AB244497

Anti-SEC63 antibody

Name: Anti-SEC63 antibody (ab244497) | Abcam
Brand: Abcam Limited
SKU: AB244497
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SEC63 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEC63 aa 50-200.

View Alternative Names

DNAJC23, SEC63L, SEC63, Translocation protein SEC63 homolog, DnaJ homolog subfamily C member 23

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-SEC63 antibody (AB244497)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SEC63 antibody (AB244497)

PFA-fixed, Triton X-100 permeabilized Hep G2 cells stained for SEC63 (green) using ab244497 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Paraffin-embedded human testis tissue stained for SEC63 using ab244497 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Paraffin-embedded human skeletal muscle tissue stained for SEC63 using ab244497 at 1/50 dilution in immunohistochemical analysis. No positivity observed.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Paraffin-embedded human pancreas tissue stained for SEC63 using ab244497 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Paraffin-embedded human salivary gland tissue stained for SEC63 using ab244497 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-SEC63 antibody (AB244497)

All lanes:

Western blot - Anti-SEC63 antibody (ab244497) at 0.4 µg/mL

All lanes:

SK-MEL-30 (human metastatic malignant melanoma cell line) cell lysate

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SEC63 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9UGP8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SEC63 protein also known as NNF1 plays a critical role in the process of protein translocation across the endoplasmic reticulum (ER) membrane. It has a molecular mass of approximately 80 kDa. SEC63 is widely expressed in various tissues with notable expression in the ER where it functions as part of the translocon complex facilitating the proper insertion and folding of nascent polypeptides into the ER. This protein contains several distinct domains that contribute to its function in protein trafficking and biogenesis within the cell.

Biological function summary

SEC63 participates in cooperation with other components of the Sec61 translocon complex to assist in the translocation of polypeptides from the cytosol into the ER. It is essential for the proper assembly of membrane and secretory proteins directly impacting protein maturation. SEC63 interacts with proteins such as SEC62 and BIP which further stabilize the translocon and assist in the unfolding and refolding of polypeptides. Through this complex SEC63 plays an integral role in maintaining ER homeostasis.

Pathways

SEC63 is an integral component of the protein secretion pathway and the ER-associated degradation (ERAD) pathway. In the protein secretion pathway SEC63 cooperates with proteins like SEC61 to facilitate the proper folding and assembly of proteins destined for secretion. In the ERAD pathway SEC63 aids in recognizing and targeting misfolded proteins for degradation thereby preventing accumulation of defective proteins. By participating in these pathways SEC63 ensures the fidelity of protein quality control and homeostasis within the cellular environment.

Mutations in SEC63 have been linked to polycystic liver disease (PCLD) and can also influence processes related to cancer development. In PCLD the SEC63 protein's impaired function leads to defective protein processing contributing to cyst formation in liver tissue. SEC63's involvement in cancer relates to its role in maintaining protein quality control where disruption can result in an environment conducive to tumor growth. The interaction between SEC63 and proteins such as polycystin-1 and polycystin-2 highlights its importance in these pathological conditions as these proteins are similarly implicated in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Mediates cotranslational and post-translational transport of certain precursor polypeptides across endoplasmic reticulum (ER) (PubMed : 22375059, PubMed : 29719251). Proposed to play an auxiliary role in recognition of precursors with short and apolar signal peptides. May cooperate with SEC62 and HSPA5/BiP to facilitate targeting of small presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen (PubMed : 29719251). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (By similarity).

See full target information SEC63

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:5786-5809 PubMed39308676

2024

Prognostic and Immunological Role of Cuproptosis-Related Gene MTF1 in Pan-Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhang,Sifen Wang,Hailin Tang,Renchun Lai,Qiaoting Cai,Yaorong Su,Hao Wu,Yongwen Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEC63 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SEC63 antibody (AB244497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Western blot - Anti-SEC63 antibody (AB244497)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise