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AB218531

Anti-SEH1L antibody [EPR20851]

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(11 Publications)

Rabbit Recombinant Monoclonal SEH1L antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 11 publications.

View Alternative Names

SEC13L, SEH1, SEH1L, Nucleoporin SEH1, GATOR2 complex protein SEH1, Nup107-160 subcomplex subunit SEH1, SEC13-like protein

3 Images
Immunoprecipitation - Anti-SEH1L antibody [EPR20851] (AB218531)
  • IP

Supplier Data

Immunoprecipitation - Anti-SEH1L antibody [EPR20851] (AB218531)

SEH1L was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate with ab218531 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218531 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1 : HEK-293T whole cell lysate 10 μg (Input).
Lane 2 : ab218531 IP in HEK-293T whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab218531 in HEK-293T whole cell lysate (-).
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 10 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID : 28199315).

According to the data of WB1, the band above 250 kDa could be protein aggregates containing SEH1L.

All lanes:

Immunoprecipitation - Anti-SEH1L antibody [EPR20851] (ab218531)

Predicted band size: 40 kDa

false

Western blot - Anti-SEH1L antibody [EPR20851] (AB218531)
  • WB

Supplier Data

Western blot - Anti-SEH1L antibody [EPR20851] (AB218531)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID : 28199315). The band above 250 kDa was also decreased in the SEH1L knockdown lysate. This suggests that the band contains SEH1L, possibly in protein aggregates.

The cell lysates were kindly provided by our collaborator Dr. Liang Zhang, Xiamen University.

All lanes:

Western blot - Anti-SEH1L antibody [EPR20851] (ab218531) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

HEK-293T (human embryonic kidney epithelial cell) transfected with shSEH1L vector, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

false

Exposure time: 3min

Western blot - Anti-SEH1L antibody [EPR20851] (AB218531)
  • WB

Supplier Data

Western blot - Anti-SEH1L antibody [EPR20851] (AB218531)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID : 28199315).

All lanes:

Western blot - Anti-SEH1L antibody [EPR20851] (ab218531) at 1/1000 dilution

All lanes:

Mouse liver lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-SEH1L antibody [EPR20851] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20851

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEH1L also known as SEH1 Nucleoporin is a protein that plays a mechanical role in the nuclear pore complex (NPC) which is involved in the transport of molecules between the nucleus and cytoplasm. It has a molecular mass of approximately 40 kDa. SEH1L is expressed in various tissues with notable expression in the liver kidney and spleen. In the cellular context it associates with other nucleoporins to form the NPC that spans the nuclear envelope facilitating the exchange of macromolecules.
Biological function summary

SEH1L contributes to the structural framework of the nuclear pore complex by being a part of the Nup107-160 subcomplex. This subcomplex is vital for maintaining NPC assembly and function. In addition to its structural role SEH1L influences the timing of mitotic spindle assembly and chromosome segregation. The protein is also implicated in the regulation of the cell cycle showcasing its importance in normal cellular operations.

Pathways

SEH1L has involvement in the mTOR signaling pathway and the cell cycle control pathway. Within the mTOR pathway it works in coordination with proteins such as Raptor and mTORC1 linking nutrient availability to cell growth. In the cell cycle control pathway SEH1L impacts transitions between different phases by interacting with cyclin-dependent kinases and other regulatory proteins. These interactions highlight its role in ensuring proper cell cycle progression and preventing aberrant cell division.

SEH1L is associated with cancer and developmental disorders. In cancer alterations in SEH1L and its related proteins like mTOR can lead to uncontrolled cellular proliferation and tumor growth. Developmental disorders related to faulty NPC components including SEH1L affect nucleocytoplasmic transport which may result in developmental delays and congenital anomalies. Understanding the function and regulation of SEH1L in these contexts offers potential insights for therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC (PubMed : 15146057, PubMed : 17363900). The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore (PubMed : 15146057, PubMed : 17363900).. As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway (PubMed : 23723238, PubMed : 25457612, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex (PubMed : 23723238, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1 (PubMed : 36528027). In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation (PubMed : 36528027). In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex (PubMed : 25457612, PubMed : 26972053, PubMed : 27487210). Within the GATOR2 complex, SEC13 and SEH1L are required to stabilize the complex (PubMed : 35831510).
See full target information SEH1L
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:493-500 PubMed40836086

2025

Structural basis for the dynamic regulation of mTORC1 by amino acids.

Applications

Unspecified application

Species

Unspecified reactive species

Max L Valenstein,Maximilian Wranik,Pranav V Lalgudi,Karen Y Linde-Garelli,Yuri Choi,Raghu R Chivukula,David M Sabatini,Kacper B Rogala

Proceedings of the National Academy of Sciences of the United States of America 121:e2322755121 PubMed39163330

2024

Rag-Ragulator is the central organizer of the physical architecture of the mTORC1 nutrient-sensing pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Max L Valenstein,Pranav V Lalgudi,Xin Gu,Jibril F Kedir,Martin S Taylor,Raghu R Chivukula,David M Sabatini

Nature communications 14:8464 PubMed38123554

2023

VWCE modulates amino acid-dependent mTOR signaling and coordinates with KICSTOR to recruit GATOR1 to the lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Zhao,Yuanyuan Guan,Chenchen Xu,Dong Wang,Jialiang Guan,Ying Liu

International journal of molecular sciences 24: PubMed37686205

2023

SP1-Induced Upregulation of LncRNA AFAP1-AS1 Promotes Tumor Progression in Triple-Negative Breast Cancer by Regulating mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyuan Li,Daheng Xian,Junying Huang,Longzhu Nie,Ting Xie,Qiang Sun,Xiaohui Zhang,Yidong Zhou

Nature 620:200-208 PubMed37407815

2023

SLC38A2 and glutamine signalling in cDC1s dictate anti-tumour immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Chuansheng Guo,Zhiyuan You,Hao Shi,Yu Sun,Xingrong Du,Gustavo Palacios,Cliff Guy,Sujing Yuan,Nicole M Chapman,Seon Ah Lim,Xiang Sun,Jordy Saravia,Sherri Rankin,Yogesh Dhungana,Hongbo Chi

Journal of cell science 136: PubMed37305998

2023

Y-complex nucleoporins independently contribute to nuclear pore assembly and gene regulation in neuronal progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Clarisse Orniacki,Annalisa Verrico,Stéphane Pelletier,Benoit Souquet,Fanny Coulpier,Laurent Jourdren,Serena Benetti,Valérie Doye

Cell reports 42:112316 PubMed36995937

2023

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Yin,Liu Liu,Lauren E Ball,Yalong Wang,Mark T Bedford,Stephen A Duncan,Haizhen Wang,Wenjian Gan

JCI insight 7: PubMed36282590

2022

GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion-positive rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline Morales,David V Allegakoen,José A Garcia,Kristen Kwong,Pushpendra K Sahu,Drew A Fajardo,Yue Pan,Max A Horlbeck,Jonathan S Weissman,W Clay Gustafson,Trever G Bivona,Amit J Sabnis

Nature 607:610-616 PubMed35831510

2022

Structure of the nutrient-sensing hub GATOR2.

Applications

Unspecified application

Species

Unspecified reactive species

Max L Valenstein,Kacper B Rogala,Pranav V Lalgudi,Edward J Brignole,Xin Gu,Robert A Saxton,Lynne Chantranupong,Jonas Kolibius,Jan-Philipp Quast,David M Sabatini

Nature 600:308-313 PubMed34795452

2021

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Long,Jun Wei,Seon Ah Lim,Jana L Raynor,Hao Shi,Jon P Connelly,Hong Wang,Cliff Guy,Boer Xie,Nicole M Chapman,Guotong Fu,Yanyan Wang,Hongling Huang,Wei Su,Jordy Saravia,Isabel Risch,Yong-Dong Wang,Yuxin Li,Mingming Niu,Yogesh Dhungana,Anil Kc,Peipei Zhou,Peter Vogel,Jiyang Yu,Shondra M Pruett-Miller,Junmin Peng,Hongbo Chi
View all publications
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