AB205945

Anti-Sec8 antibody - C-terminal

Name: Anti-Sec8 antibody - C-terminal (ab205945) | Abcam
Brand: Abcam Limited
SKU: AB205945
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Sec8 antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EXOC4 aa 900-1000.

View Alternative Names

KIAA1699, SEC8, SEC8L1, EXOC4, Exocyst complex component 4, Exocyst complex component Sec8

2 Images

Supplier Data

Immunoprecipitation - Anti-Sec8 antibody - C-terminal (AB205945)

Lane 1 : Detection of Human Sec8 by Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab205945 at 6 μg for IP.
Lane 2 : Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab205945 at 1 μg/ml.

All lanes:

Immunoprecipitation - Anti-Sec8 antibody - C-terminal (ab205945)

Predicted band size: 110 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-Sec8 antibody - C-terminal (AB205945)

All lanes:

Western blot - Anti-Sec8 antibody - C-terminal (ab205945) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from 293T cells at 50 µg

Lane 3:

Whole cell lysate from Jurkat cells at 50 µg

Lane 4:

Whole cell lysate from mouse TCMK-1 cells at 50 µg

Lane 5:

Whole cell lysate from mouse NIH3T3 cells at 50 µg

Predicted band size: 110 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EXOC4 aa 900-1000. The exact immunogen used to generate this antibody is proprietary information.

Q96A65

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sec8 also known as Exocyst complex component 4 (EXOC4) is a protein with a mass of approximately 100 kDa. It acts as a critical component of the exocyst complex which is essential for the tethering of vesicles to specific sites on the plasma membrane. Sec8 shows high expression in a variety of human tissues including the brain kidney and intestine. By anchoring vesicles it plays an important role in exocytosis and the regulation of membrane dynamics.

Biological function summary

The Sec8 protein contributes to cellular events by serving as a part of the exocyst complex a multiprotein assembly involved in vesicle trafficking. This complex consists of eight proteins including Sec3 Exo70 and Sec15. The assembly coordinates precise vesicle delivery facilitating processes such as cell growth migration and polarity establishment. Its activity is important for the proper functioning of secretory pathways ensuring communication and material transport across cell membranes.

Pathways

Sec8 operates within the secretory pathway that manages delivery and distribution of materials inside cells. It is also involved in the sustainable function of the polarized secretion pathway an essential process that ensures cellular asymmetry and directional growth. Sec8's interaction with proteins like Rab family GTPases is significant in these pathways which are responsible for guiding vesicle movement and anchoring to the target membrane.

Sec8 associations have been identified in relation to cancer and impaired cognitive function. Alterations in the expression or function of Sec8 may disrupt vesicle trafficking leading to tumorigenesis or neurodevelopmental disorders. For example within cancer this disruption can affect cell adhesion and migration promoting metastasis. Additionally interactions with proteins such as Sec6 another exocyst complex member further illustrate the interconnected impact of Sec8 dysregulation on diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

See full target information EXOC4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 11:683915 PubMed34976784

2021

Development and Validation of an Immune-Related Signature for the Prediction of Recurrence Risk of Patients With Laryngeal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhang,Xudong Zhao,Jin Wang,Wenyue Ji

PLoS genetics 17:e1009285 PubMed33513160

2021

Genome-scale CRISPR screening for modifiers of cellular LDL uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Brian T Emmer,Emily J Sherman,Paul J Lascuna,Sarah E Graham,Cristen J Willer,David Ginsburg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sec8 antibody - C-terminal

Immunoprecipitation - Anti-Sec8 antibody - C-terminal (AB205945)

Western blot - Anti-Sec8 antibody - C-terminal (AB205945)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise