Anti-Seladin 1 antibody [EPR10743]

• WB

Supplier Data

Western blot - Anti-Seladin 1 antibody [EPR10743] (AB181062)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Seladin 1 antibody [EPR10743] (ab181062) at 1/1000 dilution

Lane 1:

A375 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa

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• WB

Lab

Western blot - Anti-Seladin 1 antibody [EPR10743] (AB181062)

Lanes 1 - 3 : Merged signal (red and green). Green - ab181062 observed at 51 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab181062 was shown to react with DHCR24 in A431 wild-type cells in Western blot. Loss of signal was observed when DHCR24 knockout sample was used. A431 wild-type and DHCR24 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab181062 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Seladin 1 antibody [EPR10743] (ab181062) at 1/2000 dilution

Lane 1:

Wild-type A431 whole cell lysate at 20 µg

Lane 2:

DHCR24 knockout A431 whole cell lysate at 20 µg

Lane 2:

Western blot - Human DHCR24 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-dhcr24-knockout-a-431-cell-line-ab262480'>ab262480</a>)

Lane 3:

HepG2 whole cell lysate at 20 µg

Predicted band size: 60 kDa

Observed band size: 51 kDa

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