AB154066

Anti-SEI-1 antibody

Name: Anti-SEI-1 antibody (ab154066) | Abcam
Brand: Abcam Limited
SKU: AB154066
Price: 535 USD
Availability: InStock

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(3 Publications )

Rabbit Polyclonal SEI-1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERTAD1 aa 1-50.

View Alternative Names

SEI1, TRIPBR1, SERTAD1, SERTA domain-containing protein 1, CDK4-binding protein p34SEI1, Transcriptional regulator interacting with the PHD-bromodomain 1, SEI-1, p34(SEI-1), TRIP-Br1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEI-1 antibody (AB154066)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEI-1 antibody (AB154066)

Immunohistochemical analysis of paraffin embedded Human adrenal gland tumor tissue, labeling SEI-1 using ab154066 at 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SERTAD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UHV2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEI-1 also known as p34SEI-1 or CDK4-binding protein p34 is an important protein involved in cell cycle regulation. It has a molecular mass of approximately 31 kDa. Expression of SEI-1 occurs in various tissues with a noticeable presence in proliferative tissues like the thymus spleen and certain cancerous tissues. It exerts its role mechanistically by interacting with cyclin-dependent kinases (CDKs) particularly CDK4 and modulating their activity during cell cycle progression.

Biological function summary

SEI-1 influences cell cycle regulation and proliferation. It acts as a regulatory factor for the CDK4/Cyclin D1 complex facilitating the G1 to S phase transition in the cell cycle. This protein by interacting directly with CDK4 plays a part in the assembly and activation of CDK4 complexes which are pivotal for proper cell division. SEI-1 also has a role outside the cell cycle like apoptosis regulation hinting at diverse functionality within cellular processes.

Pathways

SEI-1 sits prominently in the cell cycle control pathway. It associates closely with the retinoblastoma protein (Rb) pathway where its modulation of CDK4 activity influences Rb phosphorylation. SEI-1’s interaction with CDK4 links it to both the MAP kinase (MAPK) and PI3K/Akt signaling pathways impacting cell growth and survival. Through these associations SEI-1 contributes importantly to the regulation of cellular proliferation and survival mechanisms.

SEI-1 shows connections to cancer particularly in its overexpression found in several cancer types such as breast cancer and gliomas. Its ability to amplify CDK4 activity and deregulate the cell cycle links SEI-1 with oncogenesis. Therapeutic interest targets its interaction with CDK4 to control cancer cell proliferation. SEI-1 also associates with diabetes as its pathway involvement connects to insulin signaling potentially influencing insulin resistance. In both conditions the protein may serve as a potential biomarker or target for new therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts at E2F-responsive promoters as coregulator to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. Stimulates E2F1/TFDP1 transcriptional activity. Renders the activity of cyclin D1/CDK4 resistant to the inhibitory effects of CDKN2A/p16INK4A.

See full target information SERTAD1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 41:e110777 PubMed35993436

2022

Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Volkmar,Christian M Gawden-Bone,James C Williamson,Jonathon Nixon-Abell,James A West,Peter H St George-Hyslop,Arthur Kaser,Paul J Lehner

Molecules and cells 45:216-230 PubMed35014620

2022

Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxiong Li,Bin Li,Yujie Bu,Hailin Zhang,Jia Guo,Jianping Hu,Yanfang Zhang

International journal of cancer 144:558-568 PubMed30230528

2018

Sertad1 promotes prostate cancer progression through binding androgen receptor ligand binding domain.

Applications

Unspecified application

Species

Unspecified reactive species

Bingqing Hu,Haidi Hu,Mingzhu Yin,Zhengrong Sun,Xiaoya Chen,Ying Li,Ziyi Sun,Cong Liu,Ling Li,Yang Qiu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEI-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEI-1 antibody (AB154066)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise