Rabbit Polyclonal SEC24B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein transport protein Sec24B aa 100-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Protein transport protein Sec24B aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link O95487
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (PubMed:17499046, PubMed:18843296, PubMed:20427317). Plays a central role in cargo selection within the COPII complex and together with SEC24A may have a different specificity compared to SEC24C and SEC24D. May package preferentially cargos with cytoplasmic DxE or LxxLE motifs and may also recognize conformational epitopes (PubMed:17499046, PubMed:18843296).
Alternative names
Protein transport protein Sec24B, SEC24-related protein B, SEC24B
Recommended products
Rabbit Polyclonal SEC24B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein transport protein Sec24B aa 100-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Protein transport protein Sec24B aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link O95487
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEC24B also known as Sec24-related protein B is a component of the COPII (coat protein complex II) vesicle coat. It has a molecular mass of approximately 139 kDa. SEC24B serves as an essential element in the machinery that assists protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus. The protein facilitates the packaging of cargo proteins into vesicles which are small membrane-bound sacs. SEC24B is widely expressed in various tissues including the liver brain heart and kidneys.
- Biological function summary
The role of SEC24B involves facilitating selective cargo sorting in the ER-to-Golgi transport process. SEC24B is part of a larger protein complex necessary for forming COPII vesicles which are critical for exporting secretory proteins. This protein interacts with transport proteins and various cargo receptors to identify and selectively package proteins destined for the Golgi apparatus enabling the efficient sorting and delivery of proteins necessary for cellular function.
- Pathways
SEC24B is integral to the secretory pathway that maintains cellular protein traffic from the ER to the Golgi. It cooperates with proteins like SAR1 and SEC23 to form the prebudding complex on the ER membrane. SEC24B and these related proteins work together to mediate vesicle budding and cargo selection. Additionally SEC24B plays a part in the vesicular transport pathway which ensures proper protein localization and function within the cell.
- Associated diseases and disorders
SEC24B has associations with disorders like congenital heart defects and neural tube defects. Alterations in SEC24B’s function can impact the development and proper formation of cardiac and neural tissues. SEC24B interacts with other proteins like SEC23 which have links to the presentation of these developmental disorders highlighting its importance in cellular export pathways during development. Understanding SEC24B's roles might provide insights into potential therapeutic approaches for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-SEC24B antibody (ab122445)
Immunocytochemistry/immunofluorescence analysis of human cell line A-431 labelling SEC24B with ab122445 at 2 μg/mL shows localization to nucleoplasm & vesicles.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC24B antibody (ab122445)
ab122445, at 1/200 dilution, staining SEC24B in paraffin-embedded Human kidney tissue by Immunohistochemistry.
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