Rabbit Polyclonal SEC61B antibody. Suitable for ICC/IF and reacts with Dog, Mouse, Rat, Human, Pig samples. Cited in 4 publications.
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- Basic research in cardiology 116:632021Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVladimir Bogdanov,Andrew M Soltisz,Nicolae Moise,Galina Sakuta,Benjamin Hernandez Orengo,Paul M L Janssen,Seth H Weinberg,Jonathan P Davis,Rengasayee Veeraraghavan,Sandor GyörkePubMed 34713358
- Cell research 31:141-1562020COPII mitigates ER stress by promoting formation of ER whorls.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Xu,Wanqing Du,Qin Zou,Yuting Wang,Xin Zhang,Xudong Xing,Ying Li,Dachuan Zhang,Huimin Wang,Wenhao Zhang,Xinyao Hu,Xin Liu,Xiaoling Liu,Shaojin Zhang,Jinqiang Yu,Jianhuo Fang,Fajin Li,Ying Zhou,Tieqiang Yue,Na Mi,Haiteng Deng,Peng Zou,Xiaowei Chen,Xuerui Yang,Li YuPubMed 32989223
- The Journal of cell biology 216:1301-13202017The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuezhao Liu,Yang Li,Xin Wang,Ruxiao Xing,Kai Liu,Qiwen Gan,Changyong Tang,Zhiyang Gao,Youli Jian,Shouqing Luo,Weixiang Guo,Chonglin YangPubMed 28404643
- Nature 535:164-82016A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Zhang et. al.PubMed 27383988
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Expected |
| Mouse | Expected |
| Rat | Expected |
| Dog | Tested |
| Pig | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER) (PubMed:12475939). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides (PubMed:12475939). The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex (PubMed:32820719, PubMed:36261522). The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER (PubMed:19121997).
Alternative names
Protein transport protein Sec61 subunit beta, SEC61B
Recommended products
Rabbit Polyclonal SEC61B antibody. Suitable for ICC/IF and reacts with Dog, Mouse, Rat, Human, Pig samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purity
- Whole antiserum
- Specificity
Ab15576 detects human, dog, pig, and rat sec61 beta from microsomes and shows no cross-reactivity with other sec61 isoforms.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEC61B also known as Sec61 beta subunit or Protein Transport Protein Sec61 subunit beta is a component of the Sec61 complex. It has a mass of approximately 9 kDa. SEC61B is expressed broadly in various tissues but it is found most abundantly in the endoplasmic reticulum (ER) membrane. This protein functions mechanically as a part of the protein translocation machinery that guides nascent polypeptides into or across the ER membrane.
- Biological function summary
SEC61B assists in the regulation and positioning of the Sec61 translocon complex which includes alpha and gamma subunits. This protein is not a gatekeeper of the channel but rather stabilizes the complex and influences co-translational and post-translational translocation of proteins. It plays roles in maintaining ER homeostasis and facilitating the integration of newly synthesized proteins into the ER membrane affecting various cellular processes such as secretion and membrane biogenesis.
- Pathways
SEC61B operates in the protein translocation pathways within the ER membrane. It is integral to the secretory pathway that moves proteins from the cytoplasm into the ER lumen or membrane. Additionally SEC61B interacts with proteins like ribosome-bound signal recognition particle (SRP) to facilitate accurate protein targeting and translocation. It associates with other ER resident proteins to manage polypeptide folding and processing.
- Associated diseases and disorders
SEC61B has connections to conditions that involve protein misfolding and improperly handled ER stress such as cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis misfolded proteins can trigger ER stress and cause adaptive responses. The involvement of proteins like the SEC61 complex in ameliorating ER stress highlights potential therapeutic targets. Therefore understanding SEC61B function and regulation may provide insights into therapeutic intervention for ER-stress-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (ab15576)
Immunofluorescence analysis of SEC61B was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SEC61B Rabbit Polyclonal Antibody (ab15576) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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