AB86600

Anti-Sec31A antibody

Name: Anti-Sec31A antibody (ab86600) | Abcam
Brand: Abcam Limited
SKU: AB86600
Price: 480 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal Sec31A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SEC31A aa 750-850.

View Alternative Names

KIAA0905, SEC31L1, HSPC275, HSPC334, SEC31A, Protein transport protein Sec31A, ABP125, ABP130, SEC31-like protein 1, SEC31-related protein A, Web1-like protein

2 Images

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Immunoprecipitation - Anti-Sec31A antibody (AB86600)

Detection of Sec31A by Western Blot of Immunprecipitate.
ab86600 at 0.1µg/ml staining Sec31A in HeLa whole cell lysate immunoprecipitated using ab86600 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-Sec31A antibody (ab86600)

Predicted band size: 133 kDa

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Western blot - Anti-Sec31A antibody (AB86600)

All lanes:

Western blot - Anti-Sec31A antibody (ab86600) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 133 kDa

Observed band size: 133 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SEC31A aa 750-850. The exact immunogen used to generate this antibody is proprietary information.

O94979

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sec31A also known as Secretory 31 homolog A is an important component in the process of vesicle formation within cells. It has a molecular mass of about 137 kDa. This protein primarily localizes to the endoplasmic reticulum (ER) exit sites where it plays a role in the transport of proteins. It functions in close association with the COPII (coat protein II) vesicle coat complex which is necessary for transporting proteins from the ER to the Golgi apparatus.

Biological function summary

Sec31A participates in the export of properly folded proteins from the ER. As part of the COPII complex Sec31A works with other proteins like Sec13 to form a cage-like structure encapsulating the transport vesicle. This event is important for maintaining protein homeostasis and efficient cellular operations. Sec31A's involvement ensures that proteins reach their correct destinations impacting overall cell health.

Pathways

He protein has significant involvement in the ER-to-Golgi transport pathway. In this pathway features an important interaction between Sec31A and Sar1 a small GTPase that initiates COPII assembly. The function of Sec31A is critical here as it allows the progression of cargo through the secretory pathway. This pathway is integral to many cellular processes including the proper secretion of hormones and enzymes.

Alterations and dysfunction in Sec31A have been linked to heart-related conditions and neurological disorders. Mutations in this protein can result in faulty protein transport leading to an accumulation of misfolded proteins and stress in the ER. Additionally disruptions in Sec31A activity have shown connections with the accumulation of neurodegenerative disorders. The relationship between Sec31A and Sec13 could further elucidate understanding of these conditions due to their collaborative role in vesicle formation and transport.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (PubMed : 10788476). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity).

See full target information SEC31A

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Viruses 17: PubMed40431628

2025

The COPII Transport Complex Participates in HPV16 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia M Day,Cynthia D Thompson,Andrea S Weisberg,John T Schiller

International journal of biological sciences 20:3956-3971 PubMed39113702

2024

PKCα Induced the Generation of Extracellular Vesicles in Activated Platelets to Promote Breast Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Zhao,Huan Tian,Xiaona Zhao,Lan Lan,Huanhuan Liu,Yi Sun,Fengyan Yu

Nature communications 14:5685 PubMed37709746

2023

KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics.

Applications

Unspecified application

Species

Unspecified reactive species

Mai Abdel Mouti,Siwei Deng,Martin Pook,Jessica Malzahn,Aniko Rendek,Stefania Militi,Reshma Nibhani,Zahir Soonawalla,Udo Oppermann,Chang-Il Hwang,Siim Pauklin

Viruses 14: PubMed35458569

2022

Foot-and-Mouth Disease Virus 3A Hijacks Sar1 and Sec12 for ER Remodeling in a COPII-Independent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Heng-Wei Lee,Yi-Fan Jiang,Hui-Wen Chang,Ivan-Chen Cheng

Journal of lipid research 61:432-444 PubMed31806727

2019

Nonsynonymous SNPs in homologous to plasminogen deficiency mutants represent novel null apo(a) alleles.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin M Morgan,Aimee N Brown,Nikita Deo,Tom W R Harrop,George Taiaroa,Peter D Mace,Sigurd M Wilbanks,Tony R Merriman,Michael J A Williams,Sally P A McCormick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Sec31A antibody

Immunoprecipitation - Anti-Sec31A antibody (AB86600)

Western blot - Anti-Sec31A antibody (AB86600)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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