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Rabbit Recombinant Monoclonal SEC24D antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-SEC24D antibody [EPR16089] - BSA and Azide free (AB251088), expandable thumbnail
  • Western blot - Anti-SEC24D antibody [EPR16089] - BSA and Azide free (AB251088), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (PubMed:17499046, PubMed:18843296, PubMed:20427317). Plays a central role in cargo selection within the COPII complex and together with SEC24C may have a different specificity compared to SEC24A and SEC24B (PubMed:17499046, PubMed:18843296, PubMed:20427317). May more specifically package GPI-anchored proteins through the cargo receptor TMED10 (PubMed:20427317). May also be specific for IxM motif-containing cargos like the SNAREs GOSR2 and STX5 (PubMed:18843296).

Alternative names

KIAA0755, SEC24D, Protein transport protein Sec24D, SEC24-related protein D

Recommended products

Rabbit Recombinant Monoclonal SEC24D antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16089
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251088 is the carrier-free version of Anti-SEC24D antibody [EPR16089] - N-terminal ab191566.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SEC24D also known as SEC24-related protein D plays an important role in the transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus by mediating cargo selection for vesicle budding. This protein is part of the COPII coat complex. SEC24D has a molecular mass of approximately 130 kDa. It is widely expressed in most tissues with higher expression levels observed in the liver and testes. Its function in vesicular trafficking makes it essential for maintaining cellular protein transport.

Biological function summary

The protein is involved in the packaging of cargo into COPII vesicles. It operates within the COPII complex which includes proteins like SEC23 SEC13 SEC31 and SAR1. SEC24D functions by recognizing and binding specific cargo sorting signals ensuring proteins reach their correct destination. This process is fundamental in maintaining proper cellular organization and function by facilitating efficient secretory pathways.

Pathways

The SEC24D protein is important in the secretory pathway one of two key pathways for protein transport. It collaborates with the COPII machinery linking endoplasmic reticulum export to various cellular functions. SEC24D works closely with SEC23 in forming budding vesicles which eventually fuse with the Golgi apparatus. The COPII pathway is tightly connected with the ER stress response pathway ensuring cellular adaptation to changes in protein-folding demands.

Associated diseases and disorders

SEC24D mutations have been linked to congenital disorders of glycosylation and skeletal abnormalities such as osteogenesis imperfecta. SEC24D interacts with other components of the COPII complex like SEC23 which also play a role in these conditions. Understanding the function of SEC24D can provide insights into therapeutic strategies aimed at correcting dysregulated protein trafficking pathways associated with such disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-SEC24D antibody [EPR16089] - BSA and Azide free (ab251088), expandable thumbnail

    Western blot - Anti-SEC24D antibody [EPR16089] - BSA and Azide free (ab251088)

    This data was developed using Anti-SEC24D antibody [EPR16089] - N-terminal ab191566, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (Anti-SEC24D antibody [EPR16089] - N-terminal ab191566) at 1/5000 dilution

    Lane 1: SH-SY5Y cell lysate at 20 µg

    Lane 2: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 113 kDa

    Observed band size: 113 kDa

  • Western blot - Anti-SEC24D antibody [EPR16089] - BSA and Azide free (ab251088), expandable thumbnail

    Western blot - Anti-SEC24D antibody [EPR16089] - BSA and Azide free (ab251088)

    This data was developed using Anti-SEC24D antibody [EPR16089] - N-terminal ab191566, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (Anti-SEC24D antibody [EPR16089] - N-terminal ab191566) at 1/1000 dilution

    Lane 1: Raw264.7 cell lysate at 10 µg

    Lane 2: PC-12 cell lysate at 10 µg

    Lane 3: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 113 kDa

    Observed band size: 113 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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