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AB240567

Anti-SEC63 antibody

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(1 Publication)

Rabbit Polyclonal SEC63 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SEC63 aa 700-750.

View Alternative Names

DNAJC23, SEC63L, SEC63, Translocation protein SEC63 homolog, DnaJ homolog subfamily C member 23

2 Images
Immunoprecipitation - Anti-SEC63 antibody (AB240567)
  • IP

Supplier Data

Immunoprecipitation - Anti-SEC63 antibody (AB240567)

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
Lane 1 : ab240567 used for IP at 6 μg per reaction.
Lane 2 : Control IgG.

For WB ab240567 was used at 0.4 μg/ml.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-SEC63 antibody (ab240567)

Predicted band size: 88 kDa

false

Western blot - Anti-SEC63 antibody (AB240567)
  • WB

Supplier Data

Western blot - Anti-SEC63 antibody (AB240567)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-SEC63 antibody (ab240567) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 88 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SEC63 aa 700-750. The exact immunogen used to generate this antibody is proprietary information.

Q9UGP8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/25000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/25000", "WB-species-notes": "<p></p>" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab240567 was affinity purified using an epitope specific to SEC63 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SEC63 protein also known as NNF1 plays a critical role in the process of protein translocation across the endoplasmic reticulum (ER) membrane. It has a molecular mass of approximately 80 kDa. SEC63 is widely expressed in various tissues with notable expression in the ER where it functions as part of the translocon complex facilitating the proper insertion and folding of nascent polypeptides into the ER. This protein contains several distinct domains that contribute to its function in protein trafficking and biogenesis within the cell.
Biological function summary

SEC63 participates in cooperation with other components of the Sec61 translocon complex to assist in the translocation of polypeptides from the cytosol into the ER. It is essential for the proper assembly of membrane and secretory proteins directly impacting protein maturation. SEC63 interacts with proteins such as SEC62 and BIP which further stabilize the translocon and assist in the unfolding and refolding of polypeptides. Through this complex SEC63 plays an integral role in maintaining ER homeostasis.

Pathways

SEC63 is an integral component of the protein secretion pathway and the ER-associated degradation (ERAD) pathway. In the protein secretion pathway SEC63 cooperates with proteins like SEC61 to facilitate the proper folding and assembly of proteins destined for secretion. In the ERAD pathway SEC63 aids in recognizing and targeting misfolded proteins for degradation thereby preventing accumulation of defective proteins. By participating in these pathways SEC63 ensures the fidelity of protein quality control and homeostasis within the cellular environment.

Mutations in SEC63 have been linked to polycystic liver disease (PCLD) and can also influence processes related to cancer development. In PCLD the SEC63 protein's impaired function leads to defective protein processing contributing to cyst formation in liver tissue. SEC63's involvement in cancer relates to its role in maintaining protein quality control where disruption can result in an environment conducive to tumor growth. The interaction between SEC63 and proteins such as polycystin-1 and polycystin-2 highlights its importance in these pathological conditions as these proteins are similarly implicated in such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates cotranslational and post-translational transport of certain precursor polypeptides across endoplasmic reticulum (ER) (PubMed : 22375059, PubMed : 29719251). Proposed to play an auxiliary role in recognition of precursors with short and apolar signal peptides. May cooperate with SEC62 and HSPA5/BiP to facilitate targeting of small presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen (PubMed : 29719251). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (By similarity).
See full target information SEC63
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 637:983-990 PubMed39663456

2024

In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor.

Applications

Unspecified application

Species

Unspecified reactive species

Huaipeng Xing,Remus R E Rosenkranz,Piere Rodriguez-Aliaga,Ting-Ting Lee,Tomáš Majtner,Stefanie Böhm,Beata Turoňová,Judith Frydman,Martin Beck
View all publications
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