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AB253009

Anti-Sec31A antibody

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(1 Publication)

Rabbit Polyclonal Sec31A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEC31A aa 600 to C-terminus.

View Alternative Names

KIAA0905, SEC31L1, HSPC275, HSPC334, SEC31A, Protein transport protein Sec31A, ABP125, ABP130, SEC31-like protein 1, SEC31-related protein A, Web1-like protein

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Sec31A antibody (AB253009)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Sec31A antibody (AB253009)

PFA-fixed, Triton X-100 permeabilized U-251MG (Human brain glioma cell line) cells stained for Sec31A (green) using ab253009 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009)

Paraffin-embedded human skeletal-muscle tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis. Very weak positivity in myocytes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009)

Paraffin-embedded human cerebral-cortex tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009)

Paraffin-embedded human liver tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009)

Paraffin-embedded human endometrium tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SEC31A aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O94979

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sec31A also known as Secretory 31 homolog A is an important component in the process of vesicle formation within cells. It has a molecular mass of about 137 kDa. This protein primarily localizes to the endoplasmic reticulum (ER) exit sites where it plays a role in the transport of proteins. It functions in close association with the COPII (coat protein II) vesicle coat complex which is necessary for transporting proteins from the ER to the Golgi apparatus.
Biological function summary

Sec31A participates in the export of properly folded proteins from the ER. As part of the COPII complex Sec31A works with other proteins like Sec13 to form a cage-like structure encapsulating the transport vesicle. This event is important for maintaining protein homeostasis and efficient cellular operations. Sec31A's involvement ensures that proteins reach their correct destinations impacting overall cell health.

Pathways

He protein has significant involvement in the ER-to-Golgi transport pathway. In this pathway features an important interaction between Sec31A and Sar1 a small GTPase that initiates COPII assembly. The function of Sec31A is critical here as it allows the progression of cargo through the secretory pathway. This pathway is integral to many cellular processes including the proper secretion of hormones and enzymes.

Alterations and dysfunction in Sec31A have been linked to heart-related conditions and neurological disorders. Mutations in this protein can result in faulty protein transport leading to an accumulation of misfolded proteins and stress in the ER. Additionally disruptions in Sec31A activity have shown connections with the accumulation of neurodegenerative disorders. The relationship between Sec31A and Sec13 could further elucidate understanding of these conditions due to their collaborative role in vesicle formation and transport.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (PubMed : 10788476). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity).
See full target information SEC31A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of clinical investigation 51:e13541 PubMed33797091

2021

Circ_0007142 downregulates miR-874-3p-mediated GDPD5 on colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqing Wang,Hongshu Chen,Xueling Wei
View all publications
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Product promise

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