Rabbit Polyclonal Secretin/SCT antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Secretin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human Secretin. Database link P09683
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
Target data
Function
Hormone involved in different processes, such as regulation of the pH of the duodenal content, food intake and water homeostasis (PubMed:25332973). Exerts its biological effects by binding to secretin receptor (SCTR), a G-protein coupled receptor expressed in the basolateral domain of several cells (PubMed:25332973). Acts as a key gastrointestinal hormone by regulating the pH of the duodenal content (By similarity). Secreted by S cells of the duodenum in the crypts of Lieberkuehn and regulates the pH of the duodenum by (1) inhibiting the secretion of gastric acid from the parietal cells of the stomach and (2) stimulating the production of bicarbonate (NaHCO(3)) from the ductal cells of the pancreas (By similarity). Production of bicarbonate is essential to neutralize the pH and ensure no damage is done to the small intestine by the gastric acid (By similarity). In addition to regulating the pH of the duodenal content, plays a central role in diet induced thermogenesis: acts as a non-sympathetic brown fat (BAT) activator mediating prandial thermogenesis, which consequentially induces satiation (Probable). Mechanistically, secretin released by the gut after a meal binds to secretin receptor (SCTR) in brown adipocytes, activating brown fat thermogenesis by stimulating lipolysis, which is sensed in the brain and promotes satiation (By similarity). Also able to stimulate lipolysis in white adipocytes (By similarity). Also plays an important role in cellular osmoregulation: released into the systemic circulation in response to hyperosmolality and acts at different levels in the hypothalamus, pituitary and kidney to regulate water homeostasis (By similarity). Also plays a role in the central nervous system, possibly by acting as a neuropeptide hormone: required for hippocampal synaptic function and neural progenitor cells maintenance (By similarity).
Alternative names
Secretin, SCT
Recommended products
Rabbit Polyclonal Secretin/SCT antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Secretin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human Secretin. Database link P09683
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Secretin also known as SCT is a peptide hormone composed of 27 amino acids with a molecular mass of approximately 3.3 kDa. It is primarily expressed in the S cells of the duodenum in the small intestine. Secretin is also found in smaller quantities in the brain and other parts of the gastrointestinal tract. This hormone plays a role in stimulating the secretion of bicarbonate from the pancreas which helps neutralize stomach acid in the small intestine.
- Biological function summary
Secretin plays a significant role in the regulation of water homeostasis throughout the body. It coordinates the regulation of gastric acid secretion and pancreatic bicarbonate secretion helping in digestion. Secretin cannot be classified as part of a specific complex but it functions in conjunction with other digestive hormones like cholecystokinin. These hormones work together to regulate the overall digestive process and nutrient absorption.
- Pathways
Secretin engages primarily in the cAMP signaling pathway stimulating the production of cyclic AMP when binding to the secretin receptor. This action ultimately affects the release of pancreatic juices and bile essential for digestion. The secretin receptor is a member of the G-protein coupled receptor family closely related to other receptors like those for vasoactive intestinal peptide (VIP) and glucagon which mediate similar signaling cascades for gastrointestinal function.
- Associated diseases and disorders
Secretin has been associated with certain conditions like Zollinger-Ellison syndrome and pancreatic exocrine insufficiency. In Zollinger-Ellison syndrome tumors cause overproduction of gastric acid and secretin can be essential in managing the effects as it regulates gastric and pancreatic secretions. Furthermore secretin interacts with proteins like vasoactive intestinal peptide when assessing pancreatic function. Understanding these interactions is important for developing therapies targeting pancreatic and gastric disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Secretin/SCT antibody (ab103619)
All lanes: Western blot - Anti-Secretin/SCT antibody (ab103619) at 1/500 dilution
All lanes: HepG2 cell lysate at 50 µg
Predicted band size: 13 kDa, 15 kDa, 16 kDa, 17 kDa
Western blot - Anti-Secretin/SCT antibody (ab103619)
All lanes: Western blot - Anti-Secretin/SCT antibody (ab103619) at 1/500 dilution
Lane 1: Secretin/SCT transfected 293T cell lysate at 25 µg
Lane 2: Non-transfected 293T cell lysate at 25 µg
Predicted band size: 13 kDa
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