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AB241336

Anti-Sec24C antibody

4

(2 Reviews)

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(2 Publications)

Rabbit Polyclonal Sec24C antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SEC24C aa 1-50.

View Alternative Names

KIAA0079, SEC24C, Protein transport protein Sec24C, SEC24-related protein C

2 Images
Immunoprecipitation - Anti-Sec24C antibody (AB241336)
  • IP

Supplier Data

Immunoprecipitation - Anti-Sec24C antibody (AB241336)

Sec24C was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241336 used for IP at 6 μg per reaction. For WB 0.4 μg/ml.

Lane 1 : ab241336 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Chemiluminescence detection : 1 seconds.

All lanes:

Immunoprecipitation - Anti-Sec24C antibody (ab241336)

Predicted band size: 118 kDa

false

Western blot - Anti-Sec24C antibody (AB241336)
  • WB

Supplier Data

Western blot - Anti-Sec24C antibody (AB241336)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Sec24C antibody (ab241336) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 118 kDa

true

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SEC24C aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P53992

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Sec24C also known as SEC24 family member C functions in vesicular trafficking. It belongs to the COPII coat complex which plays a role in transporting proteins from the endoplasmic reticulum to the Golgi apparatus. Sec24C is part of a family with a molecular mass of approximately 125 kDa and it is ubiquitously expressed in various tissues suggesting an essential role in cellular transport mechanisms.
Biological function summary

Sec24C is involved in the formation of COPII-coated vesicles which are essential for the anterograde transport of proteins. It serves as a cargo adaptor within the COPII complex consisting also of proteins such as Sec23 Sec13 and Sec31. The interaction of Sec24C with these proteins ensures the proper selection and packaging of cargo during vesicle budding therefore maintaining effective intracellular transport.

Pathways

Sec24C plays a role in the secretory pathway and is involved in the ER-to-Golgi transport. It functions in collaboration with other COPII components facilitating the packaging and movement of secretory proteins. Key proteins associated with this pathways include Sar1 and Sec23 which coordinate with Sec24C to regulate vesicle formation and cargo selection efficiently.

Disruptions in Sec24C function have been linked to neurological conditions such as Alzheimer's disease and certain forms of neurodegeneration. Altered Sec24C activity affects vesicle trafficking and protein processing interacting with proteins like APP (Amyloid precursor protein) which may lead to pathology. Understanding the role of Sec24C and its interactions could offer insights into therapeutic strategies for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (PubMed : 10214955, PubMed : 17499046, PubMed : 18843296, PubMed : 20427317). Plays a central role in cargo selection within the COPII complex and together with SEC24D may have a different specificity compared to SEC24A and SEC24B (PubMed : 17499046, PubMed : 18843296, PubMed : 20427317). May more specifically package GPI-anchored proteins through the cargo receptor TMED10 (PubMed : 20427317). May also be specific for IxM motif-containing cargos like the SNAREs GOSR2 and STX5 (PubMed : 18843296).
See full target information SEC24C
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:6977 PubMed36379959

2022

Interruption of post-Golgi STING trafficking activates tonic interferon signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xintao Tu,Ting-Ting Chu,Devon Jeltema,Kennady Abbott,Kun Yang,Cong Xing,Jie Han,Nicole Dobbs,Nan Yan

Nature communications 13:646 PubMed35115501

2022

Schwann cell endosome CGRP signals elicit periorbital mechanical allodynia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco De Logu,Romina Nassini,Alan Hegron,Lorenzo Landini,Dane D Jensen,Rocco Latorre,Julia Ding,Matilde Marini,Daniel Souza Monteiro de Araujo,Paulina Ramírez-Garcia,Michael Whittaker,Jeffri Retamal,Mustafa Titiz,Alessandro Innocenti,Thomas P Davis,Nicholas Veldhuis,Brian L Schmidt,Nigel W Bunnett,Pierangelo Geppetti
View all publications
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