Anti-SEH1L antibody [EPR20851] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840)

SEH1L was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate with ab218531 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218531 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1 : HEK-293T whole cell lysate 10 μg (Input).
Lane 2 : ab218531 IP in HEK-293T whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab218531 in HEK-293T whole cell lysate (-).
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 10 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID : 28199315).

According to the data of WB1, the band above 250 kDa could be protein aggregates containing SEH1L.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218531).

All lanes:

Immunoprecipitation - Anti-SEH1L antibody [EPR20851] (<a href='/en-us/products/primary-antibodies/seh1l-antibody-epr20851-ab218531'>ab218531</a>)

Predicted band size: 40 kDa

false

• WB

Supplier Data

Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID : 28199315). The band above 250 kDa was also decreased in the SEH1L knockdown lysate. This suggests that the band contains SEH1L, possibly in protein aggregates.

The cell lysates were kindly provided by our collaborator Dr. Liang Zhang, Xiamen University.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218531).

All lanes:

Western blot - Anti-SEH1L antibody [EPR20851] (<a href='/en-us/products/primary-antibodies/seh1l-antibody-epr20851-ab218531'>ab218531</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

HEK-293T (human embryonic kidney epithelial cell) transfected with shSEH1L vector, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

false

Exposure time: 3min