Le Son Tran,Joanne Chia,Xavier Le Guezennec,Keit Min Tham,Anh Tuan Nguyen,Virginie Sandrin,Way Cherng Chen,Tan Tong Leng,Sreedharan Sechachalam,Khai Pang Leong,Frederic A Bard

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405127 PubMed39361436

2024

SEC31a-ATG9a Interaction Mediates the Recruitment of COPII Vesicles for Autophagosome Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaming Nie,Shaoyang Ma,Linyue Wu,Ye Li,Jiao Cao,Meng Li,Peter Mei,Paul R Cooper,Ang Li,Dandan Pei
View all publications
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