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AB224484

Anti-Sec8 antibody

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(1 Publication)

Rabbit Polyclonal Sec8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EXOC4 aa 800 to C-terminus.

View Alternative Names

KIAA1699, SEC8, SEC8L1, EXOC4, Exocyst complex component 4, Exocyst complex component Sec8

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec8 antibody (AB224484)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec8 antibody (AB224484)

Paraffin-embedded human duodenum tissue stained for Sec8 using ab224484 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Sec8 antibody (AB224484)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sec8 antibody (AB224484)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Sec8 (green) using ab224484 at 4 μg/ml in ICC/IF.

Western blot - Anti-Sec8 antibody (AB224484)
  • WB

Supplier Data

Western blot - Anti-Sec8 antibody (AB224484)

All lanes:

Western blot - Anti-Sec8 antibody (ab224484) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Predicted band size: 110 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EXOC4 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96A65

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Alternative Products

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AB205945

Anti-Sec8 antibody - C-terminal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sec8 also known as Exocyst complex component 4 (EXOC4) is a protein with a mass of approximately 100 kDa. It acts as a critical component of the exocyst complex which is essential for the tethering of vesicles to specific sites on the plasma membrane. Sec8 shows high expression in a variety of human tissues including the brain kidney and intestine. By anchoring vesicles it plays an important role in exocytosis and the regulation of membrane dynamics.
Biological function summary

The Sec8 protein contributes to cellular events by serving as a part of the exocyst complex a multiprotein assembly involved in vesicle trafficking. This complex consists of eight proteins including Sec3 Exo70 and Sec15. The assembly coordinates precise vesicle delivery facilitating processes such as cell growth migration and polarity establishment. Its activity is important for the proper functioning of secretory pathways ensuring communication and material transport across cell membranes.

Pathways

Sec8 operates within the secretory pathway that manages delivery and distribution of materials inside cells. It is also involved in the sustainable function of the polarized secretion pathway an essential process that ensures cellular asymmetry and directional growth. Sec8's interaction with proteins like Rab family GTPases is significant in these pathways which are responsible for guiding vesicle movement and anchoring to the target membrane.

Sec8 associations have been identified in relation to cancer and impaired cognitive function. Alterations in the expression or function of Sec8 may disrupt vesicle trafficking leading to tumorigenesis or neurodevelopmental disorders. For example within cancer this disruption can affect cell adhesion and migration promoting metastasis. Additionally interactions with proteins such as Sec6 another exocyst complex member further illustrate the interconnected impact of Sec8 dysregulation on diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
See full target information EXOC4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:5114 PubMed33037205

2020

Fgf and Wnt signaling interaction in the mesenchymal niche regulates the murine hair cycle clock.

Applications

Unspecified application

Species

Unspecified reactive species

Sarina Harshuk-Shabso,Hila Dressler,Christof Niehrs,Emil Aamar,David Enshell-Seijffers
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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