Name: Anti-Securin antibody [EPR3240]
SKU: ab79546
Rating: 4 (3 reviews)

Rabbit Recombinant Monoclonal Securin antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 28 publications.

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Images

Western blot - Anti-Securin antibody [EPR3240] (AB79546), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/5000 - 1/20000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes For unpurified use at 1/100 - 1/250.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target data

See full target information PTTG1

Function

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

Alternative names

EAP1, PTTG, TUTR1, PTTG1, Securin, Esp1-associated protein, Pituitary tumor-transforming gene 1 protein, Tumor-transforming protein 1, hPTTG

Recommended products

Rabbit Recombinant Monoclonal Securin antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 28 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR3240
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Securin also known as PTTG1 (pituitary tumor-transforming gene 1) is a protein involved in the regulation of sister chromatid separation during cell division. It inhibits Separase an enzyme important for this separation. Securin has a molecular mass of approximately 22 kDa. Its expression is notable in various tissues including the pituitary gland and cancerous tissues which suggests a role in tumorigenesis.

Biological function summary

Securing proper chromosome segregation Securin forms a complex with Separase. By inhibiting Separase Securin ensures that separation of chromatids occurs only at the correct stage of mitosis. This regulatory function is critical for maintaining genomic stability and avoiding aneuploidy which can lead to tumorigenesis. The presence of Securin in dividing cells highlights its role in the cell cycle.

Pathways

Securin functions are integrated within the cell cycle and apoptosis pathways. In the cell cycle pathway Securin controls the metaphase-anaphase transition. This involves interaction with cyclin-dependent kinases (CDKs) and the anaphase-promoting complex (APC/C). In terms of apoptosis Securin may interact with proteins like Bcl-2 impacting cell survival and death decisions under stress conditions.

Associated diseases and disorders

Securin has been associated with cancer particularly pituitary adenomas and colorectal cancer. Its overexpression can lead to chromosomal instability a hallmark of many cancers. Furthermore interactions with other proteins like p53 known for its tumor-suppressing activities suggest a complex interplay in oncogenic processes where disruptions can contribute to tumor development and progression.

Product promise

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Full details and terms and conditions can be found here:
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8 product images

Western blot - Anti-Securin antibody [EPR3240] (ab79546)
Lanes 1-3: Merged signal (red and green). Green - ab79546 observed at 28 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
ab79546 Anti-Securin antibody [EPR3240] was shown to specifically react with Securin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human PTTG1 (Securin) knockout HEK-293T cell line ab266231 (knockout cell lysate Human PTTG1 (Securin) knockout HEK-293T cell lysate ab257289) was used. Wild-type and Securin knockout samples were subjected to SDS-PAGE. ab79546 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Securin antibody [EPR3240] (ab79546) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: PTTG1 knockout HEK293T cell lysate at 20 µg
Lane 3: Daudi cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 28 kDa
Western blot - Anti-Securin antibody [EPR3240] (ab79546)
All lanes: Western blot - Anti-Securin antibody [EPR3240] (ab79546) at 1/1000 dilution
Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates at 15 µg
Lane 2: HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 28 kDa
Flow Cytometry (Intracellular) - Anti-Securin antibody [EPR3240] (ab79546)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Securin with purified ab79546 at 1/30 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Securin antibody [EPR3240] (ab79546)
All lanes: Western blot - Anti-Securin antibody [EPR3240] (ab79546) at 1/20000 dilution
Lane 1: Daudi (Human Burkitt's lymphoma cell line) cell lysate at 10 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa, 28 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Securin antibody [EPR3240] (ab79546)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Securin with purified ab79546 at 1/50 dilution (4.74 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain.
PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Securin antibody [EPR3240] (ab79546)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Securin with purified ab79546 at 1/200 dilution (1.19 μg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Securin antibody [EPR3240] (ab79546)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling securin with unpurified ab79546 at 1/250 dilution. The cells were permeabilised with 0.1% Triton X-100. Anti-rabbit Alexa Fluor^® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).
Flow Cytometry (Intracellular) - Anti-Securin antibody [EPR3240] (ab79546)
Intracellular flow cytometric analysis of permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells using unpurified ab79546 (red) or a rabbit IgG (negative) (green).