JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB240264

Anti-SEC61A antibody [EPR14379] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal SEC61A antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SEC61A, SEC61A1, Protein transport protein Sec61 subunit alpha isoform 1, Sec61 alpha-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61A antibody [EPR14379] - BSA and Azide free (AB240264)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61A antibody [EPR14379] - BSA and Azide free (AB240264)

Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling SEC61A with ab183046 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183046).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-SEC61A antibody [EPR14379] - BSA and Azide free (AB240264)
  • IP

Supplier Data

Immunoprecipitation - Anti-SEC61A antibody [EPR14379] - BSA and Azide free (AB240264)

Western blot analysis of SEC61A in Daudi cell lysate immunoprecipitated using ab183046 at 1/50 dilution (Lane 1). Lane 2 : Negative control.

Secondary antibody : Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG at 1/1500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183046).

All lanes:

Immunoprecipitation - Anti-SEC61A antibody [EPR14379] (<a href='/en-us/products/primary-antibodies/sec61a-antibody-epr14379-ab183046'>ab183046</a>)

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14379

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB1327

Anti-SEC61A antibody

Western blot - Anti-SEC61A antibody (AB1327)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB15576

Anti-SEC61B antibody

Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (AB15576)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB52649

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649)

  • 5
  • 39
Primary Antibodies

AB218624

Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB218624)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab240264 is the carrier-free version of ab183046.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC61A also known as Sec61p or protein transport protein Sec61 subunit alpha isoform 1 serves as an important component of the protein translocation process. This protein weighs approximately 54 kDa and is expressed in the endoplasmic reticulum (ER) membrane of eukaryotic cells. SEC61A forms part of the SEC61 complex which operates as a channel for polypeptide chains to enter the ER from the cytosol. It plays a central role in the translocation of nascent polypeptide chains into the ER or their integration into the ER membrane.
Biological function summary

The SEC61 complex holds significance in the translocon channel with SEC61A contributing to the determination of ER insertion or membrane integration of proteins. Its function is often associated with the ribosome as the complex forms a conduit for nascent proteins during translation. The SEC61A ensures proper protein localization with the translocon complex acting as a bridge between the ribosome and the ER. It is integral to cellular processes that require precise protein folding modification and targeting within cells.

Pathways

The role of SEC61A in protein targeting is vital for maintaining various cellular functions. The unfolded protein response (UPR) and the ER-associated degradation (ERAD) pathways depend on SEC61A for the recognition and resolution of misfolded proteins. In these pathways SEC61A interacts with the proteins like BIP and other chaperones involved in monitoring protein folding status and activating the necessary stress responses if unfolded proteins accumulate. Its involvement in these pathways highlights its importance in maintaining cellular homeostasis.

Mutations or dysfunctions of SEC61A contribute to various conditions such as cystic fibrosis and diabetes. Improper function of SEC61A links to defective protein translocation leading to accumulation of misfolded proteins a characteristic observed in cystic fibrosis alongside the CFTR protein. Similarly in diabetes inefficient SEC61A function might disturb insulin production or secretion since SEC61A's role in protein translocation and processing is fundamental. Moreover SEC61A dysfunction can affect proteins like IAPP involved in β-cell amyloid formation further linking it to diabetes pathogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER) (PubMed : 12475939, PubMed : 22375059, PubMed : 28782633, PubMed : 29719251, PubMed : 32814900). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides (PubMed : 22375059, PubMed : 28782633, PubMed : 29719251). May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins (PubMed : 22375059, PubMed : 29719251). The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins : it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex (PubMed : 32820719, PubMed : 36261522). The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER (PubMed : 8616892). Controls the passive efflux of calcium ions from the ER lumen to the cytosol through SEC61 channel, contributing to the maintenance of cellular calcium homeostasis (PubMed : 28782633). Plays a critical role in nephrogenesis, specifically at pronephros stage (By similarity).
See full target information SEC61A1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 187:3120-3140.e29 PubMed38714197

2024

Mapping the cellular biogeography of human bone marrow niches using single-cell transcriptomics and proteomic imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Shovik Bandyopadhyay,Michael P Duffy,Kyung Jin Ahn,Jonathan H Sussman,Minxing Pang,David Smith,Gwendolyn Duncan,Iris Zhang,Jeffrey Huang,Yulieh Lin,Barbara Xiong,Tamjid Imtiaz,Chia-Hui Chen,Anusha Thadi,Changya Chen,Jason Xu,Melissa Reichart,Zachary Martinez,Caroline Diorio,Chider Chen,Vinodh Pillai,Oraine Snaith,Derek Oldridge,Siddharth Bhattacharyya,Ivan Maillard,Martin Carroll,Charles Nelson,Ling Qin,Kai Tan
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com