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AB191566

Anti-SEC24D antibody [EPR16089] - N-terminal

3

(2 Reviews)

|

(7 Publications )

Rabbit Recombinant Monoclonal SEC24D antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

KIAA0755, SEC24D, Protein transport protein Sec24D, SEC24-related protein D

2 Images
Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (AB191566)
  • WB

Supplier Data

Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (AB191566)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566) at 1/5000 dilution

Lane 1:

SH-SY5Y cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 113 kDa

Observed band size: 113 kDa

false

Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (AB191566)
  • WB

Supplier Data

Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (AB191566)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566) at 1/1000 dilution

Lane 1:

Raw264.7 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 113 kDa

Observed band size: 113 kDa

false

  • Carrier free

    Anti-SEC24D antibody [EPR16089] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16089

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC24D also known as SEC24-related protein D plays an important role in the transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus by mediating cargo selection for vesicle budding. This protein is part of the COPII coat complex. SEC24D has a molecular mass of approximately 130 kDa. It is widely expressed in most tissues with higher expression levels observed in the liver and testes. Its function in vesicular trafficking makes it essential for maintaining cellular protein transport.
Biological function summary

The protein is involved in the packaging of cargo into COPII vesicles. It operates within the COPII complex which includes proteins like SEC23 SEC13 SEC31 and SAR1. SEC24D functions by recognizing and binding specific cargo sorting signals ensuring proteins reach their correct destination. This process is fundamental in maintaining proper cellular organization and function by facilitating efficient secretory pathways.

Pathways

The SEC24D protein is important in the secretory pathway one of two key pathways for protein transport. It collaborates with the COPII machinery linking endoplasmic reticulum export to various cellular functions. SEC24D works closely with SEC23 in forming budding vesicles which eventually fuse with the Golgi apparatus. The COPII pathway is tightly connected with the ER stress response pathway ensuring cellular adaptation to changes in protein-folding demands.

SEC24D mutations have been linked to congenital disorders of glycosylation and skeletal abnormalities such as osteogenesis imperfecta. SEC24D interacts with other components of the COPII complex like SEC23 which also play a role in these conditions. Understanding the function of SEC24D can provide insights into therapeutic strategies aimed at correcting dysregulated protein trafficking pathways associated with such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (PubMed : 17499046, PubMed : 18843296, PubMed : 20427317). Plays a central role in cargo selection within the COPII complex and together with SEC24C may have a different specificity compared to SEC24A and SEC24B (PubMed : 17499046, PubMed : 18843296, PubMed : 20427317). May more specifically package GPI-anchored proteins through the cargo receptor TMED10 (PubMed : 20427317). May also be specific for IxM motif-containing cargos like the SNAREs GOSR2 and STX5 (PubMed : 18843296).
See full target information SEC24D
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37629027

2023

Aerobic Exercise Facilitates the Nuclear Translocation of SREBP2 by Activating AKT/SEC24D to Contribute Cholesterol Homeostasis for Improving Cognition in APP/PS1 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zelin Hu,Yangqi Yuan,Zhen Tong,Meiqing Liao,Shunling Yuan,Weijia Wu,Yingzhe Tang,Yirong Wang,Changfa Tang,Wenfeng Liu

Life science alliance 5: PubMed35396334

2022

The hexosamine pathway and coat complex II promote malignant adaptation to nutrient scarcity.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Dragic,Audrey Barthelaix,Cédric Duret,Simon Le Goupil,Hadrien Laprade,Sophie Martin,Sabine Brugière,Yohann Couté,Christelle Machon,Jerome Guitton,Justine Rudewicz,Paul Hofman,Serge Lebecque,Cedric Chaveroux,Carole Ferraro-Peyret,Toufic Renno,Serge N Manié

JBMR plus 5:e10451 PubMed33778321

2021

Loss of TANGO1 Leads to Absence of Bone Mineralization.

Applications

Unspecified application

Species

Unspecified reactive species

Brecht Guillemyn,Sheela Nampoothiri,Delfien Syx,Fransiska Malfait,Sofie Symoens

PloS one 15:e0233502 PubMed32433667

2020

ERp29 as a regulator of Insulin biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Viviano,Margaret Brecker,Christine Ferrara-Cook,Laurence Suaud,Ronald C Rubenstein

Human molecular genetics 28:1801-1809 PubMed30657919

2019

A homozygous pathogenic missense variant broadens the phenotypic and mutational spectrum of CREB3L1-related osteogenesis imperfecta.

Applications

Unspecified application

Species

Unspecified reactive species

Brecht Guillemyn,Hülya Kayserili,Lynn Demuynck,Patrick Sips,Anne De Paepe,Delfien Syx,Paul J Coucke,Fransiska Malfait,Sofie Symoens

The Journal of cell biology 216:4153-4164 PubMed29133483

2017

deletion in pancreatic β cells impairs proinsulin trafficking and insulin biogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Junwan Fan,Yaqing Wang,Liang Liu,Hongsheng Zhang,Feng Zhang,Lei Shi,Mei Yu,Fei Gao,Zhiheng Xu

Genetics in medicine : official journal of the American College of Medical Genetics 20:411-419 PubMed28817112

2017

Monoallelic and biallelic CREB3L1 variant causes mild and severe osteogenesis imperfecta, respectively.

Applications

WB

Species

Unspecified reactive species

Rachel B Keller,Thao T Tran,Shawna M Pyott,Melanie G Pepin,Ravi Savarirayan,George McGillivray,Deborah A Nickerson,Michael J Bamshad,Peter H Byers
View all publications
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Product promise

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