Rabbit Polyclonal Sec31A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEC31A aa 600-800.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Orangutan | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan | Dilution info - | Notes - |
Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (PubMed:10788476). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity).
KIAA0905, SEC31L1, HSPC275, HSPC334, SEC31A, Protein transport protein Sec31A, ABP125, ABP130, SEC31-like protein 1, SEC31-related protein A, Web1-like protein
Rabbit Polyclonal Sec31A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEC31A aa 600-800.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Sec31A also known as Secretory 31 homolog A is an important component in the process of vesicle formation within cells. It has a molecular mass of about 137 kDa. This protein primarily localizes to the endoplasmic reticulum (ER) exit sites where it plays a role in the transport of proteins. It functions in close association with the COPII (coat protein II) vesicle coat complex which is necessary for transporting proteins from the ER to the Golgi apparatus.
Sec31A participates in the export of properly folded proteins from the ER. As part of the COPII complex Sec31A works with other proteins like Sec13 to form a cage-like structure encapsulating the transport vesicle. This event is important for maintaining protein homeostasis and efficient cellular operations. Sec31A's involvement ensures that proteins reach their correct destinations impacting overall cell health.
He protein has significant involvement in the ER-to-Golgi transport pathway. In this pathway features an important interaction between Sec31A and Sar1 a small GTPase that initiates COPII assembly. The function of Sec31A is critical here as it allows the progression of cargo through the secretory pathway. This pathway is integral to many cellular processes including the proper secretion of hormones and enzymes.
Alterations and dysfunction in Sec31A have been linked to heart-related conditions and neurological disorders. Mutations in this protein can result in faulty protein transport leading to an accumulation of misfolded proteins and stress in the ER. Additionally disruptions in Sec31A activity have shown connections with the accumulation of neurodegenerative disorders. The relationship between Sec31A and Sec13 could further elucidate understanding of these conditions due to their collaborative role in vesicle formation and transport.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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PFA-fixed, Triton X-100 permeabilized U-251MG (Human brain glioma cell line) cells stained for Sec31A (green) using ab253009 at 4 μg/ml in ICC/IF.
Paraffin-embedded human endometrium tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral-cortex tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal-muscle tissue stained for Sec31A using ab253009 at 1/50 dilution in immunohistochemical analysis. Very weak positivity in myocytes.
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