AB68550

Anti-SEC63 antibody

Name: Anti-SEC63 antibody (ab68550) | Abcam
Brand: Abcam Limited
SKU: AB68550
Price: 535 USD
Availability: InStock

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(2 Publications)

Mouse Polyclonal SEC63 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SEC63.

View Alternative Names

DNAJC23, SEC63L, SEC63, Translocation protein SEC63 homolog, DnaJ homolog subfamily C member 23

2 Images

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Western blot - Anti-SEC63 antibody (AB68550)

All lanes:

Western blot - Anti-SEC63 antibody (ab68550) at 1/500 dilution

All lanes:

Human pancreas lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L) at 1/2500 dilution

Predicted band size: 88 kDa,94 kDa

Observed band size: 88 kDa,90 kDa

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Western blot - Anti-SEC63 antibody (AB68550)

All lanes:

Western blot - Anti-SEC63 antibody (ab68550) at 1/500 dilution

Lane 1:

SEC63 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L) at 1/2500 dilution

Predicted band size: 88 kDa

Observed band size: 88 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SEC63.

Q9UGP8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SEC63 protein also known as NNF1 plays a critical role in the process of protein translocation across the endoplasmic reticulum (ER) membrane. It has a molecular mass of approximately 80 kDa. SEC63 is widely expressed in various tissues with notable expression in the ER where it functions as part of the translocon complex facilitating the proper insertion and folding of nascent polypeptides into the ER. This protein contains several distinct domains that contribute to its function in protein trafficking and biogenesis within the cell.

Biological function summary

SEC63 participates in cooperation with other components of the Sec61 translocon complex to assist in the translocation of polypeptides from the cytosol into the ER. It is essential for the proper assembly of membrane and secretory proteins directly impacting protein maturation. SEC63 interacts with proteins such as SEC62 and BIP which further stabilize the translocon and assist in the unfolding and refolding of polypeptides. Through this complex SEC63 plays an integral role in maintaining ER homeostasis.

Pathways

SEC63 is an integral component of the protein secretion pathway and the ER-associated degradation (ERAD) pathway. In the protein secretion pathway SEC63 cooperates with proteins like SEC61 to facilitate the proper folding and assembly of proteins destined for secretion. In the ERAD pathway SEC63 aids in recognizing and targeting misfolded proteins for degradation thereby preventing accumulation of defective proteins. By participating in these pathways SEC63 ensures the fidelity of protein quality control and homeostasis within the cellular environment.

Mutations in SEC63 have been linked to polycystic liver disease (PCLD) and can also influence processes related to cancer development. In PCLD the SEC63 protein's impaired function leads to defective protein processing contributing to cyst formation in liver tissue. SEC63's involvement in cancer relates to its role in maintaining protein quality control where disruption can result in an environment conducive to tumor growth. The interaction between SEC63 and proteins such as polycystin-1 and polycystin-2 highlights its importance in these pathological conditions as these proteins are similarly implicated in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates cotranslational and post-translational transport of certain precursor polypeptides across endoplasmic reticulum (ER) (PubMed : 22375059, PubMed : 29719251). Proposed to play an auxiliary role in recognition of precursors with short and apolar signal peptides. May cooperate with SEC62 and HSPA5/BiP to facilitate targeting of small presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen (PubMed : 29719251). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (By similarity).

See full target information SEC63

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e18261 PubMed38526029

2024

Depletion of CPNE7 sensitizes colorectal cancer to 5-fluorouracil by downregulating ATG9B expression.

Applications

Unspecified application

Species

Unspecified reactive species

Weile Xu,Yujie Tang,Yang Yang,Changjing Wang,Chen Liu,Jianqing Zhang,Lianmei Zhao,Guiying Wang

Biochemistry 59:2299-2311 PubMed32462874

2020

Mapping the Neuroanatomy of ABHD16A, ABHD12, and Lysophosphatidylserines Provides New Insights into the Pathophysiology of the Human Neurological Disorder PHARC.

Applications

Unspecified application

Species

Unspecified reactive species

Shubham Singh,Alaumy Joshi,Siddhesh S Kamat

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SEC63 antibody

Western blot - Anti-SEC63 antibody (AB68550)

Western blot - Anti-SEC63 antibody (AB68550)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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