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Rabbit Polyclonal Sec31A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEC31A aa 600-800.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Sec31A antibody (AB253009), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (AB253009), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SEC31A aa 600-800. The exact immunogen used to generate this antibody is proprietary information. Database link O94979

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested
Orangutan
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Target data

Function

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (PubMed:10788476). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal Sec31A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEC31A aa 600-800.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SEC31A aa 600-800. The exact immunogen used to generate this antibody is proprietary information. Database link O94979
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Sec31A also known as Secretory 31 homolog A is an important component in the process of vesicle formation within cells. It has a molecular mass of about 137 kDa. This protein primarily localizes to the endoplasmic reticulum (ER) exit sites where it plays a role in the transport of proteins. It functions in close association with the COPII (coat protein II) vesicle coat complex which is necessary for transporting proteins from the ER to the Golgi apparatus.

Biological function summary

Sec31A participates in the export of properly folded proteins from the ER. As part of the COPII complex Sec31A works with other proteins like Sec13 to form a cage-like structure encapsulating the transport vesicle. This event is important for maintaining protein homeostasis and efficient cellular operations. Sec31A's involvement ensures that proteins reach their correct destinations impacting overall cell health.

Pathways

He protein has significant involvement in the ER-to-Golgi transport pathway. In this pathway features an important interaction between Sec31A and Sar1 a small GTPase that initiates COPII assembly. The function of Sec31A is critical here as it allows the progression of cargo through the secretory pathway. This pathway is integral to many cellular processes including the proper secretion of hormones and enzymes.

Associated diseases and disorders

Alterations and dysfunction in Sec31A have been linked to heart-related conditions and neurological disorders. Mutations in this protein can result in faulty protein transport leading to an accumulation of misfolded proteins and stress in the ER. Additionally disruptions in Sec31A activity have shown connections with the accumulation of neurodegenerative disorders. The relationship between Sec31A and Sec13 could further elucidate understanding of these conditions due to their collaborative role in vesicle formation and transport.

Product promise

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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Sec31A antibody (ab253009), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sec31A antibody (ab253009)

    PFA-fixed, Triton X-100 permeabilized U-251MG (Human brain glioma cell line) cells stained for Sec31A (green) using ab253009 at 4 μg/ml in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (ab253009), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (ab253009)

    Paraffin-embedded human endometrium tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (ab253009), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (ab253009)

    Paraffin-embedded human liver tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (ab253009), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (ab253009)

    Paraffin-embedded human cerebral-cortex tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (ab253009), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec31A antibody (ab253009)

    Paraffin-embedded human skeletal-muscle tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis. Very weak positivity in myocytes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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