Rabbit Polyclonal SEC24A antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein transport protein Sec24A aa 100-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Protein transport protein Sec24A aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link O95486
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.4 µg/mL | Notes - |
Target data
Function
Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (PubMed:17499046, PubMed:18843296, PubMed:20427317). Plays a central role in cargo selection within the COPII complex and together with SEC24B may have a different specificity compared to SEC24C and SEC24D. May package preferentially cargos with cytoplasmic DxE or LxxLE motifs and may also recognize conformational epitopes (PubMed:17499046, PubMed:18843296).
Alternative names
Protein transport protein Sec24A, SEC24-related protein A, SEC24A
Recommended products
Rabbit Polyclonal SEC24A antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein transport protein Sec24A aa 100-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Protein transport protein Sec24A aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link O95486
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEC24A also known as SEC24-related gene family member A is a protein that participates in the process of vesicle formation. It has a molecular mass of approximately 121 kDa. SEC24A is expressed in various tissues including the liver brain and pancreas. It plays an important role in the secretory pathway by helping select and package cargo proteins into vesicles that bud from the endoplasmic reticulum (ER). This protein works alongside other components of the COPII complex to ensure specific transport of proteins to their correct cellular destinations.
- Biological function summary
SEC24A functions as part of the COPII vesicle coat complex in eukaryotic cells. It helps in selecting and recognizing cargo proteins for transport from the ER to the Golgi apparatus. By binding directly to cargo proteins or indirectly through adaptor proteins SEC24A aids in their incorporation into vesicles. The COPII complex which also includes SEC23 SEC13 and SEC31 is important for effective protein trafficking and maintenance of cellular homeostasis.
- Pathways
SEC24A participates significantly in the secretory pathway and ER-to-Golgi transport. It interacts with other pathway proteins such as SAR1 and SEC23 which are essential for COPII vesicle budding and fusion. SEC24A's function in these pathways is critical for cellular processes such as hormone release extracellular matrix construction and lipid metabolism. Its activity ensures proper protein distribution directly affecting cell growth and survival.
- Associated diseases and disorders
SEC24A has connections to cardiovascular diseases and metabolic disorders. Mutations or dysregulation of SEC24A may result in disturbed lipid metabolism contributing to conditions like hyperlipidemia. Additionally the protein's involvement in vesicle trafficking links it to cardiac diseases where improper protein transport can lead to cellular stress and dysfunction. SEC24A's interactive role with proteins like SAR1 can lead to disruptions when they are not functioning properly further emphasizing its impact on disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SEC24A antibody (ab262869)
All lanes: Western blot - Anti-SEC24A antibody (ab262869) at 0.4 µg/mL
Lane 1: Caco-2 cell lysate
Lane 2: PC-3 cell lysate
Predicted band size: 120 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC24A antibody (ab262869)
Paraffin-embedded human stomach tissue stained for SEC24A using ab262869 at 1/50 dilution in immunohistochemical analysis.
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