Rabbit Polyclonal Secretin receptor/SCTR antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Secretin receptor aa 1-150.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Secretin receptor aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P47872
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Target data
Function
Receptor for secretin (SCT), which is involved in different processes such as regulation of the pH of the duodenal content, food intake and water homeostasis (PubMed:25332973, PubMed:7612008). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity). Upon binding to secretin, regulates the pH of the duodenum by (1) inhibiting the secretion of gastric acid from the parietal cells of the stomach and (2) stimulating the production of bicarbonate (NaHCO(3)) from the ductal cells of the pancreas (By similarity). In addition to regulating the pH of the duodenal content, plays a central role in diet induced thermogenesis: acts as a non-sympathetic brown fat (BAT) activator mediating prandial thermogenesis, which consequentially induces satiation. Mechanistically, secretin released by the gut after a meal binds to secretin receptor (SCTR) in brown adipocytes, activating brown fat thermogenesis by stimulating lipolysis, which is sensed in the brain and promotes satiation. Also able to stimulate lipolysis in white adipocytes. Also plays an important role in cellular osmoregulation by regulating renal water reabsorption. Also plays a role in the central nervous system: required for synaptic plasticity (By similarity).
Alternative names
Secretin receptor, SCT-R, SCTR
Recommended products
Rabbit Polyclonal Secretin receptor/SCTR antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Secretin receptor aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Secretin receptor aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P47872
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Secretin receptor (SCTR) also known as the secretin R is a G protein-coupled receptor (GPCR) with a typical seven-transmembrane domain structure. It weighs around 52 kDa and primarily facilitates the action of secretin a hormone involved in various physiological functions. The receptor is expressed in multiple body tissues such as the pancreas stomach and certain regions of the brain. This distribution allows it to mediate the diverse roles of secretin as part of both local and systemic responses.
- Biological function summary
The Secretin receptor plays an integral role in regulating fluid and electrolyte balance as well as modulating gastric acid secretion. It does not form part of a complex but operates in concert with other receptors that shape digestive processes. When secretin binds to SCTR it initiates cellular responses that influence pancreatic bicarbonate secretion which is essential for neutralizing stomach acid and creating an optimal environment for intestinal enzymes.
- Pathways
The Secretin receptor is chiefly involved in the cAMP signaling pathway. This pathway engages adenylyl cyclase and elevates cAMP levels leading to protein kinase A (PKA) activation. SCTR works alongside other receptors such as the vasoactive intestinal peptide receptor (VIPR) to regulate these processes. This coordinated interaction drives cellular outcomes like enzyme secretion and motility within gastrointestinal tissues.
- Associated diseases and disorders
Alterations in SCTR function have been linked to pancreatic adenocarcinoma and congenital diarrhea syndromes. In these conditions receptor activity may affect signaling pathways and disrupt normal physiological balances. Research indicates that other proteins such as CFTR (cystic fibrosis transmembrane conductance regulator) align with SCTR function particularly in diseases involving disrupted fluid secretion and ion transport. Understanding these connections can help in developing targeted therapies and enhancing our grasp of gastrointestinal disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Secretin receptor/SCTR antibody (ab224236)
Paraffin-embedded human pancreas stained for SCTR using ab224236 at 1/50 dilution in immunohistochemical analysis. Shows strong membranous positivity in interlobular cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Secretin receptor/SCTR antibody (ab224236)
Paraffin-embedded human skeletal muscle stained for SCTR using ab224236 at 1/50 dilution in immunohistochemical analysis. Shows no positivity in myocytes as expected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Secretin receptor/SCTR antibody (ab224236)
Paraffin-embedded human skin stained for SCTR using ab224236 at 1/50 dilution in immunohistochemical analysis. Shows strong membranous positivity in squamous epithelial cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Secretin receptor/SCTR antibody (ab224236)
Paraffin-embedded human stomach stained for SCTR using ab224236 at 1/50 dilution in immunohistochemical analysis. Shows strong membranous positivity in glandular cells.
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