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AB244487

Anti-SEC61B antibody

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(1 Publication)

Rabbit Polyclonal SEC61B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEC61B aa 1 to C-terminus.

View Alternative Names

Protein transport protein Sec61 subunit beta, SEC61B

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (AB244487)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (AB244487)

PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for SEC61B (green) using ab244487 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)

Paraffin-embedded human cerebellum tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)

Paraffin-embedded human pancreas tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)

Paraffin-embedded human liver tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)

Paraffin-embedded human duodenum tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61B antibody (AB244487)

Paraffin-embedded human skeletal muscle tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis. No positivity in myocytes, as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SEC61B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P60468

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC61B also known as Sec61 beta subunit or Protein Transport Protein Sec61 subunit beta is a component of the Sec61 complex. It has a mass of approximately 9 kDa. SEC61B is expressed broadly in various tissues but it is found most abundantly in the endoplasmic reticulum (ER) membrane. This protein functions mechanically as a part of the protein translocation machinery that guides nascent polypeptides into or across the ER membrane.
Biological function summary

SEC61B assists in the regulation and positioning of the Sec61 translocon complex which includes alpha and gamma subunits. This protein is not a gatekeeper of the channel but rather stabilizes the complex and influences co-translational and post-translational translocation of proteins. It plays roles in maintaining ER homeostasis and facilitating the integration of newly synthesized proteins into the ER membrane affecting various cellular processes such as secretion and membrane biogenesis.

Pathways

SEC61B operates in the protein translocation pathways within the ER membrane. It is integral to the secretory pathway that moves proteins from the cytoplasm into the ER lumen or membrane. Additionally SEC61B interacts with proteins like ribosome-bound signal recognition particle (SRP) to facilitate accurate protein targeting and translocation. It associates with other ER resident proteins to manage polypeptide folding and processing.

SEC61B has connections to conditions that involve protein misfolding and improperly handled ER stress such as cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis misfolded proteins can trigger ER stress and cause adaptive responses. The involvement of proteins like the SEC61 complex in ameliorating ER stress highlights potential therapeutic targets. Therefore understanding SEC61B function and regulation may provide insights into therapeutic intervention for ER-stress-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER) (PubMed : 12475939). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides (PubMed : 12475939). The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins : it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex (PubMed : 32820719, PubMed : 36261522). The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER (PubMed : 19121997).
See full target information SEC61B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 19:344 PubMed34376200

2021

Heparin-binding growth factor (HDGF) drives radioresistance in breast cancer by activating the STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Qiu,Yan Ma,Xiaohua Chen,Liheng Zhou,Haibo Zhang,Guansheng Zhong,Lei Zhang,Jianming Tang
View all publications
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Product promise

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