Rabbit Recombinant Monoclonal SEH1L antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.
Images
![Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840), expandable thumbnail](https://content.abcam.com/products/images/seh1l-antibody-epr20851-bsa-and-azide-free-ab235840--immunoprecipitation-img4011.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840)")
![Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840), expandable thumbnail](https://content.abcam.com/products/images/seh1l-antibody-epr20851-bsa-and-azide-free-ab235840--western-blot-img10241.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC (PubMed:15146057, PubMed:17363900). The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore (PubMed:15146057, PubMed:17363900). As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway (PubMed:23723238, PubMed:25457612, PubMed:27487210, PubMed:35831510, PubMed:36528027). The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex (PubMed:23723238, PubMed:27487210, PubMed:35831510, PubMed:36528027). GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1 (PubMed:36528027). In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation (PubMed:36528027). In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex (PubMed:25457612, PubMed:26972053, PubMed:27487210). Within the GATOR2 complex, SEC13 and SEH1L are required to stabilize the complex (PubMed:35831510).
Alternative names
SEC13L, SEH1, SEH1L, Nucleoporin SEH1, GATOR2 complex protein SEH1, Nup107-160 subcomplex subunit SEH1, SEC13-like protein
Recommended products
Rabbit Recombinant Monoclonal SEH1L antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20851
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab235840 is the carrier-free version of Anti-SEH1L antibody [EPR20851] ab218531.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEH1L also known as SEH1 Nucleoporin is a protein that plays a mechanical role in the nuclear pore complex (NPC) which is involved in the transport of molecules between the nucleus and cytoplasm. It has a molecular mass of approximately 40 kDa. SEH1L is expressed in various tissues with notable expression in the liver kidney and spleen. In the cellular context it associates with other nucleoporins to form the NPC that spans the nuclear envelope facilitating the exchange of macromolecules.
- Biological function summary
SEH1L contributes to the structural framework of the nuclear pore complex by being a part of the Nup107-160 subcomplex. This subcomplex is vital for maintaining NPC assembly and function. In addition to its structural role SEH1L influences the timing of mitotic spindle assembly and chromosome segregation. The protein is also implicated in the regulation of the cell cycle showcasing its importance in normal cellular operations.
- Pathways
SEH1L has involvement in the mTOR signaling pathway and the cell cycle control pathway. Within the mTOR pathway it works in coordination with proteins such as Raptor and mTORC1 linking nutrient availability to cell growth. In the cell cycle control pathway SEH1L impacts transitions between different phases by interacting with cyclin-dependent kinases and other regulatory proteins. These interactions highlight its role in ensuring proper cell cycle progression and preventing aberrant cell division.
- Associated diseases and disorders
SEH1L is associated with cancer and developmental disorders. In cancer alterations in SEH1L and its related proteins like mTOR can lead to uncontrolled cellular proliferation and tumor growth. Developmental disorders related to faulty NPC components including SEH1L affect nucleocytoplasmic transport which may result in developmental delays and congenital anomalies. Understanding the function and regulation of SEH1L in these contexts offers potential insights for therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840), expandable thumbnail](https://content.abcam.com/products/images/seh1l-antibody-epr20851-bsa-and-azide-free-ab235840--immunoprecipitation-img4011.jpg "Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)")
Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)
SEH1L was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate with Anti-SEH1L antibody [EPR20851] ab218531 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-SEH1L antibody [EPR20851] ab218531 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate 10 μg (Input).
Lane 2: Anti-SEH1L antibody [EPR20851] ab218531 IP in HEK-293T whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SEH1L antibody [EPR20851] ab218531 in HEK-293T whole cell lysate (-).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.The molecular mass observed is consistent with what has been described in the literature (PMID: 28199315).
According to the data of WB1, the band above 250 kDa could be protein aggregates containing SEH1L.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SEH1L antibody [EPR20851] ab218531).
All lanes: Immunoprecipitation - Anti-SEH1L antibody [EPR20851] (Anti-SEH1L antibody [EPR20851] ab218531)
Predicted band size: 40 kDa
![Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840), expandable thumbnail](https://content.abcam.com/products/images/seh1l-antibody-epr20851-bsa-and-azide-free-ab235840--western-blot-img10241.jpg "Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)")
Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)
Blocking/Dilution buffer: 5% NFDM/TBST.The molecular mass observed is consistent with what has been described in the literature (PMID: 28199315). The band above 250 kDa was also decreased in the SEH1L knockdown lysate. This suggests that the band contains SEH1L, possibly in protein aggregates.
The cell lysates were kindly provided by our collaborator Dr. Liang Zhang, Xiamen University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SEH1L antibody [EPR20851] ab218531).
All lanes: Western blot - Anti-SEH1L antibody [EPR20851] (Anti-SEH1L antibody [EPR20851] ab218531) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg
Lane 2: HEK-293T (human embryonic kidney epithelial cell) transfected with shSEH1L vector, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Exposure time: 3min
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