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AB137845

Anti-Seladin 1 antibody

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(3 Publications)

Rabbit Polyclonal Seladin 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHCR24 aa 50-400.

View Alternative Names

KIAA0018, DHCR24, Delta(24)-sterol reductase, 24-dehydrocholesterol reductase, 3-beta-hydroxysterol Delta-24-reductase, Diminuto/dwarf1 homolog, Seladin-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Seladin 1 antibody (AB137845)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Seladin 1 antibody (AB137845)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cell labelling Seladin 1 with ab137845 at a 1/200 dilution. Lower panel merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody (AB137845)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody (AB137845)

Immunohistochemical analysis of paraffin embedded SW480 xenograft labelling Seladin 1 with ab137845 at a 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DHCR24 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

Q15392

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Seladin 1 also known as DHCR24 (3β-Hydroxysteroid-Δ24 reductase) is an enzyme with a molecular mass of approximately 54 kDa. It plays a role in cholesterol biosynthesis by catalyzing the reduction of a double bond at the C-24 position in sterol precursors. Seladin 1 is expressed in various tissues with high expression in the brain liver and adrenal gland. The enzyme contributes to the maintenance of cellular cholesterol homeostasis and provides neuroprotective functions.
Biological function summary

Seladin 1 plays an important role in cellular processes particularly in response to oxidative stress. The protein is not part of a larger complex but functions independently to reduce highly reactive oxygen species levels in cells. It helps in maintaining cellular integrity and contributes to the survival of neuronal cells under stress conditions. Additionally it provides resistance to oxidative stress-induced apoptosis by modulating intracellular antioxidative defense mechanisms.

Pathways

Seladin 1 plays an integral role in the cholesterol biosynthesis pathway and is closely associated with the mevalonate pathway. Its activity directly influences the availability of cholesterol and other sterols impacting membrane fluidity and cellular functions dependent on sterol concentrations. The enzyme interacts with related proteins such as HMG-CoA reductase an important regulatory enzyme within the cholesterol synthesis pathway. These interactions ensure that cellular cholesterol levels are well-regulated to meet physiological demands.

Seladin 1 has been implicated in Alzheimer's disease and certain cancers. In Alzheimer's disease a reduction in Seladin 1 expression correlates with increased susceptibility to amyloid-beta toxicity while its overexpression shows a protective effect. This relationship ties Seladin 1 to amyloid precursor protein processing. In some cancers altered Seladin 1 expression affects tumor growth and progression where it may interact with proteins involved in cell survival pathways. This dual role in both neurodegenerative and cancerous conditions highlights the importance of Seladin 1 in human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis (PubMed : 11519011, PubMed : 21671375, PubMed : 22178193, PubMed : 25637936). In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress (PubMed : 11007892, PubMed : 22010141). Also protects against amyloid-beta peptide-induced apoptosis (PubMed : 11007892).
See full target information DHCR24
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 11:102 PubMed37344916

2023

DHCR24 reverses Alzheimer's disease-related pathology and cognitive impairment via increasing hippocampal cholesterol levels in 5xFAD mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Bin Zhang,Yue Huang,Xiao-Rou Guo,Meng-Qi Zhang,Xiang-Shan Yuan,Heng-Bing Zu

Nature medicine 23:1055-1062 PubMed28805822

2017

Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Pingzhao Zhang,Dejie Wang,Yu Zhao,Shancheng Ren,Kun Gao,Zhenqing Ye,Shangqian Wang,Chun-Wu Pan,Yasheng Zhu,Yuqian Yan,Yinhui Yang,Di Wu,Yundong He,Jun Zhang,Daru Lu,Xiuping Liu,Long Yu,Shimin Zhao,Yao Li,Dong Lin,Yuzhuo Wang,Liguo Wang,Yu Chen,Yinghao Sun,Chenji Wang,Haojie Huang

Neuropathology and applied neurobiology 42:535-46 PubMed26373857

2015

Evidence for altered cholesterol metabolism in Huntington's disease post mortem brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Kreilaus,Adena S Spiro,Catriona A McLean,Brett Garner,Andrew M Jenner
View all publications
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