Rabbit Polyclonal Sec8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EXOC4 aa 800 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EXOC4 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96A65
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Alternative names
Exocyst complex component 4, Exocyst complex component Sec8, SEC8L1, SEC8, KIAA1699, EXOC4
Recommended products
Rabbit Polyclonal Sec8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EXOC4 aa 800 to C-terminus.
Alternative names
Exocyst complex component 4, Exocyst complex component Sec8, SEC8L1, SEC8, KIAA1699, EXOC4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EXOC4 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96A65
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sec8 also known as Exocyst complex component 4 (EXOC4) is a protein with a mass of approximately 100 kDa. It acts as a critical component of the exocyst complex which is essential for the tethering of vesicles to specific sites on the plasma membrane. Sec8 shows high expression in a variety of human tissues including the brain kidney and intestine. By anchoring vesicles it plays an important role in exocytosis and the regulation of membrane dynamics.
- Biological function summary
The Sec8 protein contributes to cellular events by serving as a part of the exocyst complex a multiprotein assembly involved in vesicle trafficking. This complex consists of eight proteins including Sec3 Exo70 and Sec15. The assembly coordinates precise vesicle delivery facilitating processes such as cell growth migration and polarity establishment. Its activity is important for the proper functioning of secretory pathways ensuring communication and material transport across cell membranes.
- Pathways
Sec8 operates within the secretory pathway that manages delivery and distribution of materials inside cells. It is also involved in the sustainable function of the polarized secretion pathway an essential process that ensures cellular asymmetry and directional growth. Sec8's interaction with proteins like Rab family GTPases is significant in these pathways which are responsible for guiding vesicle movement and anchoring to the target membrane.
- Associated diseases and disorders
Sec8 associations have been identified in relation to cancer and impaired cognitive function. Alterations in the expression or function of Sec8 may disrupt vesicle trafficking leading to tumorigenesis or neurodevelopmental disorders. For example within cancer this disruption can affect cell adhesion and migration promoting metastasis. Additionally interactions with proteins such as Sec6 another exocyst complex member further illustrate the interconnected impact of Sec8 dysregulation on diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-Sec8 antibody (ab224484)
All lanes: Western blot - Anti-Sec8 antibody (ab224484) at 1/100 dilution
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG sp (human brain glioma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 110 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec8 antibody (ab224484)
Paraffin-embedded human duodenum tissue stained for Sec8 using ab224484 at 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Sec8 antibody (ab224484)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Sec8 (green) using ab224484 at 4 μg/ml in ICC/IF.
Downloads
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